Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Studies on the Anti-inflammatory Activity of Paulownia coreana Uyeki Leaf Extract

오동나무 잎 추출물의 항염 효능에 관한 연구

  • Kim, Nam-Kyoung (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town) ;
  • Kim, Mi-Hwa (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town) ;
  • Yoon, Chang-Soon (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town) ;
  • Choi, Shin-Wook (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town)
  • Published : 2006.12.30
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Abstract

This work was carried out to investigate the anti-inflammatory effects of Paulownia coreana Uyeki on abirritant, atopy and acne skin. Paulownia coreana Uyeki has been used as a traditional medicine having anti-febrile, anti-inflammation effect in Korea, Paulownia coreana Uyeki loaves were extracted with 70% EtOH. Its superoxide anion radical scavenging activity and inhibitory effect on LPS-induced NO production were examined. The extract inhibitied the generation of NO and $PGE_2$ induced by LPS in the macrophage cell line (Raw 264.7). Consistent with the inhibitory effects on No and $PGE_2$ generation, the extract inhibited expression of iNOS and COX-2. In further study, it was found that the extract prevented $IkB-{\alpha}$ degradation, as demonstrated by western blot analysis of $IkB-{\alpha}$ protein level. However, the extract treatment did not affect cell viability at $100{\mu}g/mL$ concentration in both human skin fibroblast and Raw 264.7 cells in vitro. Thus, the present study suggests that Paulownia coreana Uyeki leaves extract have significant anti-inflammatory activity and potential as an anti-irritation material.

본 연구는 피부에 안전하고 부작용이 없는 식물성 원료를 이용한 항염 소재를 개발하여 피부 진정 및 여드름, 아토피 피부에 적용되어질 수 있는 피부 자극 완화제를 개발하고자 하였다. 이에 따라 전통적으로 염증 질환 치료에 사용되어진 오동나무(Paulownia coreana Uyeki)를 소재로 하여 오동나무 잎 추출물의 항산화 효능과 항염 효능을 알아봄으로써 화장품 원료로의 가능성을 확인하였다. EPR을 이용하여 오동나무 잎 추출물의 superoxide anion radical 소거능을 확인하였으며 대식세포주에서의 LPS 처리에 의해 유도되어지는 NO의 생성을 저해시키는 효능을 확인하였다. 그 결과 추출물의 우수한 NO 생성 저해능을 확인하여 오동나무 잎 추출물의 항염 효능 메카니즘을 규명하였다. ELISA 기법을 이용하여 $PGE_2$ 생성 억제 효능을 확인하였으며 NO와 $PGE_2$ 생성 단백질인 iNOS와 COX-2의 발현 여부를 확인한 결과 오동나무 잎 추출물 처리에 의해 두 단백질의 발현이 확연히 감소되는 것을 확인하였다. 또한 염증 반응의 초기 단계에 관여하는 $IkB-{\alpha}$ 단백질의 분해정도를 시간별로 확인한 결과 LPS 처리 30 min 후 $IkB-{\alpha}$ 단백질의 분해가 일어나게 되는데 오동나무 잎 추출물을 함께 처리하였을 경우 시간이 경과되어도 $IkB-{\alpha}$ 단백질의 분해가 일어나지 않는 것을 확인하였다. 이로써 오동나무 잎 추출물의 항염 효능은 염증 반응 신호 전달의 초기 단계에 해당하는 $IkB-{\alpha}$ 단백질의 분해를 억제함으로써 결과적으로 NO와 $PGE_2$의 생성을 감소시킴을 확인하였다. 또한 CCD-986sk와 Raw 264.7 세포에 대한 세포 독성이 존재하지 않음을 확인함으로써 오동나무 잎의 항염 효능을 이용한 피부 자극완화제로서의 개발 가능성을 확인하였다.

