Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Promotion of Bone Nodule Formation and Inhibition of Growth and Invasion of Streptococcus mutans by Weissella kimchii PL9001

  • Lee Yeon-Hee (Culture Collection of Antimicrobial Resistant Microbes and Department of Biology, Seoul Women's University)
  • Published : 2006.04.01
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Abstract

Lactic acid-producing bacteria (LABs) are known to have various beneficial properties for health. However, they are generally considered to have an adverse effect on teeth, since they produce acid. Nonetheless, milk and cheese containing specific LAB strains were recently found to have an inhibitory effect on dental caries in children, with an inhibitory activity towards the growth of Streptococcus mutans suggested as the responsible mechanism. Accordingly, the current study selected a probiotic candidate for oral health and studied its inhibitory mechanism against dental caries. Twenty-two LAB species belonging to eleven genuses were screened for promoting bone nodule formation using direct microscopic examination. Only one isolate, Weissella kimchii strain PL9001, increased the bone nodule formation significantly. The addition of W. kimchii strain PL9001 to bone cells prepared from mouse calvaria increased the bone nodule formation, calcium accumulation, and activity of alkaline phosphatase (the osteoblastic marker). Moreover, W. kimchii strain PL9001 inhibited the invasion of Streptococcus mutans into bone cells, and an organic extract of the culture supernatant of W. kimchii strain PL9001 inhibited the growth of Strep. mutans. Therefore, the results suggest that W. kimchii strain PL9001 can be used as a preventive measure against dental caries. This is the first time that a LAB has been shown to promote bone nodule formation and prevent the invasion of Strep. mutans into bone cells.

