The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Rhizoma Rehmanniae induced Apoptosis in Human Cervical Carcinoma HeLa Cells

생지황(生地黃)이 자궁경부암세포(子宮經部癌細胞)(HeLa cell)에 미치는 영향(影響)

  • Kim, Joo-Yeon (Department of Gynecology, College of Oriental Medicine, Wonkwang University) ;
  • Jo, Ok-Hyon (Department of Gynecology, College of Oriental Medicine, Wonkwang University) ;
  • Choe, Chang-Min (Department of Gynecology, College of Oriental Medicine, Wonkwang University) ;
  • Cho, Han-Baek (Department of Gynecology, College of Oriental Medicine, Wonkwang University)
  • 김주연 (원광대학교 한의과대학 부인과학교실) ;
  • 조옥현 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 부인과학교실)
  • Published : 2006.02.28
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Abstract

Purpose : This study is to evaluate the synergistic cytotoxicity of Rhizoma Rehmanniae(RR), in adriamycin-treated HeLa human cervical carcinoma cells. Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% $CO_2$. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : The combination of RR and adriamycin synergistically augmented the cytotoxicity of HeLa cells. The apoptotic cell death was accompanied by the activation of caspase-3 and -8 as well as cleavage of poly(ADP- ribose) polymerase (PARP) in HeLa cells. The co-treatment of RR with adriamycin didn't have any effect on either the expression of Bcl-2 or that of Bax. Interestingly, a synergistic increase in apoptosis by the combination of two drugs was accompanied by the enhancement of Pas and Fas ligand (FasL) expression in HeLa cells. Taken together, the combination of RR and adriamycin significantly augmented the apoptotic cytotoxicity of Fas-positive cells, such as HeLa cells. The pathway is not involved in mitochondria-dependent pathway. Conclusion : RR induces apoptosis in HeLa cells via p38 MAPK activation.

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