Fractionation of Banaba Leaves Extract (Lagerstroemia speciosa L. Pers) and its Antioxidant Activity

바나바(Lagerstroemia speciosa L. Pars.) 잎 추출물의 분획 및 그들의 항산화 특성

Hwang, Ho-Sung;Lee, Yun-Kyung;Lee, Kwang-Geun
황호성;이윤경;이광근

  • Published : 20060000

Abstract

Dried Banaba Leaf (Lagerstroemia speciosa L. Pers.) has been traditionally used for treatment of diabetes as a folk medicine in the Philippines. Dried Banaba leaf contains corosolic acid which has a function as like insulin does. In this study, an antioxidant activity of Banaba leaves was measured. Dried Banaba leaves were pulverized and extracted by water, methanol, ethanol at above 80°C for 90 minutes. The extracts were tested for their antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity assay. The ethanol extract showed the highest antioxidant activity. The ethanol extract of Banaba leaf was fractionated using XA-2 resin and various solvents according to polarity. The antioxidant activity of each fraction was measured by lipidlMA (malonaldehyde) assay. Fraction I (the most polar) displayed the highest antioxidant activity.

Keywords

References

  1. 바나바(banaba ; Lagerstroemia speciosa Pers.). 식품원재료 데이터 베이스, 한국식품의약품안전청
  2. Dou, H., R. Zhang, X. Lou, J. Jia, C. Zhou and Y. Zhao. 2005. Constituents of three species of Lagerstroemia. Biochem. Syst. and Ecol. 33(6): 639-642 https://doi.org/10.1016/j.bse.2004.10.012
  3. Hattori1, K., N. Sukenobul, T. Sasaki1, S. Takasuga1, T. Hayashi, K. Ryoji, K. Yamasaki and O. Hazeki. 2003. Activation of insulin receptors by Lagerstroemin. J. Pharmacol Sci. 93: 69-73 https://doi.org/10.1254/jphs.93.69
  4. Hayashi, T., H., Maruyama, R., Kasai, K., Hattori, S., Takasuga, O,. Kazeki, K., Yamasaki and T., Tanaka. 2002. Ellagitannins from Lagerstroemia speciosa as activators of glucose transport in fat cells. Planta Med. 68: 173-175 https://doi.org/10.1055/s-2002-20251
  5. Horokazu, H., S. Akio, S. Yuko, S. Iwao and K. Takami. 2003. Isolation and quantitative analysis of the ${\alpha}-amylase$ inhibitor in Lagerstroemia speiosa (L.) Pers. (Banaba). The Pharmaceutical Society of Japan. 123(7): 599-605 https://doi.org/10.1248/yakushi.123.599
  6. Lee, K.G and T. Shibamoto. 2002. Determination of antioxidant potential of volatile extracts isolated from various herbs and spices. J. Agric. Food Chem. 50: 4947-4952 https://doi.org/10.1021/jf0255681
  7. Lee, K.G and T. Shibamoto. 2001. Antioxidant activities of volatile components isolated from Eucalyptus species. J. Sci. Food Agric. 81: 1573-1579 https://doi.org/10.1002/jsfa.980
  8. Liu, F., J.K. Kim, Y. Li, X.Q Liu, J. Li and X. Chen. 2001. An extract of Lagerstromia speciosa L. has insulin-like glucose uptake-stimulatory and adipocyte differentiationinhibitory activities in 3T3-L1 cells. American Society for Nutritional Sci. 01: 0022-3166
  9. Miura, T., Y. Itoh, T. Kaneko , N. Ueda , T. Ishida, M. Fukushima , F. Matsuyama and Y. Seino. 2004. Corosolic acid induces GLUT4 translocation in genetically type 2 diabetic mice. Biol. Pharm. Bull. 27(7): 1103-1105 https://doi.org/10.1248/bpb.27.1103
  10. Santos, A.C., L.F. Salatino Maria and A. Salatino. 1995. Flavonoids of species of cuphea(Lythraceae) from brazil. Biochem. Syst. Ecol. 23(1): 99-103 https://doi.org/10.1016/0305-1978(94)00071-N
  11. Sato, J., K. Goto, F. Nanjo and S. Kawai. 2000. Kousaku murata antijungal activity of plant extracts against Authrinium sacchari and Chaetomium funicola. J. Biosci. Bioeng.. 90(4): 442-446 https://doi.org/10.1016/S1389-1723(01)80016-5
  12. Takeo, H., M. Haruko, K. Royji, H. Katsuji, T. Shunsuke, K. Osamu, Y. Kazuo and T. Takashi. 2002. Ellagitannins from Lagerstroemia speciosa as activators of glucose transport in fat cells. Planta Med. 68: 173-175 https://doi.org/10.1055/s-2002-20251
  13. Umano, K., K.J. Dennis and T. Shibamoto. 1988. Analysis of free malonaldehyde in photoirradiated corn oil and beef fat via a pyrazole derivative. Lipids, 23: 811-814 https://doi.org/10.1007/BF02536226
  14. William, V.J., P.H. Siva, W.W. Stogsdill, S.J. Janet, M.A N. Ypusryand P. Richard. 2003. Antidiabetic activity of a standardized extract(GlucosolTM) from Lagerstroemia speciosa leaves in type II diabetics a dose-dependence study. J. Ethnopharmacol. 87: 115-117 https://doi.org/10.1016/S0378-8741(03)00122-3
  15. Yoshihito, O., O. Ayako, and O. Toru. 2003. A new triterpenoid isolated from Lagerstronemia speciosa (L.) Pers. Chem. Pharm. Bull. 51(4): 452-454 https://doi.org/10.1248/cpb.51.452