Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Differentiation of Lymphocystis Disease Virus Genotype by Multiplex PCR

  • Kitamura Shin Ichi (Department of Aqualife Medicine, Chonnam National University) ;
  • Jung Sung-Ju (Department of Aqualife Medicine, Chonnam National University) ;
  • Oh Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • Published : 2006.04.01
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Abstract

Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. The viruses have been divided into three genotypes (genotype I for LCDV-1, II for Japanese flounder isolates, and III for rockfish isolates) on the basis of major capsid protein (MCP) gene sequences. In this study, we developed a multiplex PCR primer set in order to distinguish these genotypes. We also analyzed the MCP gene of a new LCDV isolate from the sea bass (SB98Yosu). Comparison of sequence identities between SB98Yosu and eight Japanese flounder isolates, revealed identity of more than 90.1 % at nucleotide level and 96.5% at deduced amino acid level, respectively. Phylogenetic analyses based on the MCP gene showed that SB98Yosu belongs to genotype II, along with Japanese flounder isolates. Multiplex PCR based on the MCP gene allowed us to identify these genotypes in a simple and rapid manner, even in a sample that contained two genotypes, in this case genotypes II and III.

Keywords

References

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