Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Effect of 1-Methylcyclopropene Treatment on Quality and Ethylene Production of Muskmelon (Cucumis melo L. cv. Reticulatus) Fruit

1-Methylcyclopropene 처리가 머스크멜론 과실의 에틸렌 발생과 품질에 미치는 영향

Cha, Ju-Hyeong;Lee, Geung-Pyo;Hwang, Byeong-Ho;Kim, Jong-Ki;Lee, Eun-Ji
차주형;이긍표;황병호;김종기;이은지

  • Published : 20060000
⟨ Previous Next ⟩

Abstract

Before the onset of climacteric, mature muskmelon fruit (var. ‘Pearl’) grown and harvested from a plastic house was applied with 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene. And its subsequent molecular metabolic changes including expression of genes involved in biosynthesis and perception of ethylene were monitored during storage at 25℃. Fruit treated with 1.0 μLL-1 of 1-MCP for 12 hours displayed a very slow process in ripening-associated physiological changes, compared to fruit provided with air, served as a control. Only a slight increment in ethylene production, accompanied by a significant delay of tissue softening and of yellow color development in fruit skin was manifested by 1-MCP treatment. However, change of total soluble solids content from the fruit either treated with the inhibitor or air remained fairly constant 4 days after the inhibitor treatment. The application of 1-MCP to the muskmelon fruit reduced the expression of CM-ACS1 and delayed the normal expression of CM-ACO1 when compared to the control. However, expression of genes involved in ethylene reception, CM-ETR1 and CM-ERS1, remained fairly unaffected for 15 days of storage after 1-MCP application. These data obtained in the present study support that 1-MCP blocks ethylene binding to its receptor and thus, inhibiting the action of ethylene, consequently suppressing the autocatalytic expression of CM-ACS1. A relatively small peak in ethylene production displayed by 1-MCP treatment can be ascribed to reduced amounts of expression of CM-ACS1 rather than CM-ACO1, lending to inhibition of an autocatalytic production of ethylene, which is a prerequisite for climacteric fruit to undergo ripening.

본 연구는 네트멜론 ‘Pearl’ 과실을 공시하여 1μLL-1 농도로 12시간 1-MCP를 처리한 후 25℃에서 저장하며 에틸렌 생합성과 관련된 유전자들의 발현양상과 과실의 품질변화를 조사하였다. 과실의 경도는 무처리구에서는 처리 96시간 경과 후에 8.4N에서 2.6N으로 급격하게 낮아졌으나, 1-MCP처리구에서는 360시간 경과 후에도 3.9N를 유지하였다. 과실의 색도(Hunter ‘a’, ‘b’ 값)는 무처리구에서는 처리 후 72시간이 경과하면서 과피가 급속히 노랗게 착색되었으나, 1-MCP 처리구에서는 동기간 동안 완만하게 노란색이 증가하였다. 과실의 당도는 무처리구에서 10.1oBrix로 급격히 증가한 다음 그 이후에는 약간 감소한 반면에, 처리구에서는 완만하게 증가한 뒤 9.0oBrix 정도로 유지되다가 이후 7.0oBrix로 감소하였다. 한편, 과실의 에틸렌 발생은 무처리구에서는 96시간 경과 후 몇시간에 걸쳐 에틸렌이 급격히 증가하는 전형적인 climacteric 현상을 보였으나, 처리구에서는 360시간이 지나도록 2μLkg-1h-1 이하로 유지되었으며, 일시적인 증가는 나타나지 않았다. 1-MCP처리구에서 ripening 중 에틸렌 생합성에 관련된 유전자인 CM-ACS1과 CM-ACO1의 발현양상은 무처리구에 비해 전체적으로 정량적으로 감소하는 경향이었으며, 동시에 발현이 증가되는 시기도 지연되었다. 그러나 에틸렌 수용단백질인 CM-ETR1과 CM-ERS1은 처리구에서 발현이 다소 지연되는 것을 관찰할 수 있었으나 발현양에서는 큰 차이를 보이지 않았다. 본 연구로부터 1-MCP는 에틸렌 수용체와 결합하여 에틸렌의 자가촉매현상(system 2)를 억제함으로써 멜론과실의 에틸렌 발생과 조직의 연화를 지연시켰다. 한편 과실의 당도도 별로 영향을 받지 않았다.

