Physicochemical Analysis and Antioxidative Effects of Wild Grape (Vitis coignetiea) Juice and Its Wine

머루즙과 머루주의 이화학적 분석 및 항산화 효과

  • Choi, Sun-Young (Dept. Food Science and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Cho, Hyun-So (Dept. Food Science and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Kim, Haeng-Ja (Dept. of Home Economics Education The Principal Researcher of Education Research Institute, Gyeongsang National University) ;
  • Ryu, Chung-Ho (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jeong-Ok (Division of Applied Life Science, Gyeongsang National University) ;
  • Sung, Nak-Ju (Dept. Food Science and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 최선영 (경상대학교 식품영양학과 농업생명과학연구원) ;
  • 조현소 (경상대학교 식품영양학과 농업생명과학연구원) ;
  • 김행자 (경상대학교 가정교육과 교육연구원) ;
  • 류충호 (경상대학교 응용생명과학부) ;
  • 이정옥 (경상대학교 응용생명과학부) ;
  • 성낙주 (경상대학교 식품영양학과 농업생명과학연구원)
  • Published : 2006.09.30

Abstract

Physicochemical analysis of the wild grape juice and its wine extracted with ethanol were assessed. The contents of moisture in wild grape juice and its wine were 78.7${\pm}$0.2% and 74.4${\pm}$O.4%, respectively. Total anthocyanin and flavonoid contents of wild grape juice were higher 4 times and 10 times than those of wild grape wine, respectively. Of the 17 amino acids, the glutamic acid in wild gape juice and threonine in wild grape wine were the highest, which were 43.7${\pm}$1.4mg/100 g and 14.1${\pm}$0.7 mg/100 g, respectively. Electron donating abilities of wild grape juice and its wine at concentration of 1,000 ${\mu}$g/ml were 93.1${\pm}$1.2% and 88.9${\pm}$O.8%, respectively. The nitrite scavenging abilities of wild grape juice and its wine were gradually increased by increasing levels of the extracts to 79.6${\pm}$1.27% and 72.8${\pm}$1.01% at concentration of 1,000 ${\mu}$g/ml under pH 2.5.

머루의 기능성 식품 개발을 위한 기초 자료를 얻고자 머루즙과 머루주를 80% ethanol로 추출하여 이화학적 분석과 항산화 활성을 측정하였다. 머루즙과 머루주의 수분 함량은 70% 이상이었고, 총 안토시아닌은 머루주보다 머루즙에서 약 4배, 플라보노이드는 약 10배 정도로 높게 정량되었다. 구성 아미노산은 총 11종이 분석되었으며, 그 함량은 머루즙에서는 glutamic acid (43.7${\pm}$l.4 mg/100 g) 그리고 머루주에서는 threonine(14.7${\pm}$0.7 mg/100 g)이 가장 높게 정량되었다. 정량된 10종의 무기물 중 K의 함량이 머루즙과 머루주에서 44.1${\pm}$2.0 mg/100 g, 44.8${\pm}$3.1 mg/100 g으로 가장 많았다. 항산화 효과를 실험한 결과, 시료의 농도가 증가함에 따라 전자 공여능과 환원력은 유의적으로 높아지는 경향이었다. 머루즙과 머루주를 농도별로 첨가하여 아질산염 소거 작용을 실험한 결과 낮은 pH에서 농도가 증가할수록 활성이 증가하여 1,000 ${\mu}$g/ml의 처리구의 머루즙과 머루주에서 각각 79.6${\pm}$1.27% 및 72.8${\pm}$1.01%의 소거능을 보였다.

Keywords

References

  1. Park, SY and Kim, JW. Screening and isolation of the antitumor agents from medicinal plants (1). Kor. J. Pharmacogn. 23:264-267. 1992
  2. Lee, JH and Lee, SR Some physiological activity of phenolic substance in plant foods. Korean J. Food Sci. Technol. 26:317-323. 1994
  3. Lee, JH and Lee, SR Analysis of phenolic substances content in Korean plant foods. Korean J. Sci. Thechnol. 26:310-316. 1994
  4. Yang, CS, Landau, JM and Huang, MT. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu. Rev. Nutr. 21:381-406. 2001 https://doi.org/10.1146/annurev.nutr.21.1.381
  5. Hope, WC, Welton, AF, Fielder-Nagy, C, Batula-Bernado, C and Coffey, JC. In vitro inhibition of the biosynthesis of slow reacting substances of anaphylaxis(SRS-A) and lipoxygenase activity of quercetin. Biochem. Pharmacol. 32: 367-371. 1983 https://doi.org/10.1016/0006-2952(83)90569-5
  6. Duarte, J, Vizcaino, FP, Utrilla, P, Tamargo, J and Zarzuelo, A. Vasodilatory effects of flavonoids in rat aortic smooth muscle: structure activity relationships. Biochem. 24:857-862. 1993
  7. Cheon, KB. Screening of antioxidant from Vitis coignenetae, Vitis vinifera L. and comparition of its antioxidant activity. Master Thesis, The Kon Kuk Univ., Korea. pp.12. 1999
  8. Jiangsu New Medical College. Dictionary of Chinese traditional medicine. Shanghai. Vol 2, pp.2315. 1997
  9. Kai, SH, Mao, L, Lin, NY and Man, K. Four novel oilgostilbenes from the root of Vitis amurensis. Tetrahedron. 56:1321-1329. 2000 https://doi.org/10.1016/S0040-4020(99)01034-0
  10. Kim, SY and Kim, SK. Winemaking from new wild grape. Korean J. Food Nutr. 10:254-262. 1997
  11. Kim, SK Deacidification of new wild grape wine. Korean J. Food Nutr. 9:265-270. 1996
  12. Lee, JE, Won, YD, Kim, SS and Koh, KH. The chemical characteristics of Korean red wine with different grape varieties. Korean J. Food Sci. Technol. 34:151-156. 2002
  13. Lee, JE, Shin, YS, Sim, JK, Kim, SS and Kob, KH. Study on the color characteristics of Korean red wine. Korean J. Food Sci. Technol. 34:164-169. 2002
  14. Morris, JR, Sistrunk, WA, Junek, J and Sims, CA. Effect of fruit maturity, juice storage and juice extraction temperature on quality of concord grape juice. J. Amer. Soc. Hort. Sci. 111:742-746. 1986
  15. Gutfinger, T. Polyphenols in olive oil. J. Am. Oil Chem. Soc. 58:966-968. 1958 https://doi.org/10.1007/BF02659771
  16. Moreno, MIN, Isla, MIN, