Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Selection and Characterization of Peptides Specifically Binding to $TiO_2$ Nanoparticles

  • Seo Min-Hee (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie) ;
  • Lee Jong-Ho (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie) ;
  • Kim Min-Soo (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie) ;
  • Chae Hee-K. (Department of Chemistry Education, Seoul National University) ;
  • Myung Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie)
  • Published : 2006.02.01
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Abstract

We have screened phage display peptide libraries to select for peptides binding to various sized $TiO_2$ nanoparticles. Phage libraries displaying random 7mer, 12mer, and C-7-Cmer peptides were used for screening. The size of target $TiO_2$ particles used were 7 nm, 15 nm, and 25 nm in diameter. We could select peptides binding each nanoparticles from all 3 libraries. Their binding was confirmed by transmission electron microscopy (TEM). Each peptide investigated was also shown to bind the other sized particles, meaning that the binding was specific for the nature of the particle rather than for the size of it. One of the 7mer peptides (PEP9, SVSPISH) was chosen for further analysis. The binding was shown to be in a dose-dependent manner, suggesting a specific interaction.

Keywords

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