Optimal Culture Conditions for Production of Yellow Pigments from Monascus purpureus in Liquid Culture

액체 배양에 의한 Monascus purpureus가 생산하는 황색색소의 최적 생산조건

  • Choi Chung-Sig (HansBio Co., BI center 303, Andong National University) ;
  • Jeon Chun-Pyo (School of Bioresource Sciences, Andong National University) ;
  • Lee Jung-Bok (School of Bioresource Sciences, Andong National University) ;
  • Lee Oh-Seuk (Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • Rhee Chang-Ho (Gyeongbuk Institute for Bio Industry) ;
  • Kwon Gi-Seok (School of Bioresource Sciences, Andong National University)
  • Published : 2006.04.01

Abstract

The optimum cultural conditions for production of yellow pigment by Monascus purpureus MMK2 were investigated in liquid culture. Monascus purpureus MMK2 was shown to give the maximum production of yellow pigment in the medium containing of 3.0% wheat flour, 0.15% $NaNO_3$, 0.25% $Na_2HPO_4\;12H_2O\;and\;0.15%\;MgSO_4\;7H_2O$. The optimal culture conditions for temperature and initial pH were $30^{\circ}C$ and 6.5, respectively. The yellow pigment production reached a maximum level at the 7th day of cultivation.

Monascus purpureus MMK2를 이용하여 황색색소 생산을 위한 배양 조건의 최적화에 관한 결과는 다음과 같다. 실험 균주가 생산하는 황색색소의 최적 배양 조건은 탄소원으로 wheat flour 3.0% 첨가, 질소원으로 $NaNO_3$ 0.15%, 인산염으로 $Na_2HPO_4\;12H_2O$의 농도가 0.25% 및 $MgSO_4\;7H_2O$의 농도가 0.15%일 때 가장 높은 황색색소 생성을 나타내었다. 배양 온도는 $30^{\circ}C$, 초기 PH가 6.5 및 배양 시간 7일 일 때 황색색소의 생성능이 28.91 unit로 가장 우수하였다.

