Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effects of Lignan Compound of Sesame on LPS-induced Nitric Oxide Generation in Murine Macrophage RAW 264.7 Cells

참깨의 리그난 화합물의 항염증 효과

  • Published : 2006.09.30
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Abstract

Sesame (Sesamum indicum L.), one of the oldest oilseed crops, has been known to posses antioxidative and inflammatory effects. This seed contains lignan compounds such as sesamin, sesamol, sesaminol, sesaminol diglucosides (SDG), and sesaminol triglucosides (STG). Sesamin, a major lignan in sesame, displayed several biological activities including a protective effects against oxidative damage in the skin. In the present study, we investigated the effect of sesamin, sesamol, sesaminol, SDG, and STG, on nitric oxide (NO) induction and inducible nitric oxide synthane (iNOS) and cyclooxygenases-2 (COX-2) expression in lipopolysaccharides (LPS)-treated RAW 264.7 cells. The results showed that sesamol and sesaminol significantly inhibited NO generation but they were also cytotoxicity however, sesamin effectively inhibited NO production ($IC_{50}: 64{\mu}M$) without my cytotoxic effect in LPS-stimulated macrophage RAW 264.7 cells. In further study, it was founded that sesamin inhibited the expression of inducible nitric oxide synthase but not COX-2 expression. These results suggest that sesamin may be useful for improvements of the inflammatory diseases.

참깨(Sesamum indicum L.)는 1년생 초본식물로서 볶은 참깨나 참기름은 우리나라 식단에서 아주 중요한 조미용 양념 및 식용유지로서 이용되고 있다. 약효로는 동맥경화, 고혈압 및 노화 예방 등으로 알려져 왔으나, 참깨에 함유되어 있는 어떤 성분이, 이들 약효에 관여하고 있는지의 과학적인 규명이 미흡한 실정이다. 본 연구에서는 천연물 유래의 새로운 노화방지제를 개발하기 위해 참깨의 리그난 화합물(sesamin, sesamol, sesaminol, sesaminol diglucosides (SDG), sesaminol triglucosides (STG))의 항염증 활성을 검색하고, 그 중에서 항염증 효과가 가장 높게 나타난 sesamin을 전처치 방법과 동시처치 방법으로 NO 생성억제효과, 세포독성, inducible nitric oxide synthase (iNOS) 및 cyclooxygenases-2 (COX-2) 발현에 미치는 영향을 탐색하고 작용기작을 규명하고자 하였다. 리그난 화합물의 NO 생성억제효과를 탐색한 결과, sesamol, sesaminol, sesamin에서 NO 생성에 미치는 억제율이 높았으나, sesamol, sesaminol은 세포독성이 나타났다. 하지만 sesamin ($IC_{50}: 64{\mu}M$)은 세포독성은 없으면서 농도의존적으로 NO 생성을 억제시켰으며, iNOS 발현에서도 억제 효과를 보여주었다. 결론적으로 sesamin은 피부노화에 원인 중 하나이며 체내 염증반응의 중요 매개인자인 NO의 생성을 억제하는 항염증 물질로서 이와 같은 연구 결과는 향후 임상에서 항염증과 관련된 새로운 항노화 전략으로 개발하기 위해 기초자료로 응응될 수 있음을 시사한다.

