Cancer Research and Treatment

The Korean Cancer Association (대한암학회)
권호 표지

Mouse Orthotopic Lung Cancer Model Induced by PC14PE6

Cui, Zheng-Yun;Ahn, Jin-Seok;Lee, Jee-Yun;Kim, Won-Seog;Lim, Ho-Yeong;Jeon, Hyun-Jung;Suh, SooWon;Kim, Jin-Hoon;Kong, Won-Ho;Kang, Ji-Min;Nam, Do-Hyun;Park, Keunchil

  • Published : 20060000
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Abstract

Purpose: This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells. Materials and Methods: Three cell doses (0.5⁓106; 1⁓106; 2⁓106) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells. Results: The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average lifespan of the three groups after inoculation was 14 days in the 2⁓106 cells inoculum group; 25 days in the 1⁓106 cells inoculum group; and 32 days in the 0.5⁓106 cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax. Conclusions: This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis. (Cancer Res Treat. 2006;38:234-239)

Keywords

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