Keywords

References

  1. Judie, D.D. (1987) Applications and colorants. Food Technol., 23, 78-88
  2. Kim, J.Y. and Kim, K.H. (1997) Isolation and characterization of Bacillus sp. PY123 producing water-soluble yellow pigment. Kor. J. Appl. Microbiol. Biotechnol., 25, 454-458
  3. Lin, C.F. and Iizuka, H. (1982) Production of Extracellular pigments by mutant of Monascus kaoliang sp. nov. Appl. Environ. Microbiol., 43, 671-676
  4. Kang, S.G., Rhim, J.W., Jung, S.T. and Kim, S.J. (1996) Production of Red and Yellow Pigments from Monascus anka in a Jar Fermenter. Kor. J. Appl. Microbiol. Biotechnol., 24, 756-762
  5. Kim, C.S., Rhee, S.H. and Kim, I. (1977) Studies on production and characteristics of edible red color pigment produced by mold (Monascus sp.). Kor. J. Food Sci. Technol., 9, 277-283
  6. Kumasaki, S., Nakanishi, K., Nishikawa, E. and Ohashi, M. (1962) Structure of monascorubrin. Tetrahedron., 18, 1171 https://doi.org/10.1016/S0040-4020(01)99285-3
  7. Lin, T.F. (1983) The identifivation and practical classification of Monascus sp. (In Chinese), Spec. Top. Sci. Technol. Alc. Bevs., 5, 104-113
  8. Manchand, P.S. and Whalley, W.B. (1989) Isolation and structure of ankaflavin : A new pigment from Monascus anka. Phytochemistry., 12, 2531-2532
  9. Huang, T.L. (1981) Fermentative production and toxic test of natural pigment-Monascus pigments. M.S.T., National Taiwan University. Taipei, Taiwan
  10. Su, Y.C. and Wang, W.H. (1983) Chinese red rice : Anka. In: Handbook of Indigenous Fermented Foods. Marcel Dekker. p.547-553
  11. Lee, Y.K., Chen, D.C., Chauvatcharin, S., Seki, T. and Yoshida, T. (1995) Production of Monascus pigments by a soild-liquid state culture method. J. Ferment. Bio., 79, 516-518 https://doi.org/10.1016/0922-338X(95)91275-A
  12. Nishikawa, E. (1932) Studies on the biochemistry of mold. The pigments of Monascus purpureus Went. J. Agric. Chem. Soc. Jpn., 8, 1007-1011
  13. Kim, S.Y. and Kim, J.K. (1990) Pigment production in Monascus anka, J. Korean Agric. Chem. Soc., 3, 239-246
  14. Kim, H.S., Kim, D.H., Yang, H.S., Pyen, Y.R. and Yu, J.H. (1979) Studies on the yellow pigment produced by Monascus sp. in submerged culture. Patr I. Isolation of strain and cultural conditions of pigment produced. Kor. J. Appl. Microbiol. Bioeng., 7, 23-30
  15. Carels, M. and Shepherd, D. (1977) The effect of different nitrogen sources on pigment production and sporulation of Monascus sp. it submerged shaken culture. Can. J. Microbiol., 23, 1360-1365 https://doi.org/10.1139/m77-205
  16. Lin, C.F. (1973) Isolation and cultural conditions of Monascus sp. for the production of pigment in a submerged culture. J. Ferment Technol., 51, 107-114
  17. Evans, P.J. and Wong, H.Y. (1984) Pigment production from immobilized Monascus sp. utilizing polymeric resin adsorption. Appl. Environ. Microbiol., 47, 1323-1326
  18. Wong, H.C. and Bau, T.S. (1977) Pigmentation and antibacterial activity of fast neutron- and X-ray-induced strains of Monascus purpureus Went. Plant. Physiol., 60, 578-581 https://doi.org/10.1104/pp.60.4.578
  19. Park, C.D., Jung, H.J. and Yu, T.S. (2005) Optimization of Pigment production of Monascus purpureus P-57 in Liquid Culture. Korean J. Bio. Bioeng., 20, 66-70
  20. Seo, S.G., Lee, C.H. and Woo, C.J. (2004) Studies on the Optimal Culture Condition for Production of Red Pigments by Monascus rubber on Liquid Culture. Korean J. Food Pre., 11, 111-116
  21. Kim, H.S., Kim, D.H., Yang, H.S., Pyen, Y.R., and Yu, J.H. (1979) Studies on the yellow pigment produced by Monascus sp. in submerged culture. Patr I. Isolation of strain and cultural conditions of pigment produced. Kor. J. Appl. Microbiol. Bioeng., 7, 23-30
  22. Kim, H.G., Park, G.T. and Son, H.J. (1998) Characterization of Red Pigment Production by Monascus anka. Korean J. Food and Nutr., 11, 612-616
  23. Juzlova, P., Martinkova, L. and Kren, V. (1996) Secondary metabolites of the fungus Monascus: a review. J. Ind. Microbiol., 16, 163-170 https://doi.org/10.1007/BF01569999
  24. Yoshimura, M., Yamanaka, S., Mitsugi, K. and Hirose, Y. (1975) Production of Monascus-pigment in suhmerged culture. Agri. Biol. Chem., 39, 1789-1795 https://doi.org/10.1271/bbb1961.39.1789
  25. Su, Y.C. (1983) Fermentative production of anka-pigments (Monascus-pigment). Kor. J. Appl. Microbiol. Bioeng., 11, 325-337
  26. Chang, U., Kim, H.S., Son, C.H., Bae, J.C. and Yu, J.H. (1980) Studies on the yellow pigment produced by Monascus sp. CS-2; Patr I. Cultural conditions for yellow pigment production, Kor. J. Appl. Microbial. Bioeng., 8, 119-123