Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Sinigrin Contents in Different Tissues of Wasabi and Antimicrobial Activity of Their Water Extracts

고추냉이 부위별 Sinigrin 함량과 추출액의 항균 활성

Park, Yun-Yeong;Jo, Mun-Su;Park, Sin;Lee, Yeong-Deuk;Jeong, Byoung-Ryong;Jeong, Jong-Bae
박윤영;조문수;박신;이영득;정병룡;정종배

  • Published : 20060000
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Abstract

Total sinigrin contents in rhizome, leaf petiole and leaf blade of wasabi (Wasabia japonica) were determined by simultaneous measurement of sinigrin and allyl isothiocyanate using HPLC. The antimicrobial activity of water extracts of the different tissues were also examined against Staphylococcus aureus, Streptococcus agalatiae, Listeria monocytogenes, Escherichia coli and Streptococcus mutans. The contents of total sinigrin including allyl isothiocyanate were 15.22, 1.85, 1.95 mgg-1 in rhizome, leaf petiole and leaf blade of wasabi, respectively. The contents were much higher than those found by other researchers, who determined sinigrin and isothiocyanates by measuring content of allyl isothiocyanate after the conversion of sinigrin to allyl isothiocyanate. During the conversion, the loss of the volatile compound iosthiocyanates and the incomplete conversion of sinigrin to allyl isothiocynate might be possible, and these factors may be related to the differences in the total sinigrin measurements. Water extracts of wasabi rhizome, leaf petiole and leaf blade showed anti-microbial activity against Staphylococcus aureus, Streptococcus agalatiae, Listeria monocytogenes and Escherichia coli, and the highest activity was found in the rhizome extract, where the total sinigrin content was much higher than those found in leaf petiole and leaf blade. Although the contents of sinigrin and isothiocyanates in leaf petiole and leaf blade of wasabi are relatively lower than that in rhizome, the biomass of leaf petiole and leaf blade are greater than that of rhizome and also can be continuously harvested from the same root and rhizome system. Therefore, the aboveground parts of wasabi can be used in the extraction of sinigrin and isothiocyanates as commercial antimicrobial compounds.

고추냉이의 근경, 엽병 그리고 잎 중의 sinigrin 및 allyl isothiocyanate 함량을 HPLC를 이용한 동시분석방법으로 측정하였다. 본 연구에서 측정된 총 sinigrin 함량은 sinigrin을 allyl isothiocyanate로 전환시킨 후 allyl isothiocyanate만을 측정한 기존의 연구 결과에 비하여 근경, 엽병, 잎에서 최소 5.9, 3.9, 1.8배 이상 높았다. 분석에 사용된 고추냉이의 품종이나 재배환경의 차이도 있을 수 있지만 기존의 분석방법에서는 불완전한 sinigrin의 가수분해나 휘발성인 allyl isothiocyanate의 손실로 인해 총 sinigrin 함량을 정확히 측정하지 못한 것으로 판단된다. Staphylococcus aureus, Streptococcus agalatiae, Listeria monocytogenes, Escherichia coli 그리고 Streptococcus mutans에 대한 고추냉이 부위별 추출물의 항균 활성을 조사한 결과를 보면 일부 차이는 있었으나 Streptococcus mutans를 제외한 모든 균에 대하여 생육저해 효과를 나타내었다. 특히 근경 추출물은 24시간까지 지속적으로 이들 미생물의 생육을 저해하였다. 이러한 미생물 생육저해효과는 allyl isothiocyanate를 포함한 각종 isothiocyanate의 함량에 따라서 결정되며, 근경 추출물의 항균효과가 큰 이유는 엽병이나 잎에 비하여 isothiocyanate 화합물의 함량이 높기 때문이다. 고추냉이 추출물의 처리가 Streptococcus mutans의 생육을 억제하지 못한 것은 다른 균에 비하여 isothiocyanate에 대한 Streptococcus mutans의 저항력이 크기 때문이다. 고추냉이의 엽병과 잎 중의 sinigrin 또는 isothiocyanate 화합물의 함량은 근경에 비하여 낮았다. 그러나 sinigrin 또는 isothiocyanate 화합물을 추출하여 상업적으로 활용하는데 있어서는 근경보다 생산량이 많고 지속적으로 채취가 가능한 엽병과 잎이 훨씬 유리할 것으로 판단된다.

Keywords

References

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