Current Applied Physics

Korean Physical Society (한국물리학회)
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A new method for immobilization of lipid-binding proteins to the glass slides

Shin, Jaeyoung;Cho, Minho;Hyun, June Won;Pyee, Jaeho;Park, Heonyong

  • Published : 20060000
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Abstract

Previously, we constructed nickel-coated glass slides to immobilize histidine-tagged proteins [Bull. Korean Chem. Soc. 23 (2002)17241728]. Here, we further investigated whether the nickel-coated glass slide could be utilized for assaying molecular interactionbetween lipid and immobilized protein. Proteins interact with various lipid molecules in biological systems. More importantly, thisinteraction is responsible for a broad spectrum of physiological functions. Accordingly, to more eciently analyze various types ofthe high-throughput analytical purpose, we immobilized an FITC (uorescein isothiocyanate)-tagged lipid-binding protein tonickel-coated plates, incubated the plates with various lipid molecules and then measured uorescence intensities to analyze its bind-ing anity. Our systems seemed to be reliable for the application to the high-throughput system consisted of lipid-binding proteins.

Keywords

References

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