Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Genetic Variation in the Natural Populations of Korean Stewartia (Stewartia koreana Nakai) Based on I-SSR Analysis

I-SSR 분석에 의한 노각나무 천연집단의 유전변이

  • Yang Byeung-Hoon (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Koo Yeong-Bon (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Park Yong-Goo (Department of Forestry, Kyungpook National University) ;
  • Han Sang-Don (Division of Forest Genetic Resources, Korea Forest Research Institute)
  • 양병훈 (국립산림과학원 산림유전자원부) ;
  • 구영본 (국립산림과학원 산림유전자원부) ;
  • 박용구 (경북대학교 임학과) ;
  • 한상돈 (국립산림과학원 산림유전자원부)
  • Published : 2006.02.01
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Abstract

We investigated the genetic variation in Stewartia koreana Nakai by examining 61 I-SSR amplicons in 120 individuals distributed among six natural populations in Korea. The overall percentage of polymorphic I-SSR amplicons was 81.9% and mean number of amplicons per I-SSR primer was 12.2. Levels of genetic diversity within 6 populations were similar each other[Shannon's Index $0.358{\sim}0.467$(mean: 0.407)]. The Mt. Obong population had the highest level of genetic diversity and was most distinctive from the other populations. Most variation existed among individuals within population(88.2%). Genetic differentiation among populations(${\phi}_{ST}$) was 0.118. The UPGMA dendrogram based on the genetic distance failed in showing decisive geographic relationships.

본 연구는 우리나라 특산수종이며 조경 및 원예적 가치가 높은 노각나무 유전변이를 조사하기 위해 6개 천연집단을 선발하여 DNA I-SSR 표지자를 사용, 유전다양성 및 유전구조를 조사하였다. 5개의 I-SSR primer(#813, 815, 818, 820, 823)에서 총 61개의 증폭산물을 관찰할 수 있었으며, 유전 다양성을 나타내는 P(Percentage of polymorphic loci)값과S.I.(Shannon's information Index)가 남쪽에 분포하는 오봉산(P=88.5%, S.I.=0.467), 금산(P=86.9%, S.I.=0.427), 바랑산(P=83.6%, S.I.=0.425)집단이 높았으며, 북쪽(내륙)에 분포하는 소백산(P=80.3%, S.I.=0.396), 지리산(P=77.1%, S.I.=0.368), 가야산(P=75.4%, S.I.=0.358)집단은 낮았다. 전체 유전변이 중 11.8%만이 집단간에 기인하는 것으로 나타났고, 나머지 88.2%는 집단내 개체간의 차이에서 기인하였다. 유전거리를 이용하여 UPGMA법에 의한 유집분석을 실시한 결과 지리적 분포에 대한 뚜렷한 경향은 나타나지 않았다.

Keywords

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