Effect of Mulberry Extract on the Growth of Yogurt Starter Cultures

오디 추출물 첨가가 요구르트 스타터 생육에 미치는 영향

  • Suh Hyung-Joo (Department of Food and Nutrition, Korea University) ;
  • Kim Young-Soon (Department of Food and Nutrition, Korea University) ;
  • Kim Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Lee Ho (Department of Food and Biotechnology, Kyonggi University)
  • 서형주 (고려대학교 식품영양학과) ;
  • 김영순 (고려대학교 식품영양학과) ;
  • 김진만 (건국대학교 축산식품생물공학과) ;
  • 이호 (경기대학교 식품생물공학과)
  • Published : 2006.03.01

Abstract

This experiment was carried out to investigate the effect of mulberry fruit extinct on the acid production and growth of lactic acid bacteria. The extract of mulberry fruit was showed a high level of yield with 60% ethanol extraction. Lactic acid bacteria was used in a starter culture of Lactobacillus casei YIT9018 and 2782, and Lactobacillus acidophilus NCFM. After 24 hr culture in MRS broth added with 1.0, 5.0 and 10% of the extract 1.0% addition of the extract was showed pH $4.04{\sim}4.19$, titratable acidity $1.25{\sim}1.42%,\;and\;1.2{\sim}7.8{\times}10^9\;cfu/mL$ of viable cell count. The additions above 1.0% extract (5.0% and 10.0% addition) showed slightly lower effect than 1.0% addition. However, the addition of the extract showed a high effect on the growth of lactic acid bacteria comparing with the control. In yoghurt preparation with the extract, 1% addition of the extract showed a high effect on the growth of lactic acid bacteria, Therefore, it was suggested to manufacture of the yoghurt with the addition of 1% mulberry fruit extract and the inoculation of culture of Lactobacillus acidophilus for on the stimulation of growth of the lactic culture.

발효 유제품 제조에 오디 추출물을 첨가할 때 유산균 생육에 미치는 오디 추출물의 효과를 측정하여 오디의 이용성을 넓히고자 한다. 오디 추출물은 에탄올 함량이 60%일 때 가장 높은 추출 수율을 보였다. L. casei와 L. acidophilus의 생육에 대한 영향을 측정하기 위해 오디 추출물을 1%, 5%, 10% 첨가시킨 후 MRS 배지에 유산균을 접종하여 24시간 배양후 pH, 총산도 및 생균수를 측정한 결과, 첨가량이 1%일 때 가장 낮은 pH 4.19와 4.11를 보였으며, 생균수 측정시 오디 추출물 무첨가시 $2.3{\times}10^9{\sim}4.2{\times}10^{10}\;CFU/mL$의 생균수를 보인 반면 오디 추출물 첨가시 $1.8{\times}10^9{\sim}7.8{\times}10^{10}\;CFU/mL$의 생균수를 보임에 따라 다소 높은 생균수를 보였다. 또한 총산도 역시 오디 추출물 첨가시 증가하는 경향을 보였다. L. casei와 L. acidophilus을 이용한 발효 유제품 제조시 오디 추출물을 각각 1% 첨가시 발효 유제품에 미치는 영향을 측정한 결과, 오디 추출물을 첨가한 발효 유제품의 산도 및 생균수가 첨가하지 않은 발효 유제품에 비해 높은 경향을 보였다. 오디 추출물 1% 첨가시 산도를 측정한 결과 L. acidophilus을 이용한 발효 유제품의 산도가 가장 낮은 것으로 나타났으며, 생균수 역시 $6.9{\times}10^{10}\;CFU/mL$로 가장 많은 생균수를 보였다.

Keywords

References

  1. Bae, H. C., Cho, I. S., and Nam, M. S. (2004) Fer­mentation properties an dfunctionality of yogurt added with Lycium chinence Miller. J. Anim. Sci. Technol. 46, 687-700 https://doi.org/10.5187/JAST.2004.46.4.687
  2. Fuleki, T. and Francis, F. J. (1968) Quantitative methods for anthocyanins. 1. Extraction and determination of total anthocyanin in cranberries. J. Food Sci. 33, 72-77 https://doi.org/10.1111/j.1365-2621.1968.tb00887.x
  3. Havesteen, B. (1983) Flavonoids, a class of natural products of high pharmacological potency. Biochem. Pharmacol. 32, 1141-1145 https://doi.org/10.1016/0006-2952(83)90262-9
  4. Ishikura, N. A. (1975) Survey of anthocyanins in fruits of some Angiosperms. Int. Bot. Mag. 88, 41-45 https://doi.org/10.1007/BF02498879
  5. Jackman, R. L., Yada, R. Y., and Tung, M. A. (1987) A review: separation and chemical properties of anthocya­nins used for their qualitative and quantitative analysis. J. Food Biochem. 11, 279-308 https://doi.org/10.1111/j.1745-4514.1987.tb00128.x
  6. Kim, H. S. and Ahn, S. Y. (1978) Studies on the formation of anthocyanin metal complex. J. Kor. Agricul. Chem. Soc. 21, 22-30
  7. Kim, S. J., Rhim, J. W., Lee, L. S., and Lee, J. S. (1996) Extraction and characteristics of purple sweet potato pigment. Korean J. Food Sci. Technol. 28, 345-351
  8. Koo, H. H. and Chung, S. H. (1994) Effects of Panax ginseng and Ganoderma lucidum extract on the growth of lactic acid bacteria. J. Korean Soc. Food Sci. Nutr. 7, 45-50
  9. Lee, J. H., Woo, K. J., Choi, W. S., Kim, A. J., and Kim, M. W. (2005) Quality charcateristics of starch Oddi Dasik added with mulberry fruit juice. Korean J. Food Cookery Sci. 21, 629-636
  10. Madigan, M. T., Martinko, J. M., and Parker, J. (2003) Brock biology of microorganisms. 10th ed, Prentice Hall, NY, pp. 504-506
  11. Park, S. O., Yun, O. H., and Kim, H. O. (1980) Studies on the effects of several spices on the growth of Lacto­bacillus casei YIT9018. Korean J. Anim. Sci. 22, 301-309
  12. Timberlake, C. F. (1981) Anthocyanins in fruits and vegetables. In 'Recent Advances in The Biochemistry of Fruits and Vegetables' Friend, J. and Rhodes, M.J.C. (eds.). Academic Press. London. pp. 221-247
  13. Yoo, J. Y., Min, B. Y., Suh, K. B. and Hah, D. M. (1978) Effects of spices on the growth of lactic acid bacteria. Korean J. Food Sci. Technol., 10, 124-135
  14. Yun, D. H. (1980) Effect of cholorella cellular powder on the growth of lactic acid bacteria. M.S. Thesis, Korea Univ., Seoul, Korea