Quality of Rice Lines Transformed with Protox Gene of Bacillus subtilis

;;;Kuk, Yong In;Kim, Wook;Back, Kyoungwhan
김경문;구자옥;신말식

  • Published : 20060000

Abstract

Characteristics related to grain quality and starch viscosity were investigated in three independent japonica transgenicrice lines expressing a Protox gene from Bacillus subtilis and compared to the control. In this study, we found that there were nosignificant diferences between the transgenic lines and the control. All the transgenic lines were comparable in grain size, millingquality, appearance quality and physicochemical properties to the control that were derived from. The viscosity of the starch,breakdown values and setback value of the transgenic lines had similar to those of the control. In addition, textural properties ofposition of rice and properties of starch that determine the quality of rice and texture properties of cooked rice might provide usefulinformation for transgenic rice to rice breeder.

Keywords

References

  1. AACC 1983. Approved methods. 8th ed. American Association of Cereal Chemist, St. Paul, MN
  2. Andah A 1977. Comparative evaluation of different varieties of maize (com) for the manufacture of Kenkey (a Ghanaian fermented maize product). Food Research Institute Publication, Accra, Ghana
  3. AOAC 1990. Official Method of Analysis. 15th ed. Association of Official Analytical Chemists, Washington DC
  4. Bourne M 1978. Texture profile analysis. Food Technol. 32: 62-66
  5. Carrol RJ 1988. Effect of variations in parboiling on yield, color, and texture of recooked rice. MS thesis. University of Arkansas: Fayetteville
  6. Chrisou P 1997. Rice transformation: bombardment. Plant Mol. Biol. 35: 197-203 https://doi.org/10.1023/A:1005791230345
  7. Datta SK, Datta K, Soltanifar N, Donn G, Potrykus I 1992. Herbicide-resistant indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts. Plant Mol. Biol. 20:619-629 https://doi.org/10.1007/BF00046447
  8. Delwiche SR, McKenzie KS, Webb BD 1996. Quality characteristics in rice by near-infrared reflectance analysis of wholegrain milled samples. Cereal Chem. 73:257-263
  9. Gonzalez Z, Perez E 2002. Effect of acetylation on some properties of rice starch. Starch 54: 148-154 https://doi.org/10.1002/1521-379X(200204)54:3/4<148::AID-STAR148>3.0.CO;2-N
  10. Gibson TS, AI Qalla H, McCleary Bv\V 1992. An improved enzymatic method for the measurement of starch damage in wheat flour. J. Cereal Chem. 15: 15-27 https://doi.org/10.1016/S0733-5210(09)80053-2
  11. Hiei Y, Ohta S, Komari T, Kumashiro T 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 6:271-282 https://doi.org/10.1046/j.1365-313X.1994.6020271.x
  12. Juliano BO 1971. A simplified assay for miller-rice amylose. Cereal Sci. Today 16:334-340,360
  13. Jung S, Chung JS, Jang SM, Guh JO, Lee HJ, Chon SU, Kim K-M, Ha SB, Back K 2003. Either soluble or plastidic expression of recombinant protoporphyrinogen oxidase modulates tetrapyrrole biosynthesis and photosynthetic efficiency in transgenic rice. Biosci. Biotechnol. Biochem. 67: 1472-1478 https://doi.org/10.1271/bbb.67.1472
  14. Khush GS 1995. Modem varieties - their real contribution to food supply and equity. Geo J. 35:275-284
  15. Klein TM, Prentzen R, Lewis PA, Fitzpatrick-McElligott S 1992. Transformation of microbes, plants and animals by particle bombardment. Bio/Technol. 10:286-291 https://doi.org/10.1038/nbt0392-286
  16. Kuk YI, Lee HJ, Chung JS, Kim KM, Lee SB, Ha SB, Back K, Guh JO 2005. Expression of a Bacillus subtiils protoporphyrinogen oxidase gene in rice plants reduces sensitivity to peroxidizing herbicides. Biol. Plant. 49:577-583 https://doi.org/10.1007/s10535-005-0052-3
  17. Lee HJ, Lee SB, Chung JS, Han SU, Han O, Guh JO, Heo JS, An G, Back K 2000. Transgenic rice plants expressing a Bacillus subtilis protoporphyrinogen oxidase gene are resistant to diphenyl ether herbicide oxyfluofen. Plant Cell Physiol. 41: 743-749 https://doi.org/10.1093/pcp/41.6.743
  18. Lee SY, Pyun YR, Cho HY, Yu JH, Lee SK 1984. Flow behaviors of native and gelatinized rice starch solutions. Kor. J. Food Technol. 16:29-36
  19. Martinez-Herrera ML, LaChance PA 1979. Com (Zea mays) kernel hardiness as index the alkaline cooking time for tortilla preparation. J. Food Sci. 44:377-380 https://doi.org/10.1111/j.1365-2621.1979.tb03793.x
  20. Medcalf DC, Gilles KA 1965. Wheat starch. I. Comparison of physicochemical properties. Cereal Chem. 42:558-568
  21. Monsoor MA, Proctor A, Siebenmorgen TJ 2004. Surface lipid and FFA content of head and broken rice produced by milling after different drying treatments. Cereal Chem. 81: 705-709 https://doi.org/10.1094/CCHEM.2004.81.6.705
  22. Montgomery EM, Senti FR 1958. Separation of amylose from amylopectin of starch by an extraction-sedimentation procedure. J. Polymer Sci. 28: 1-3 https://doi.org/10.1002/pol.1958.1202811601
  23. Pauli H 1976. Proposed extension of the CIE recommendation on 'Uniform color spaces, color difference equations, and metric color terms'. J. Opt. Soc. Am. 66:866-867 https://doi.org/10.1364/JOSA.66.000866
  24. Ross AS, Quail KJ, Crosbie GB 1997. Physicochemical properties of Australian flours influencing the texture of yellow alkaline noodles. Cereal Chem. 74:814-820 https://doi.org/10.1094/CCHEM.1997.74.6.814
  25. Schoch TJ 1964. Swelling power and solubility of granular starches, p.106-108. In: RL Whistler (ed.). Methods in Carbohydrate Chemistry (Vol. 4). Academic Press, NY
  26. Szceniak AS 1963. Classification of textural characteristics. J. Food Sci. 28:385-389 https://doi.org/10.1111/j.1365-2621.1963.tb00215.x
  27. Tester RF, Morrison WR 1990. Swelling and gelatinization of cereal starches. I. Effects of amylopectin, amylose and lipids. Cereal Chem. 67:551-557
  28. Webb BD 1991. Rice quality and grades, p. 94-104. In: BS Luh (ed.). Rice (Vol. 2): Utilization. Van Nostrand Reinold, New York
  29. Williams PC, Kuzina FD, Hlynka I 1970. A rapid colorimetric procedure for estimation the amylose content of starches and flours. Cereal Chem. 47:411-420