Impact Analysis of Neem Kernel Extracts on the Developmental Profile of Anopheles stephensi

  • Sharma, Preeti (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute(Deemed University)) ;
  • Mohan, Lalit (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute(Deemed University)) ;
  • Srivastava, C.N. (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute(Deemed University))
  • Published : 2006.03.01

Abstract

Laboratory evaluation of the larvicidal efficacy of Ageratum conyzoides, Argemone mexicana and Azadirachta indica was carned out against Anopheles stephensi to develop an effective ecofriendly insecticide of plant origin. The methanol extract of A. indica was observed remarkably active with $LC_{50}$ values 18.2 and 13.1 ppm after 24 and 48 hours of exposure. Petroleum ether extracts of Ag. conyzoides with $LC_{50}$ 425.6 and 267.9 ppm, carbon tetra chloride extract of Ar. mexicana with $LC_{50}$ 313.2 and 240.3 ppm and petroleum ether extract of Az. indica with $LC_{50}$ 189.1 and 53. 5 ppm after 24 and 48 hours of exposure were also found effective. The impact of Az. indica methanol extract on the life cycle of malaria vector was further observed. Methanol extract of Az. indica causes deformities in different developmental stages of the malaria vector. Total developmental period was also extended from 10 to 15 It remarkably influenced hatching rates, larval-pupal transformation and development, adult emergence and growth index. Larval and pupal periods were also significantly prolonged.

Keywords

References

  1. Awala, P., R.W. Mwangi and L.W. Irungu. 1998. Larvicidal activity of Melia volkensii against Aedes aegypti. Insect Sci. Applic. 18: 225-228
  2. Bhagat, O.P. 1992. Morphogenetic aberration and growth regulatory effect of Ocimum sanctum against Anopheles stephensi. Biomedicine. 3: 153-156
  3. Finney, D.J. 1971. Probit Analysis. Third edition Cambridge University Press, Cambridge
  4. Gericke, A., J.M. Govere and D.N. Durrheim. 2002. Insecticide susceptibility in the South African malaria mosquito Anopheles arabiensis (Diptera: Culicidae). South African J. Sci. 98: 1-4
  5. Hargreaves, K., L.L. Koekemoer, B.D. Brooke, R.H. Hunt, J. Mthembu and M. Coetzee. 2000. Anopheles funestus resistant to pyrethroid insecticides in South America. Med. Vet. Entomol. 14: 181-189 https://doi.org/10.1046/j.1365-2915.2000.00234.x
  6. Jarial, M.S. 2001. Toxic effect of garlic extracts on the eggs of Aedes aegypti. J. Med. Entomol. 38: 446-450 https://doi.org/10.1603/0022-2585-38.3.446
  7. Mehra, B.K. 1998. Plant extract induced developemental deformities in Culex quinquefasciatus (Diptera: Culicidae). Indian J. Appl. Entomol. 12: 45-50
  8. Mohan, L., P. Sharma, C.N. Srivastava. 2005. Evaluation of Solanum xanthocarpum extracts as mosquito larvicides. J. Environ. Biol. 26: 399-401
  9. Murugan, K. and D. Jeyabelan. 1999. Effect of certain plant extracts against the Mosquito, Anopheles stephensi Liston. Curr. Sci. 76: 631-633
  10. Park Ross, G.A. 1936. Insecticide as a major measure in the control of malaria, being an account of the methods and organizations put into force in Natal and Zululand during the past six years. Quarterly Bulletin of the Health Organization of the League of Nations. 5: 114-133
  11. Rajkumar, S. and A. Jebanesan. 2004. Mosquitocidal activities of octacosane from Moschosma polystachyum Linn, (lamiaceae). J. Ethnopharmacol. 90: 87-89 https://doi.org/10.1016/j.jep.2003.09.030
  12. Saxena, A. and R.C. Saxena. 1992. Effect of Ageratum cony-zoides extract on the developmental stages of malaria vector, Anopheles stephensi (Diptera: Culicidae). J. Environ. Biol. 13: 207-209
  13. Saxena, S.C. and R.S. Yadav. 1983. A new plant extract to suppress the population of yellow fever and dengue vector Aedes aegypti (Diptera: Culicidae). Curr. Sci. 52: 713-715
  14. Sharma, P., L. Mohan and C.N. Srivastava. 2004. Larval susceptibility of Ajuga rernota against anopheline and culicine mosquitoes. Southeast Asian J. Trop. Med. Public Health 35: 608-610
  15. W.H.O. 1975. Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. Mimeographed document WHO/VBC/75.583
  16. W.H.O. 2005. World Malaria Report, http://www.who.int/ malaria
  17. Zaim, M. 2002. Global Insecticide Use for Vector-Borne Disease Control. Document WHO/CDS/WHOPES/ GCDPP/2002.2. World Health Organization, Geneva