Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
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Mycoplasma pneumoniae-induced production of proasthmatic mediators in airway epithelium

인체 기관지 상피세포에서 Mycoplasma pneumoniae 감염에 의한 천식 매개물질의 발현

  • Kim, Kyung Won (Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine and Rickettsial and Zoonotic Diseases Division) ;
  • Lee, Byung Chul (Department of Microbiology, National Institute of Health) ;
  • Lee, Kyung Eun (Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine and Rickettsial and Zoonotic Diseases Division) ;
  • Kim, Eun Soo (Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine and Rickettsial and Zoonotic Diseases Division) ;
  • Song, Tae Won (Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine and Rickettsial and Zoonotic Diseases Division) ;
  • Park, Mi Yeoun (Department of Microbiology, National Institute of Health) ;
  • Sohn, Myung Hyun (Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine and Rickettsial and Zoonotic Diseases Division) ;
  • Kim, Kyu-Earn (Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine and Rickettsial and Zoonotic Diseases Division)
  • 김경원 (연세대학교 의과대학 소아과학교실 및 알레르기 연구소) ;
  • 이병철 (국립보건원 세균부 리케치아과) ;
  • 이경은 (연세대학교 의과대학 소아과학교실 및 알레르기 연구소) ;
  • 김은수 (연세대학교 의과대학 소아과학교실 및 알레르기 연구소) ;
  • 송태원 (연세대학교 의과대학 소아과학교실 및 알레르기 연구소) ;
  • 박미연 (국립보건원 세균부 리케치아과) ;
  • 손명현 (연세대학교 의과대학 소아과학교실 및 알레르기 연구소) ;
  • 김규언 (연세대학교 의과대학 소아과학교실 및 알레르기 연구소)
  • Received : 2006.06.02
  • Accepted : 2006.07.10
  • Published : 2006.09.15
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Abstract

Purpose : There has been an increasing amount of literature concerning the association between Mycoplasma pneumoniae and asthma pathogenesis. Interleukin(IL)-6 stimulates the differentiation of monocytes, and can promote Th2 differentiation and simultaneously inhibit Th1 polarization. IL-8 is a potent chemoattractant and, it has been suggested, has a role in asthma pathogenesis. Nitric oxide (NO) synthesized by airway epithelium may be important in the regulation of airway inflammation and reactivity. Vascular endothelial growth factor(VEGF) has been reported to be a mediator of airway remodeling in asthma. We investigated the effects of M. pneumoniae on IL-6, IL-8, NO and VEGF production in human respiratory epithelial cells. Methods : A549 cells were cultured and inoculated with M. pneumoniae at a dose of 20 cfu/cell. After infection, the presence of M. pneumoniae in epithelial cell cultures was monitored by immunofluorescence and confirmed by polymerase chain reaction(PCR) detection. IL-6, IL-8 and VEGF were determined by an enzyme-linked immunosorbent assay and reverse transcriptase-polymerase chain reaction. NO was measured using the standard Griess reaction. Results : In A549 cells, M. pneumoniaeinduced IL-6, IL-8, NO and VEGF release in time-dependent manners. It also induced mRNA expression of IL-6, IL-8 and VEGF in similar manners. Conclusion : These observations suggest that M. pneumoniae might have a role in the pathogenesis of the allergic inflammation of bronchial asthma.

목 적 : M. pneumoniae는 기도 점막의 상피세포에서 증식하면서 호흡기 질환을 일으키며 기관지 천식의 발생이나 악화와 관계된다고 알려져 있다. IL-6은 급성 염증을 유도하는 사이토카인으로 B 세포의 분화에 관여하며 Th2 염증반응을 촉진시키며, IL-8은 기도에서 염증부위로의 세포이동을 매개하는 케모카인으로 알레르기 염증 반응에 밀접한 관련을 가진다. 기도 상피세포에서 생성되는 NO는 기도 염증과 기도과민성의 조절에 중요하다. VEGF는 천식에서 기도개형에 주된 역할을 담당한다. 본 연구에서는 기관지 상피세포에 M. pneumoniae를 감염시켰을 때 IL-6, IL-8, NO 및 VEGF의 발현을 살펴보았다. 방 법 : M. pneumoniae를 기관지 상피 세포주인 A549 세포에 감염시킨 후 여러 시간 간격으로 배양하여 세포와 배양 상층액을 수거하였다. 면역 형광 염색과 M. pneumoniae 16S ribosomal RNA gene의 oligonucleotide primers를 이용한 중합효소 연쇄반응을 시행하여 감염을 확인하였다. IL-6, IL-8, VEGF 단백질 생성은 ELISA kit를 이용하여 정량하였고, NO는 Griess 반응을 이용하여 측정하였다. 역전사 중합효소 연쇄반응을 이용하여 IL-6, IL-8, VEGF의 mRNA 발현을 관찰하였다. 결 과 : 기관지 상피세포에 M. pneumoniae를 감염시킨 후 시간이 지남에 따라 IL-6, IL-8, NO, VEGF의 생성이 증가하였고, IL-6, IL-8, VEGF mRNA 발현이 증가됨을 관찰하였다. 결 론 : M. pneumoniae 감염은 IL-6, IL-8, NO, VEGF 등의 매개물질의 발현을 증가시켜 기관지 천식의 알레르기 염증반응에 관여할 것으로 사료된다.

Keywords

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