Genes and Genomics

The Genetics Society of Korea (한국유전학회)
권호 표지

Characterization of Eight Rumex Species by FISH (Fluorescence in situ Hybridization) and 5S rDNA Spacer Sequences

Kim, Su-Yeong;Choe, Hae-Won;Gu, Dal-Hoe;Lee, U-Gyu;Lee, Jun-Gyu;Bang, Jae-Uk
김수영;최해원;구달회;이우규;이준규;방재욱

  • Published : 20060000
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Abstract

Using both molecular and cytogenetic methods, we have examined and characterized eight Rumex species distributed throughout the Korean Peninsula. The somatic metaphase chromosomes in the Lapathum group were 2n=6x=60 in three of the species (R. crispus, R. japonicus and R. longifolius), 2n=5x=50 in one species (R. nipponicus), and 2n=4x=40 in two of the species (R. obtusifolius and R. maritinus). In the two dioecious groups, the chromosome numbers were 2n=6x=42 (female) and 43 (male) in R. acetocella, and 2n=2x=14 (female) and 15 (male) in R. acetosa. Using the FISH method, we physically mapped the 5S and 45S rDNA genes on the chromosomes of all the studied species. All of the species, except R. japonicus, possessed four 45S rDNA sites, whereas the 5S rDNA gene sites were variable, ranging between 2 and 8. Polymorphic 5S rDNA sites at the same ploidy level were found among species in the subgenus Lapathum group. Nucleotide sequence analysis revealed that the 5S rDNA spacer also varied between the species, ranging between 308 and 315 bp, except for each 120 bp coding region. FISH and 5S rDNA spacer sequencing were successfully applied to the identification and characterization of the eight Rumex species examined in this study.

Keywords

References

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