Keywords

References

  1. Abeles, F.B., P.W. Morgan, and M.E. Saltveit Jr. 1992. Ethylene in plant biology. 2nd ed. p. 414. San Diego, CA: Academic Press
  2. Akira. N., S. Murachi, H. Okunishi, S. Shiomi, R. Nakano, Y. Kubo, and A. Inaba. 1998. Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase and ethylene receptor genes in tomato fruit during development and ripening. Plant Physiol. 118:1295-1305 https://doi.org/10.1104/pp.118.4.1295
  3. Barry, C.S., M.I. Llop-Tous, and D. Grierson. 2000. The regulation of 1-aminocyclopropane-1-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiol. 123:979-986 https://doi.org/10.1104/pp.123.3.979
  4. Blankenship, S.M. and J.M. Dole. 2003. 1-Methylcyclopropene: A review. Postharvest Biol. Technol. 28:1-25 https://doi.org/10.1016/S0925-5214(02)00246-6
  5. Bleecker, A.B. and H. Kende. 2000. Ethylene: A gaseous signal molecule in plants. Annu. Rev. Cell Dev. Biol. 16:1-18 https://doi.org/10.1146/annurev.cellbio.16.1.1
  6. Bregoli. A.M., V. Ziosi, S. Biondi, A. Rasori, M. Ciccioni, G. Costa, and P. Torrigiani. 2005. Postharvest 1-methylcyclopropene application in ripening control of 'Stark Red Gold' nectarines: Temperature-dependent effects on ethylene production and biosynthetic gene expression, fruit quality, and polyamine levels. Postharvest Biol. Technol. 37:111-121 https://doi.org/10.1016/j.postharvbio.2005.04.006
  7. Dong, L., S. Lurie, and H.W. Zhou. 2002. Effect of 1-methylcyclopropene on ripening of 'Canino' apricots and 'Royal Zee' plums. Postharvest Biol. Technol. 24:135-145 https://doi.org/10.1016/S0925-5214(01)00130-2
  8. Fan, X., L. Argenta, and J.P. Mattheis. 2002. Interactive effects of 1-MCP and temperature on 'Elberta' peach quality. HortScience 37:134-138
  9. Giovannoni, J.J. 2004. Genetic regulation of fruit development and ripenig. Plant Cell. 16:170-180 https://doi.org/10.1105/tpc.019158
  10. Kader, A.A. 2002. Postharvest biology and technology: An overview. In: Postharvest Technology of Horticultural Crops. 3rd ed. p. 15-20. Agriculture & Natural Resources
  11. Lasserre, E., T. Bouquin, J.A. Hernandez, J. Bull, J.C. Pech, and C. Balagué. 1996. Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.). Mol. Gen. Genet. 251:81-90
  12. Liguori, G., A. Weksler, Y. Zutahi, S. Lurie, and Kosto. 2004. Effect of 1-methylcyclopropene on ripening of melting flesh peaches and nectarines. Postharvest Biol. Technol. 31:263-268 https://doi.org/10.1016/j.postharvbio.2003.09.007
  13. Nakatsuka A., S. Murachi, H. Okunishi, S. Shiomi, R. Nakano, Y. Kubo, and A. Inaba. 1998. Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening. Plant Physiol. 118:1295-1305 https://doi.org/10.1104/pp.118.4.1295
  14. Phillips, L.A., C. Barry, and J. Giovannoni. 2004. Signal transduction systems regulating fruit ripening. Trends Plant Sci. 9:331-338 https://doi.org/10.1016/j.tplants.2004.05.004
  15. Porat, R., E. Shlomo, M. Serek, E.C. Sisler, and A. Borochov. 1995. 1-Methylcyclopropene inhibits ethylene action in cut phlox flowers. Postharvest Biol. Technol. 6:313-319 https://doi.org/10.1016/0925-5214(95)00014-W
  16. Roh, K.A., K.C. Son, Y.H. Lim, S.E. Oh, B.C. In, and E.C. Sisler. 2001. Effect of 1-MCP and its derivatives on ethylene binding in banana ripening. J. Kor. Soc. Hort. Sci. 42:458-461
  17. Sato-Nara, K., K.I. Yuhashi, K. Higashi, K. Hosoya, M. Kubota, and H. Ezura. 1999. Stage- and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon (Cucumis melo L.) isozymes. J. Amer. Soc. Hort. Sci. 123:264-272
  18. Serek, M., E.C. Sisler, and M.S. Reid. 1994. Novel gaseous ethylene binding inhibitor prevents ethylene effect in potted flowering plants. J. Amer. Soc. Hort. Sci. 119:1230-1233
  19. Sisler, E.C. and M. Serek. 1997. Inhibitors of ethylene responses in plants at the receptor level: Recent development. Physiol. Plant. 100:577-582 https://doi.org/10.1111/j.1399-3054.1997.tb03063.x
  20. Wills, R.B.H. and V.V.V. Ku, 2002. Use of 1-MCP to extend the time to ripen of green tomatoes and postharvest life of ripe tomatoes. Postharvest Biol. Technol. 26:85-90 https://doi.org/10.1016/S0925-5214(01)00201-0
  21. Yamamoto, M., T. Miki, Y. Ishiki, K. Fujinami, Y. Yanagisawa, H. Nakagawa, N. Ogura, T. Hirabayashi, and T. Sato. 1995. The synthesis of ethylene in melon fruit during the early stage of ripening. Plant Cell Physiol. 36:591-596
  22. Zhong, G.Y., M. Huberman, X.Q. Feng, E.C. Sisler, D. Holland, and R. Goren. 2001. Effect of 1-methylcyclopropene on ethylene-induced abscission in citrus. Physiol. Plant. 113:134-141 https://doi.org/10.1034/j.1399-3054.2001.1130118.x