Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bacterial Community Structure in Activated Sludge Reactors Treating Free or Metal-Complexed Cyanides

  • Quan Zhe-Xue (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University) ;
  • Rhee Sung-Keun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Department of Microbiology, Chungbuk National University) ;
  • Bae Jin-Woo (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Baek Jong-Hwan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Park Yong-Ha (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Sung-Taik (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • Published : 2006.02.01
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Abstract

The microbial activity and bacterial community structure of activated sludge reactors, which treated free cyanide (FC), zinc-complexed cyanide (ZC), or nickel-complexed cyanide (NC), were studied. The three reactors (designated as re-FC, re-ZC, and re-NC) were operated for 50 days with a stepwise decrease of hydraulic retention time. In the re-FC and re-ZC reactors, FC or ZC was almost completely removed, whereas approximately 80-87% of NC was removed in re-NC. This result might be attributed to the high toxicity of nickel released after degradation of NC. In the batch test, the sludges taken from re-FC and re-ZC completely degraded FC, ZC, and NC, whereas the sludge from re-NC degraded only NC. Although re-FC and re-ZC showed similar properties in regard to cyanide degradation, denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene of the bacterial communities in the three reactors showed that bacterial community was specifically acclimated to each reactor. We found several bacterial sequences in DGGE bands that showed high similarity to known cyanide-degrading bacteria such as Klebsiella spp., Acidovorax spp., and Achromobacter xylosoxidans. Flocforming microorganism might also be one of the major microorganisms, since many sequences related to Zoogloea, Microbacterium, and phylum TM7 were detected in all the reactors.

Keywords

References

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