Journal of Trauma and Injury

The Korean Society of Traumatology (대한외상학회)
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The Effect of Hypothermia on Lung Inducible Nitric Oxide Synthase Gene Expression in Intestinal Ischemia-Reperfusion Injury

장 허혈-재관류에서 폐조직의 Inducible Nitric Oxide Synthase유전자 발현에 대한 저체온증의 효과

  • Kim, Kyuseok (Department of Emergency Medicine, Seoul National University College of Medicine) ;
  • Lee, Jeong Hun (Department of Emergency Medicine, Seoul National University College of Medicine) ;
  • Suh, Gil Joon (Department of Emergency Medicine, Seoul National University College of Medicine) ;
  • Youn, Yeo Kyu (Department of Surgery, Seoul National University College of Medicine) ;
  • Kang, Young Joon (Department of Emergency Medicine, Cheju National University College of Medicine) ;
  • Kim, Min A (Department of Pathology, Seoul National University College of Medicine) ;
  • Cho, Sang-Gi (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonbuk National University) ;
  • Shin, Hyo-Keun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonbuk National University)
  • 김규석 (서울대학교 의과대학 응급의학과) ;
  • 이정훈 (서울대학교 의과대학 응급의학과) ;
  • 서길준 (서울대학교 의과대학 응급의학과) ;
  • 윤여규 (서울대학교 의과대학 외과) ;
  • 강영준 (제주대학교 의과대학 응급의학과) ;
  • 김민아 (서울대학교 의과대학 병리학교실) ;
  • 조상기 (전북대학교 치과대학 구강악안면외과학교실) ;
  • 신효범 (전북대학교 치과대학 구강악안면외과학교실)
  • Received : 2006.05.12
  • Accepted : 2006.06.14
  • Published : 2006.06.30
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Abstract

Purpose: Although hypothermia has been used in many clinical situations, such as post cardiopulmonary resuscitation, stroke, traumatic brain injury, septic shock, and hemorrhagic shock, the mechanism by which it works has not been clearly elucidated. We aimed to evaluate the effect of hypothermia on the plasma nitric oxide (NO) concentration, lung iNOS expression, and histologic changes in intestinal ischemia-reperfusion (IR). Method: Male Sprague-Dawley rats were randomly divided into the hypothermia group (HT, n=8, $27{\sim}30^{\circ}C$) and the normothermia group (NT, n=8, $36{\sim}37^{\circ}C$). They underwent 30 min of intestinal ischemia by clamping the superior mesenteric artery, which was followed by 1.5 h of reperfusion. They were then sacrificed. The acute lung injury (ALI) score, the plasma NO concentration, and lung iNOS gene expression were measured. Results: Compared with the HT group, the NT group showed severe infiltrations of inflammatrory cells, alveolar hemorrhages, and interstitial hypertrophies in lung tissues. There were significant differences in the ALI scores between the NT and the HT groups ($8.7{\pm}1.5/HPF$ in NT vs $5.8{\pm}1.2/HPF$ in HT, p=0.008). Although the plasma NO concentration was slightly lower in the HT group, there was no significant difference between the two groups ($0.80{\pm}0.24{\mu}mol/L$ in NT vs $0.75{\pm}0.30{\mu}mol/L$ in HT, p=0.917). Lung iNOS gene expression was stronger in the NT group than in the HT group. The band density of the expression of iNOS in lung tissues was significantly increased in the NT group compared to the HT group ($5.54{\pm}2.75$ in NT vs$0.08{\pm}0.52$ in HT, p=0.002). Conclusions: This study showed that hypothermia in intestinal IR reduces inflammatory responses, ALI scores, and iNOS gene expression in lung tissues. There was no significant effect of hypothermia on the plasma NO concentration.

Keywords

References

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