Keywords

References

  1. C. L. Si, J. K Kim, D. J. K won, and Y. S. Bae, Phenolic compounds from the fruits of Paulownia coreana Uyeki. Mokchae Konghak, 34, 79 (2000)
  2. J. S. Oh, H. I. Moon, and O. P. Zee, Cytotoxic compounds from the flowers of Paulownia coreana, Kor. J. Pharmacogn., 31, 449 (2000)
  3. S. Damtoft and S. R. Jensen, Tomentoside and 7-hydroxytomentoside, two iridoid glucosides from Paulownia tomentosa, Phytochemistry, 34, 1636 (1993) https://doi.org/10.1016/S0031-9422(00)90861-6
  4. 사전령, 김진규, 권동주, 배영수, 오동나무 잎의 페놀성 화합물, 산림과학연구, 21, 23 (2005)
  5. K S. Park, H. J. Kim, E. J. Kim, K. T. Nam, J. H. Oh, C. W. Song, H. K. Jung, D. J. Kim, Y. W. Yun, H. S. Kim, S. Y. Chung, D. H. Cho, B. Y. Kim, and J. T. Hong, Effect of glycolic acid on UVB-induced skin damage and inflammation in guinea pigs, Skin Pharmacol Appl. Skin Physiol., Jul-Aug, 15, 236 (2002) https://doi.org/10.1159/000065970
  6. S. Kang, E. A. Duell, G. J. Fisher, S. C. Datta, Z. Q. Wang, A. P. Reddy, A Tavakkol, J. Y. Yi, C. E. Griffiths, and J. T. Elder, Application of retinol to human skin in vivo induces epidermal hyperplasia and cellular retinoid binding proteins characteristic of retinoic acid but without measurable retinoic acid levels or irritation, J. Invest. Dermatol. Oct., 105, 549 (1995) https://doi.org/10.1111/1523-1747.ep12323445
  7. O. M. Czarnecka, W. M. Bator, and W. Silny, Atopy patch test reaction to airborne allergens in the diagnosis of atopi dermatitis, Acta Dermatovenerol. Croat., 13, 3 (2005)
  8. Y. F. Leung, P. O. Tam, W. S. Lee, D. S. Lam, H. F. Yam, B. J. Fan, C. C. Tham, J. K. Chua, and C. P. Pang, The dual role of dexamethasone on anti -inflarnmation and outflow resistance demonstrated in cultured human trabecular meshwork cells, Mol. Vis. Sep., 59, 425 (2003)
  9. D. Rocksen, B. Lilliehook, R. Larsson, T. Johansson, and A Bucht, Differential anti -inflamrnatory and anti-oxidative effects of dexamethasone and Nacetylc-ysteine in endotoxin-induced lung inflammation, Clin. Exp. Immunol., 122, 249 (2000) https://doi.org/10.1046/j.1365-2249.2000.01373.x
  10. Y. Endo, S. Tsuriya, and E. Fujihira, Hepatic drugmetabolizing enzyme activities and anti-inflammatory potency of hydrocortisone in rats with granulomatous inflammation, Res. Commun Chem Pathol. Pharmacol., 33, 195 (1981)
  11. C. B. Favour, Local anti-inflammatory effect of hydrocortisone, Stanford Med. Bull., 6, 15 (1958)
  12. S. Sato and K. Maehara, Anti -inflarnmatory and analgesic effects of benzy-damine on thermal inflammation, J. Osaka. Dent. Univ., 1, 37 (1967)
  13. S. M. Han, K. G. Lee, J. H. Yea, and H. Y. K wean, Anti -inflammatory effect of the vanom from asian honeybees (Apis cerana L.) on inducible nitric oxide synthase and tumor necrosis factor-$\alpha$ in Raw 264.7 cell line, Korean J. Apiculture, 19, 89 (2004)
  14. E. S. Lee, H. K. Ju, T. C. Moon, E. Lee, Y. Jahng, S. H. Lee, J. K. Son, S. H. Baek, and H. W. Chang, Inhibition of nitric oxide and tumor necrosis factor-alpha (TNF -alpha) production by propenone compound through blockade of nuclear factor (NF)kappa B activation in cultured murine macrophages, Biol. Pharm. Bull., 27, 617 (2004) https://doi.org/10.1248/bpb.27.617
  15. Y. C. Liang, Y. T. Huang, S. H. Tsai, and S. Y. Lin-Shiau, Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages, Carcinogenesis, 20, 1945 (1999) https://doi.org/10.1093/carcin/20.10.1945
  16. H. M. Shin, M. H. Kim, B. H. Kim, S. H. lung, Y. S. Kim, H. J. Park, J. T. Hong, K R. Min, and Y. Kim, Inhibitory action of novel aromatic diamine compound on lipopolysaccharide induced nuclear translocation of NF-kappaB without affecting I kappaB degradation, FEBS, 30, 50 (2004)