Keywords

References

  1. Ahola, A. J., H. Yli-Knuuttila, T. Suomalainen, T. Poussa, A. Ahlstrom, J. H. Meurman, and R. Korpela. 2002. Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch. Oral Biol. 47: 799-804 https://doi.org/10.1016/S0003-9969(02)00112-7
  2. Asikainen, S. and S. Alaluusua. 1993. Bacteriology of dental infections. Eur. Heart J. 14(Suppl K): 43-50
  3. Becker, M. R., B. J. Paster, E. J. Leys, M. L. Moeschberger, S. G. Kenyon, J. L. Galvin, S. K. Boches, F. E. Dewhirst, and A. L. Griffen. 2002. Molecular analysis of bacterial species associated with childhood caries. J. Clin. Microbiol. 40: 1001-1009 https://doi.org/10.1128/JCM.40.3.1001-1009.2002
  4. Bellows, C. G., J. E. Aubin, J. N. M. Heersche, and M. E. Antosz. 1986. Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif. Tissue Int. 38: 143-154 https://doi.org/10.1007/BF02556874
  5. Choi, B.-K., H. J. Lee, J. H. Kang, G. J. Jeong, C. K. Min, and Y.-J. Yoo. 2003. Induction of osteoclastogenesis and matrix metalloproteinase expression by the lipooligosaccharide of Treponema denticola. Infect. Immun. 71: 226-233 https://doi.org/10.1128/IAI.71.1.226-233.2003
  6. Choi, H. J., C. I. Cheigh, S. B. Kim, J. C. Lee, D. W. Lee, S. W. Choi, J. M. Park, and Y. R. Byun. 2002. Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. Int. J. Syst. Evol. Microbiol. 52: 507-511 https://doi.org/10.1099/00207713-52-2-507
  7. Chung, J., E. S. Ha, H. R. Park, and S. Kim. 2004. Isolation and characterization of Lactobacillus species inhibiting the formation of Streptococcus mutans biofilm. Oral Microbiol. Immunol. 19: 214-216 https://doi.org/10.1111/j.0902-0055.2004.00137.x
  8. Comelli, E. M., B. Guggenheim, F. Stingele, and J. R. Neeser. 2002. Selection of dairy bacterial strains as probiotics for oral health. Eur. J. Oral Sci. 110: 218-224 https://doi.org/10.1034/j.1600-0447.2002.21216.x
  9. Concannon, S. P., T. D. Crowe, J. J. Abercrombie, C. M. Molina, P. Hou, D. K. Sukumaran, P. A. Raj, and K. P. Leung. 2003. Susceptibility of oral bacteria to an antimicrobial decapeptide. J. Med. Microbiol. 52: 1083-1093 https://doi.org/10.1099/jmm.0.05286-0
  10. Cross, M. L. 2004. Immune-signalling by orally-delivered probiotic bacteria: Effects on common mucosal immunoresponses and protection at distal mucosal sites. Int. J. Immunopathol. Pharmacol. 17: 127-134 https://doi.org/10.1177/039463200401700204
  11. Granath, L., P. Cleaton-Jones, L. P. Fatti, and E. S. Grossman. 1993. Prevalence of dental caries in 4- to 5-year-old children partly explained by presence of salivary mutans streptococci. J. Clin. Microbiol. 31: 66-70
  12. Hagiwara, H., Y. Hiruma, A. Inoue, A. Yamaguchi, and S. Hirose. 1998. Deceleration by angiotensin II of the differentiation and bone formation of rat calvarial osteoblastic cells. J. Endocrinol. 156: 543-550 https://doi.org/10.1677/joe.0.1560543
  13. Harris, R., A. D. Nicoll, P. M. Adair, and C. M. Pine. 2004. Risk factors for dental caries in young children: A systematic review of the literature. Community Dent. Health 21: 71-85
  14. Isolauri, E. 2004. Dietary modification of atopic disease: Use of probiotics in the prevention of atopic dermatitis. Curr. Allergy Asthma Rep. 4: 270-275 https://doi.org/10.1007/s11882-004-0070-9
  15. Jang, J., B. Kim, J. Lee, J. Kim, and G. Jeong, and H. Han. 2002. Identification of Weissella species by the genusspecific amplified ribosomal DNA restriction analysis. FEMS Microbiol. Lett. 212: 29-34 https://doi.org/10.1111/j.1574-6968.2002.tb11240.x
  16. Jevon, M., C. Guo, B. Ma, N. Mordan, S. P. Nair, M. Harris, B. Henderson, G. Bentley, and S. Meghji. 1999. Mechanisms of internalization of Staphylococcus aureus by cultured human osteoblasts. Infect. Immun. 67: 2677-2681
  17. Jiang, Y., C. K. Mehta, T.-Y. Hsu, and F. F. H. Alsulaimani. 2002. Bacteria induce osteoclastogenesis via an osteoblastindependent pathway. Infect. Immun. 70: 3143-3148 https://doi.org/10.1128/IAI.70.6.3143-3148.2002
  18. Kadono, H., J.-I. Kido, M. Kataoka, N. Yamauchi, and T. Nagata. 1999. Inhibition of osteoblastic cell differentiation by lipopolysaccharide extract from Porphyromonas gingivalis. Infect. Immun. 67: 2841-2846
  19. Kakuta, H., Y. Iwami, H. Mayanagi, and N. Takahashi. 2003. Xylitol inhibition of acid production and growth of mutans Streptococci in the presence of various dietary sugars under strictly anaerobic conditions. Caries Res. 37: 404-409 https://doi.org/10.1159/000073391