Keywords

References

  1. 김금숙, 김동휘, 정미란, 장인복, 심강보, 강철환, 이승은, 성낙술, 송경식, 참깨품종별 Sesamin, Sesamolin 함량 변이, korean J. Crop Sci., 49, 496 (2004)
  2. 엄민영, 최원희, 안지윤, 김성란, 하태열, 노화촉진마우스에 있어서 참깨박 및 들깨박의 메탄올 추출물이 인지기능 및 항산화능에 미치는 영향, 한국식품학회지, 4, 637 (2004)
  3. 강명화, 오명규, 방진기, 김동휘, 강철환, 이봉호, 국내 참깨 품종의 리그난 함량 및 지방산 조성, Korean J. Crop Sci., 45, 203 (2000)
  4. W. H. Wu, Y. P. Kang, N. H. Wang, H. J. Jou, and T. A. Wang, Sesame ingestion affecta sex hormones, antioxidant status, and blood lipids in postmenopausal women, J. Nutr., 136, 1270 (2006) https://doi.org/10.1093/jn/136.5.1270
  5. D. Nakano, C. J. Kwak, K Fujii, K. Ikemura, A. Satake, M. Ohkita, M. Takaoka, Y. Ono, M. Nakai, N. Tomimori, Y. Kiso, and Y. Matsumura, Sesarnin metabolites induce an endothelial nitric oxide dependent vasorelaxation through their antioxidative property-independent mechanisms: possible involvement of the metabolites in the antihypertensive effect of sesarnin, J. Pharmacol. Exp. Ther., 318, 328 (2003) https://doi.org/10.1124/jpet.105.100149
  6. D. Z. Hsu, S. B. Su, S. P. Chien, P. J. Chiang, Y. H. Li, Y. J. Lo, and M. Y. Liu, Effect of sesame oil on oxidative-stress-associated renal injury in endotoxernic rats: involvement of nitric oxide and proinflammatory cytokines, Shock, 24, 276 (2005) https://doi.org/10.1097/01.shk.0000172366.73881.c7
  7. Y. Kiso, N. Tsuruoka, A. Kidokoro, I. Matsumoto, and K. Abe, Sesarnin ingestion regulates the transcription levels of hepatic metabolizing enzymes for alcohol and lipids in rats, Alcohol Clin. Exp. Res., 29, 116S (2005) https://doi.org/10.1097/01.alc.0000189296.99704.1f
  8. L. C. Mullany, G. L. Darmstadt, S. K. Khatry, and J. M. Titlsch, Traditional massage of newborns in Nepal: implications for trials of improved practice, J. Trop. Pediatr., 51, 82 (2005) https://doi.org/10.1093/tropej/fmh083
  9. 박찬국, 한의학 특강, 집문당 (2002)
  10. 김이현, 한방피부미용법 330 지혜, 한방미디어 (2000)
  11. H. Kawamata, H. Ochiai, N. Mantani, and K. Terasawa, Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line, Am J. Chin Med., 28, 217 (2000) https://doi.org/10.1142/S0192415X0000026X
  12. B. G. Lee, S. H. Kim, O. P. Zee, H. Y. Lee, J. W. Han, and H. W. Lee, Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach, Eur. J. Pharmacol., 406, 301 (2004) https://doi.org/10.1016/S0014-2999(00)00680-4
  13. W. G. Sea, H. O. Pae, G. S. Oh, K. Y. Chai, Y. G. Yun, T. O. Kwon, and H. T. Chung, Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide, Gen Pharmacol., 35, 21 (2000) https://doi.org/10.1016/S0306-3623(01)00086-6
  14. W. F. Chiou, C. J. Chou, and C. F. Chen, Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages, Life. Sci., 69, 625 (2001) https://doi.org/10.1016/S0024-3205(01)01154-7
  15. H. J. Park, S. H. Lee, D. J. Son, K. W. Oh, K. H. Kim, H. S. Song, G. J. Kim, G. T. Oh, D. Y. Toon, and J. T. Hong, Antiarthritic effect of bee venom: Inhibition of inflammation mediator generation by suppression of NF- $\kappa$B through interaction with the p50 Subunit, Arthritis Rheum., 50, 3504 (2004) https://doi.org/10.1002/art.20626
  16. X. Mariette, Tolerance of anti-TNF alpha: the experience acquired in inflammatory rheumatic disease, Ann Dermatol Venereol., 133, 158 (2006)
  17. H. S. Jang, S. K. Kim, J. B. Han, H. J. Ahn, H. Bae, and B. I. Min, Effects of bee venom on the pro-inflammatory responses in RAW 264.7 macrophage cell line, J. Ethnopharmcol., 13, 157 (2005) https://doi.org/10.1016/0378-8741(85)90003-0
  18. D. Z. Hsu, K. T. Chen, Y. H. Li, Y. C. Chuang, and M. Y. Liu, Sesamol delays mortality and attenuates hepatic injury after cecal ligation and puncture in rats: role of oxidative stress, Shock, 25, 528 (2006) https://doi.org/10.1097/01.shk.0000209552.95839.43