Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effect of Antioxidation and Inhibition of Matrix Metalloproteinase-1 from Ligularia fischeri

곰취의 항산화와 UVA에 의한 MMP-1 발현 저해효과

  • 나영 (한불화장품(주) 기술연구소) ;
  • 김진화 (한불화장품(주) 기술연구소) ;
  • 심관섭 (한불화장품(주) 기술연구소) ;
  • 이범천 (한불화장품(주) 기술연구소) ;
  • 표형배 (한불화장품(주) 기술연구소)
  • Published : 2006.09.30
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Abstract

In this study, we examined the effects of antioxidant, lipid peroxidation inhibition and suppression of UVA-induced MMP-1 expression in human dermal fibroblasts. Ligularia fischeri extract showed free radical scavenging effect by 82.3% at 5 mg/mL and superoxide radical scavenging effect by 79.3% at 5 mg/mL in the kanthine/xanthine oxidase system, respectively. At the concentration of $500{\mu}g/mL$, L. fischeri extract showed 97% inhibition on lipid peroxidation of linoleic acid. UVA induced MMP expression in human dermal fibroblasts was reduced 35% by treatment with $100{\mu}g/mL$ of L. fischeri extract. These results suggest that L. fischeri extract can be used for an anti-aging agent by antioxidation, lipid peroxidation inhibition and reducing UVA-induced MMP-1 production.

본 연구는 곰취 추출물의 항산화, 지질과산화 억제 및 자외선 조사에 의해 유도된 MMP-1 발현에 대한 영향을 사람 섬유아세포를 이용하여 확인하였다 곰취의 DPPH와 superoxide radical 소거효과는 처리농도가 증가함에 따라 농도 의존적으로 소거효과를 나타냈으며, 각각 5mg/mL에서 82.3%와 79.3%로 DPPH와 superoxide radical을 소거하여 우수한 항산화 효과를 나타내었다. 곰취 추출물의 지질과산화 억제효과는 $500{\mu}g/mL$에서 97.0%로 지질과산화 억제효과도 우수하게 나타났다. 또한 자외선에 의해 증가된 사람 섬유아세포의 MMP-1 단백질 발현양은 곰취 추출물을 $100{\mu}g/mL$ 농도를 처리함으로써 약 35%로 감소되었다. 곰취 추출물은 항산화 효과, 지질과산화 억제효과 및 자외선 조사에 의해 유도되는 MMP-1 단백질 발현량을 조절하는 기능을 갖는 것을 확인하였다. 따라서 곰취는 항산화, 지질과산화 억제 및 자외선에 의해 유도되는 MMP-1 발현을 저해함으로써 항노화 소재로써 이용될 수 있을 것으로 사료된다.

Keywords

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