Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Alteration of Antioxidative System to Chilling Stress in Two Weedy Rice (Oryza sativa L.) Germplasms Contrasting in Sensitivity

감수성이 서로 다른 두 잡초벼 계통에서 저온 스트레스에 대한 항산화계의 변화

Son, Yeong-Geol;Lee, Jeung-Ju;Lee, Yeong-Han;Ha, Jeong-Gi;Nam, Jin-Seon
손영걸;이증주;이영한;하정기;남진선

  • Published : 20060000
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Abstract

We conducted the physiological comparisons to improve understanding of chilling- tolerant mechanisms in the two weedy rice (Oryza sativa L.) germplasms differing in sensitivity, the one (Dalseong-aengmi 44) is tolerant but the other one (Hwaseong-aengmi 4) is sensitivity to chilling. The leaves of Dalseong-aengmi 44 was curled and dehydrated partly by chilling stress, but the damage of Hwaseong-aengmi 4 was little. Under chilling stress, reduction of water content and increase of lipid peroxidation in Dalseong-aengmi 44 were notable more than in Hwaseong- aengmi 4. The negative correlation between shoot proline levels and chilling-tolerance was more evident in Dalseong-aengmi 44. This expreimental evidence indicated that their capacity to accumulate proline under chilling stress was not an indicator of chilling-tolerance, but merely a consequence or a symptom of the stress. The constitutive and chilling-induced activities of antioxidant enzymes, such as superoxide dismutase, ascorbate peroxidase, catalase and glutathione reductase were higher in Hwaseong-aengmi 4 than in Dalseong-aengmi 44. These results suggested that the tolerance to chilling stress in weedy rice is well associated with the constitutive and enhanced capacity of antioxidative enzyme system.

본 연구는 저온 스트레스에 대한 반응성이 서로 다른 잡초성벼의 내한성과 관련된 생리적 요인을 구명하기 위한 연구의 일환으로 수행되었다. 저온 감수성인 달성앵미 44는 내성인 화성앵미 4에 비해 가시적인 피해 현상이 뚜렷하였으며, 지상부 수분함량의 감소도 보다 현저하게 나타났다. 저온 스트레스에 의한 과산화지질의 생성량도 화성앵미 4보다 달성앵미 44에서 높게 나타났는데, 저온 스트레스의 처리기간이 길어질수록 그 차이는 더 커졌다. 삼투 유기용질인 proline함량은 저온 스트레스 하의 두 잡초성벼에서 현저하게 증가하는 경향이었는데, 화성앵미 4보다 달성앵미 44에서의 증가율이 더 높은 것으로 나타났다. 화성앵미 4는 달성앵미 44보다 30 에서 생육된 경우에 SOD, AP CAT 및 GR의 활성이 더 높은 경향이었으며, 2 의 저온 스트레스 하에서도 각 효소의 활성이 더 높은 수준으로 증가하였다. 특히, 화성앵미 4는 달성앵미 44에 비해 저온처리 72시간과 24시간 후에 SOD와 CAT의 활성이 현저하게 증가하였다.

Keywords

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