Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Random Amplified Polymorphic DNA Analysis for Origin Identification of Olive Flounder (Paralichthys olivaceus) and Redlip Croaker (Pseudosciaena polyactis)

넙치와 조기의 원산지 판별을 위한 random amplified polymorphic DNA 패턴 연구

  • Kang Duk-Jin (Department of Chemistry and East Coastal Marine Bioresources Research Center) ;
  • Lee Suk-Keun (Department of Oral Pathology, Kangnung National University) ;
  • Jin Deuk-Hee (Faculty of Marine Bioscience & Technology, Kangnung National University) ;
  • Choi Suk-Jung (Department of Chemistry and East Coastal Marine Bioresources Research Center)
  • Published : 2006.02.01
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Abstract

The random amplified polymorphic DNA (RAPD) technique was investigated as a potential tool for the origin identification of olive flounder (Paralichthys olivaceus) and redlip croaker (Pseudosciaena polyactis). Olive flounder specimens were collected from North Korea and several locations of South Korea (Jumunjin, Tongyoung and Geoje). Fishes obtained from Tongyeong and Geoje were cultured products. Redlip croaker specimens were collected from South Korea and China. Consistent and distinct diagnostic bands were easily identified in the RAPD patterns of the olive flounder specimens. Although consistent diagnostic bands rarely appeared in the RAPD pattern of redlip croaker specimens because of their genetic heterogeneity, we were able to find potential diagnostic bands in the average RAPD pattern of each origin.

본 연구에서 넙치와 조기의 원산지를 판별하기 위한 도구로 RAPD PCR 방법의 가능성을 확인하였다. 넙치는 한국의 주문진 자연산, 통영 양식산, 거제 양식산 그리고 북한 자연산을 실험에 사용하였다. 조기는 한국산과 중국산을 사용하였다. 넙치의 RAPD 패턴에서는 뚜렷하고 일관성이 있는 진단용 띠들을 쉽게 찾을 수 있었다. 조기의 경우에는 유전적인 이질성으로 인하여 각 개체의 RAPD 패턴에서는 일관성이 있는 진단용 띠를 찾기 어려웠지만 각 원산지별로 얻은 RAPD 패턴에서는 가능성이 있는 진단용 띠들을 찾을 수 있었다.

Keywords

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