Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Coaggregation between Porphyromonas gingivalis and Tannerella forsythia

Porphyromonas gingivalis와 Tannerella forsythia의 응집반응

  • Um, Heung-Sik (Department of Periodontology, College of Dentistry, Kangnung National University) ;
  • Lee, Seok-Woo (Division of Periodontics, School of Dental & Oral Surgery, Columbia University) ;
  • Park, Jae-Hong (Department of Pediatric Dentistry, School of Dentistry, Kyung-Hee University) ;
  • Nauman, R.K. (Department of Microbiology, Baltimore College of Dental Surgery, University of Maryland)
  • Published : 2006.03.30
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Abstract

Dental plaque, a biofilm consisting of more than 500 different bacterial species, is an etiological agent of human periodontal disease, It is therefore important to characterize interactions among periodontopathic microorganisms in order to understand the microbial pathogenesis of periodontal disease. Previous data have suggested a synergistic effect of tow major periodontal pathogens Porphyromonas gingivalis and Tannerella forsythia in the periodontal lesion. In the present study, to better understand interaction between P. gingivalis and T. forsythia, the coaggregation activity between these bacteria was characterized. The coaggregation activity was observed by a direct visual assay by mixing equal amount (1 ${\times}$ $10^9$)of T. forsythia and P. gingivaJis cells. It was found that the first aggregates began to appear after 5-10 min, and that the large aggregates completely settled within 1 h. Electron and epifluorescence microscopic studies confirmed cell-cell contact between two bacteria. The heat treatment of P. gingivalis completely blocked the activity, suggesting an involvement of a heat-labile component of P. gingivalis in the interaction. On the other hand, heat treatment of T. forsythia significantly increased the coaggregation activity; the aggregates began to appear immediately. The coaggregation activity was inhibited by addition of protease, however carbohydrates did not inhibit the activity, suggesting that coaggregation is a protein-protein interaction. The results of this study suggest that coaggregation between P. gingivalis and T. forsythia is a result of cell-cell physical contact, and that coaggregation is mediated by a heat-labile component of P. gingivalis and T. forsythia component that can be activated on heat treatment.

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References

  1. Kolenbrander PE. Coaggregation among oral bacteria. Methods Enzymol 1995; 253: 385-397 https://doi.org/10.1016/S0076-6879(95)53033-9
  2. Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS, and Palmer Jr. RJ. Communication among oral bacteria Microbiol Mol Rev 2002; 66: 486-505 https://doi.org/10.1128/MMBR.66.3.486-505.2002
  3. Kamma JJ, Nakou M, and Manti FA. Periodontal microflora of severe. moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis. J Periodontal Res 1995; 30: 66-72 https://doi.org/10.1111/j.1600-0765.1995.tb01254.x
  4. Yoneda M, Hirofuji T, Anan H, Matsumoto A, Hamachi T, Nakayama K, and Maeda K. Mixed infection of Porphyromonas gingivalis and Bacteroides forsythus in a murine abscess model: Involvement of gingipains in a synergistic effect. J Periodontal Res 2001; 36: 237-243 https://doi.org/10.1034/j.1600-0765.2001.036004237.x
  5. Gmur R, Strub JR, and Guggenheim B. Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. J Perodontal Res 1989; 24: 113-120 https://doi.org/10.1111/j.1600-0765.1989.tb00865.x
  6. Socransky SS, Haffajee AD, Cugini MA, Smith C, and Kent Jr. RL. Microbial complexes in subgingival plaque. J Clin Periodontol 1998; 25:134-144 https://doi.org/10.1111/j.1600-051X.1998.tb02419.x
  7. Fujise O, Hamachi T, Inoue K, Miura M, and Maeda K. Microbiological markers for prediction and assessment of treatment outcome following non-surgical periodontal therapy. J Periodontol 2002; 73: 1253-1259 https://doi.org/10.1902/jop.2002.73.11.1253
  8. Takemoto T, Kurihara H, Dahlen G. Characterization of Bacteroides forsythus isolates. J Clin Microbiol 1997; 35: 1378-1381
  9. Kamaguchi A, Ohyama T, Sakai E, Nakamura R, Watanabe T, Bara H, and Nakayama K. Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for co-aggregation with Prevotella intermedia. Micriobiology 2003; 149: 1257-1264
  10. Fujise O, Miura M, Hamachi T, and Maeda K. Involvement of Prophyromonas goingivalis fimA genotype in treatment outcome following non-surgical periodontal therapy. J Periodontol 2005; 76: 1661-1666 https://doi.org/10.1902/jop.2005.76.10.1661
  11. Sabet M, Lee S-W, Nauman RK, Sims T, and Um H-S. The surface (S-) layer is a virulence factor of Bacteroides forsythus. Microbiol 2003; 149: 3617-3627 https://doi.org/10.1099/mic.0.26535-0