The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effect of Corydalis Tuber on the inhibition of proliferation of human uterine leiomyoma cell and apoptotic gene expression

현호색(玄胡索)이 자궁근종세포의 증식 억제와 Apoptosis 관련 유전자 발현에 미치는 영향

  • Lee, Hee-Jae (Dep. of ynecology, college of Oriental Medicine, Daeguhaany University) ;
  • Baek, Seung-Hee (Dep. of ynecology, college of Oriental Medicine, Daeguhaany University) ;
  • Kim, Dong-Chul (Dep. of ynecology, college of Oriental Medicine, Daeguhaany University)
  • 이희재 (대구한의대학교 한의학과 부인과교실) ;
  • 백승희 (대구한의대학교 한의학과 부인과교실) ;
  • 김동철 (대구한의대학교 한의학과 부인과교실)
  • Published : 2006.05.31
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Abstract

Purpose : This study was aimed to investigate the inhibitory effect of Corydalis Tuber on the proliferation of human uterine leiomyoma cell and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of suvival cells treated with indicated concentration of Corydalis Tuber and investigated cell viability by MTS assay. Furthermore, flow cytometric analyis were used to dissect between necrosis and apoptosis related with cell cycle and then we observed the differential gene expression by western blot analysis. Results : 1) The inhibitory effect on the proliferation of uterine leiomyoma cell treated with Corydalis Tuber was increased in a concentration and time proportional. 2) The result of flow cytometry analysis, subG1 phase arrest related cell apoptosis was not investigated in uterine leiomyoma cell treated Corydalis Tuber but showed G2/M phase prolongation. 3) The gene expression of p27, p21 related cell cycle was increased according to increasing concentration, but p53 was not exchanged. 4) The dephosphorylation of pRb gene were increased dependent on treatment concentration and pro-caspase 3, CDK4 were not exchanged. Conclusion : This study showed that Corydalis Tuber have the inhibitory effect on the proliferation of human uterine leiomyoma cell but the effect was thoughted no relationship with apoptosis. The inhibitory effect was suggested that dephosphorylation of pRb gene induced with increasing p21, p27 prolonged cell division in G2/M phase.

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