Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immune-Enhancing Alkali-Soluble Glucans Produced by Wild-Type and Mutant Saccharomyces cerevisiae

  • Ha Chang-Hoon (School of Life Science and Biotechnology, Korea University) ;
  • Lim Ki-Hong (School of Life Science and Biotechnology, Korea University) ;
  • Jang Se-Hwan (School of Life Science and Biotechnology, Korea University) ;
  • Yun Cheol-Won (School of Life Science and Biotechnology, Korea University) ;
  • Paik Hyun-Dong (Division of Animal Life Science, Kon-Kuk University) ;
  • Kim Seung-Wook (Department of Chemical and Biological Engineering, Korea University) ;
  • Kang Chang-Won (Division of Animal Life Science, Kon-Kuk University) ;
  • Chang Hyo-Ihl (School of Life Science and Biotechnology, Korea University)
  • Published : 2006.04.01
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Abstract

The alkali-soluble glucan of the yeast cell wall contains $\beta-(1,3)-$ and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase $(endo-\beta-(1,3)-D-glucanase)$. The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of $\beta-(1,6)-D-linkage$ than was observed in conjunction with the wild-type. Yeast cell wall $\beta-glucan$ was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha $(TNF-\alpha)$ and nitric oxide. Alkali-soluble $\beta-glucans$, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha $(TNF-\alpha)$ and nitric oxide and direct phagocytosis, than did the positive control ($1{\mu}g$ of lipopolysaccharide).

Keywords

References

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