Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Microspore-derived Embryogenesis and Plant Regeneration from Anther Culture of Pulsatilla cernua var. koreana

할미꽃 약배양에 의한 소포자 유래 배 발생 및 식물체 재분화

Ko, Jeong-Aae;Kim, Hyun-Sun
고정애;김현순

  • Published : 20060000
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Abstract

Microspores of Pulsatilla cernua var. koreana (Ranunculaceae) which could produce embryogenic calli or embryos were cultured on Murashige and Skoog’s medium with different kinds of plant growth regulators. Three different pathways could be recognized in this process; the first involving the main vegetative cells, the second containing all the generative and vegetative cells, and the third starting with two equal cells in the pollen grains. Androgenesis initiation was highest when anthers contained pollen at the uninucleate stage or early binucleate stage 4-5 days before anthesis. MS medium with 0.5 mgL-1 NAA and 1.0 mgL-1 BA was found to be highly desirable for the induction of androgenesis in this plants. Addition of 10% coconut water to the culture medium seemed to significantly improve callus or embryo production. The calli developed buds and subsequently regenerated plantlets. Shoot multiplication and elongation were also increased by adding 10% coconut water. Rooting occurred rarely from the plantlets that formed from the pollen embryos. However, 5% of roots from shoot were produced on MS medium with 0.5 mgL-1 NAA and 1.0 mgL-1 BA.

MS 배지에 식물생장조절물질을 첨가하여 할미꽃 약배양을 실시한 결과 소포자 유래 캘러스 및 배가 형성되었다. 약내 소포자는 첫째, 영양세포, 둘째, 생식 및 영양세포 모두, 셋째, 균등 분열된 두 영양세포들이 배를 형성하는 3가지 경로가 확인되었다. 소포자유래 배형성에 가장 적합한 시기는 개화 4-5일전의 1핵성 소포자기 또는 초기 2핵성 화분 상태였다. 0.5mgL-1 NAA와 1.0mgL-1 BA가 혼용처리된 MS 배지가 할미꽃 약배양에 적합하였으며, 10% coconut water를 첨가했을 때 소포자 유래 캘러스, 배형성 및 신초수와 초장이 증가되었다. 소포자 배 유래 재분화 식물체는 뿌리 분화가 늦었으나 0.5mgL-1 NAA와 1.0mgL-1 BA가 첨가된 MS 발근배지에서 5% 정도 뿌리 분화를 보여 효과적이었다.

Keywords

References

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