  20. Kolho, K.-L., P. Holtta, S. Alaluusua, H. Lindahl, E. Savilahti, and H. Rautelin. 2001. Dental caries is common in Finnish children infected with Helicobacter pylori. Scan. J. Infect. Dis. 33: 815-817 https://doi.org/10.1080/00365540110076624
  21. Lee, H. Y., J. H. Park, S. H. Seok, S. A. Cho, M. W. Baek, D. J. Kim, Y. Lee, and J. H. Park. J. 2004. Dietary intake of various lactic acid bacteria suppresses type 2 helper T cell production in antigen-primed mice splenocyte. J. Microbiol. Biotechnol. 14: 167-170 https://doi.org/10.1159/000078108
  22. Lee, Y. 2005. Characterization of Weissella kimchii PL9023 as a potential probiotic for women. FEMS Microbiol. Lett. 250: 157-162 https://doi.org/10.1016/j.femsle.2005.07.009
  23. Menard, S., C. Candalh, J. C. Bambou, K. Terpend, N. Cerf- Bensussan, and M. Heyman. 2004. Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport. Gut 53: 821-828 https://doi.org/10.1136/gut.2003.026252
  24. Mitchell, T. J. 2003. The pathogenesis of streptococcal infections: From tooth decay to meningitis. Nat. Rev. Microbiol. 1: 219-230 https://doi.org/10.1038/nrmicro771
  25. Mitzi, R. B., B. J. Paster, E. J. Leys, M. L. Moeschberger, S. G. Kenyon, J. L. Galvin, S. K. Boches, F. E. Dewhirst, and A. L. Griffen. 2002. Molecular analysis of bacterial species associated with childhood caries. J. Clin. Microbiol. 40: 1001-1009 https://doi.org/10.1128/JCM.40.3.1001-1009.2002
  26. Montalto, M., M. Vastola, L. Marigo, M. Covino, R. Graziosetto, V. Curigliano, L. Santoro, L. Cuoco, R. Manna, and G. Gasbarrini. 2004. Probiotic treatment increases salivary counts of lactobacilli: A double-blind, randomized, controlled study. Digestion 69: 53-56 https://doi.org/10.1159/000076559
  27. Nam, H., M. Ha, O. Bae, and Y. Lee. 2002. Effect of Weissella confusa strain PL9001 on the adherence and growth of Helicobacter pylori. Appl. Environ. Microbiol. 68: 4642-4645 https://doi.org/10.1128/AEM.68.9.4642-4645.2002
  28. Nase, L., K. Hatakka, E. Savilahti, M. Saxelin, A. Ponka, T. Poussa, R. Korpela, and J. H. Meurman. 2001. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 35: 412-420 https://doi.org/10.1159/000047484
  29. Ocana, V. S. and M. E. Nader-Macias. 2004. Production of antimicrobial substances by lactic acid bacteria II: Screening bacteriocin-producing strains with probiotic purposes and characterization of a Lactobacillus bacteriocin. Methods Mol. Biol. 268: 347-354
  30. Oelschlaeger, T. A. and B. A. Tall. 1996. Uptake pathways of clinical isolates of Proteus mirabilis into human epithelial cell lines. Microb. Pathog. 21: 1-16 https://doi.org/10.1006/mpat.1996.0037
  31. Reid, G., M. E. Sanders, H. R. Gaskins, G. R. Gibson, A. Mercenier, R. Rastall, M. Roberfroid, I. Rowland, C. Cherbut, and T. R. Klaenhammer. 2003. New scientific paradigms for probiotics and prebiotics. J. Clin. Gastroenterol. 37: 105-118 https://doi.org/10.1097/00004836-200308000-00004
  32. Sartor, R. B. 2004. Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: Antibiotics, probiotics, and prebiotics. Gastroenterology 126: 1620- 1633 https://doi.org/10.1053/j.gastro.2004.03.024
  33. Shimazaki, Y., M. Mitoma, T. Oho, Y. Nakano, Y. Yamashita, K. Okano, Y. Nakano, M. Fukuyama, N. Fujihara, Y. Nada, and T. Koga. 2001. Passive immunization with milk produced from an immunized cow prevents oral recolonization by Streptococcus mutans. Clin. Diagn. Lab. Immunol. 86: 1136-1139
  34. Takarada, K., R. Kimizuka, N. Takahashi, K. Honma, K. Okuda, and T. Kato. 2004. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol. Immunol. 19: 61-64 https://doi.org/10.1046/j.0902-0055.2003.00111.x
  35. Tomas, M. S., O. M. Claudia, V. Ocana, and M. E. Nader- Macias. 2004. Production of antimicrobial substances by lactic acid bacteria I: Determination of hydrogen peroxide. Methods Mol. Biol. 268: 337-346
  36. Turchet, P., M. Laurenzano, S. Auboiron, and J. M. Antoine. 2003. Effect of fermented milk containing the probiotic Lactobacillus casei DN-114001 on winter infections in freeliving elderly subjects: A randomized, controlled pilot study. J. Nutr. Health Aging 7: 5-77
  37. Wei, H., V. Loimaranta, J. Tenovuo, S. Rokka, E. L. Syvaoja, H. Korhonen, V. Joutsjoki, and P. Marnila. 2002. Stability and activity of specific antibodies against Streptococcus mutans and Streptococcus sobrinus in bovine milk fermented with Lactobacillus rhamnosus strain GG or treated at ultra-high temperature. Oral Microbiol. Immunol. 17: 9-15 https://doi.org/10.1046/j.0902-0055.2001.00084.x