Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced Production of Epothilones by Carbon Sources in Sorangium cellulosum

  • Park Sang-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Choi Sue-Hyung (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Yoon Yeo-Joon (Division of Nano Science, Ewha Women's University) ;
  • Lee Dong-Hyun (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Kim Duk-Joon (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Kim Ji-Heung (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Lee Young-Kwan (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Choi Guang-Jin (Department of Pharmaceutical Engineering, Inje University) ;
  • Yeom Ick-Tae (Department of Civil and Environmental Engineering, Sungkyunkwan University) ;
  • Sim Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University)
  • Published : 2006.04.01
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Abstract

To improve epothilones production and the ratio of epothilone B/A, carbon sources were investigated in flask culture of Sorangium cellulosum. Depending on the initial concentration, starch significantly enhanced cell growth, but the maximum epothilones productivity and the maximum epothilones production (0.3 mg/l day and 2.6 mg/l at 15 g/l starch, respectively) were relatively low compared with cell growth. On the other hand, addition of glycerol did not stimulate cell growth, but epothilone production was increased from 2.81 mg/l to 7.59 mg/l. Addition of glycerol to culture medium resulted in more significant enhancement of the production of epothilone A, whereas epothilone B levels were relatively constant. Furthermore, when sodium propionate was added as a precursor of methylmalonyl-CoA, it resulted in increase of both total epothilones production and epothilone B/A resolution. Maximum epothilone A and B concentrations reached 10.9 mg/l and 8.58 mg/l, respectively, at 5 mM sodium propionate.

Keywords

References

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