The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Dohaekseungkitang extract induced apoptosis in Human Cervical carcinoma HeLa cells

도핵승기탕(桃核承氣湯) 자궁경부암세포(子宮經部癌細胞)(HeLa cell)의 apoptosis에 미치는 영향(影響)

  • Kang, Yong-Goo (Dept. of genecology, College of Oriental Medicine, Won-Kwang University) ;
  • Ahn, Kyu-Hwan (Dept. of genecology, College of Oriental Medicine, Won-Kwang University) ;
  • Kong, Bok-Cheul (Dept. of genecology, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Song-Baeg (Dept. of genecology, College of Oriental Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of genecology, College of Oriental Medicine, Won-Kwang University)
  • 강용구 (圓光大學校 韓醫科大學 婦人科敎室) ;
  • 안규환 (圓光大學校 韓醫科大學 婦人科敎室) ;
  • 공복철 (圓光大學校 韓醫科大學 婦人科敎室) ;
  • 김송백 (圓光大學校 韓醫科大學 婦人科敎室) ;
  • 조한백 (圓光大學校 韓醫科大學 婦人科敎室)
  • Published : 2006.05.31
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Abstract

Purpose : To address the ability of Dohaekseungkitang (DST: a commonly used herb formulation in Korea, Japan and China to have anti-cancer effect on cervical carcinoma), we investigated the effects of DST on programmed cell death (apoptosis) in HeLa human cervical carcinoma cells. Methods : We cultured HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : After the treatment of DST for 48 hours, apoptosis occurred in a dose-dependent manner. In this study, we have shown that DST induces calpain and the associated caspase-8 and -9 activations. Apoptosis was prevented by pre-incubation of the cells with the calcium cHeLator-BAPTA-AM, calcium channel blocker-Nif edipine or Ryonidine agonist-Ryonidine peptide, implicating calcium in the apoptotic process. Ubiquitous calpains (mu- and m-calpain) have been repeatedly implicated in apoptosis, especially in calcium-related apoptosis. However this study showed 1hat either calpain inhibitor-calpastin or caspase-3 inhibitor-DEVD- did not blocked the herb formulation-induced apoptosis in HeLa human cervical carcinoma cells. D ST initiates a cell death pathway that is partially dependent of caspases. DST-induced apoptosis requires caspase-independent mechanism. Conclusion : We conclude that DST-induced calpain activation triggers the intrinsic apoptotic pathway in which caspase-independent mechanism is also involved.

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