Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

In Vitro Propagation of Korean Native Winter Hazel (Corylopsis coreana)

한국 특산 히어리의 기내 증식

Ko, Gap-Cheon;Im, Dong-Ok
고갑천;임동옥

  • Published : 20060000
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Abstract

This study was carried out to propagate an endangered Korean winter hazel (Corylopsis coreana) in vitro. It was effective to do suspension-culture 2 mm shoot tips in size on the MS liquid media containing 2 mgL-1 6-benzylaminopurine (BA) for establishing shoots in vitro. Shoots were well multiplicated and grown when subcultured on the MS media containing 1~2 mgL-1 BA + 0.1 mgL-1 α-naphthaleneacetic acid (NAA), while shoots were over-multiplicated when on the MS media containing more than 3 mgL-1 BA. A two-step culture method was used for the rooting of shoots in vitro. In the first rooting step, 1 cm shoot tips or nodes with one leaf were cultured on the 1/4 MS media containing NAA or indole-3-butyric acid (IBA) for 6 days in the dark. Then, they were cultured on 1/4 MS media without growth regulators. The best rooting, 80~100%, was obtained from the shoot tips cultured on the 1/4 MS media containing 1~3 mgL-1 IBA in the first rooting step. The rooted plantlets were acclimatized in the closed box, and then grown in the plastic house.

한국의 특산 수종인 히어리(Corylopsis coreana)의 특성유지를 위한 기내 번식 방법을 확립하고자, 액아의 정단배양, 신초 계대배양과 발근에 미치는 배지 및 생장조절제의 영향을 조사하였다. 2mgL-1 BA를 첨가한 MS 액체배지에 2mm 정단을 치상하여 현탁배양하였을 때 신초발생률이 35%로 가장 높았다. 신초의 증식과 생장은 1~2mgL-1 BA와 0.1mgL-1 NAA를 혼용첨가한 배지가 계대배양 과정에서 신초 증식과 생장에 적합하였으며 BA가 3mgL-1 이상 첨가되면 과도하게 다경이 형성되어 부적합하였다. 발근은 분리한 신초나 외마디를 IBA가 1~3mgL-1 첨가된 1/4MS배지에 치상하고 6일 동안 암처리한 다음 이들을 생장조절제가 없는 1/4MS 배지에 치상하면 80~100%가 발근되었다. 발근된 기내 신초의 순화율은 77.2~73.3%였으며 대부분이 사립형태로 생장하였다.

Keywords

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