Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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DA-9601, Artemisia Asiatica Herbal Extract, Ameliorates Airway Inflammation of Allergic Asthma in Mice

  • Kim, Ji Young (Department of Pharmacology, Sungkyunkwan University School of Medicine) ;
  • Kim, Dae Yong (Department of Pharmacology, Sungkyunkwan University School of Medicine) ;
  • Lee, Yun Song (Department of Pharmacology, Sungkyunkwan University School of Medicine) ;
  • Lee, Bong Ki (Department of Microbiology, Yonsei University College of Medicine) ;
  • Lee, Kyung-Hoon (Department of Pharmacology, Sungkyunkwan University School of Medicine) ;
  • Ro, Jai Youl (Department of Pharmacology, Sungkyunkwan University School of Medicine)
  • Received : 2006.07.10
  • Accepted : 2006.07.14
  • Published : 2006.08.31
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Abstract

We previously reported that DA-9601, ethanol herbal extract of Artemisia asiatica, inhibited histamine and leukotriene releases in guinea pig lung mast cells activated with specific antigen/antibody reaction. This study aimed to evaluate the inhibitory effect of DA-9601 on the OVA-induced airway inflammation in allergic asthma mouse model. BALB/c mice were sensitized and challenged with OVA. DA-9601 was administered orally 1 h before every local OVA-challenge. OVA-specific serum IgE was measured by ELISA, recruitment of inflammatory cells in BAL fluids and lung tissues by Diff-Quik and H&E staining, respectively, the expressions of CD40, CD40L and VCAM-1 by immunohistochemistry, goblet cell hyperplasia by PAS staining, activities of MMPs by gelatin zymography, expressions of mRNA and proteins of cytokines by RT-PCR and ELISA, activities of MAP kinases by western blot, and activity of NF-${\kappa}B$ by EMSA. DA-9601 reduced IgE level, recruitment of inflammatory cells into the BAL fluid and lung tissues, expressions of CD40, CD40L and VCAM-1 molecules, goblet cell hyperplasia, MMPs activity, expressions of mRNA and productions of various cytokines, activities of MAP kinases and NK-${\kappa}B$ increased from OVA-challenged mice. These data suggest that DA-9601 may be developed as a clinical therapeutic agent in allergic diseases due to suppressing the airway allergic inflammation via regulation of various cellular molecules expressed by MAP kinases/NF-${\kappa}B$ pathway.

Keywords

Acknowledgement

Supported by : Sungkyunkwan University

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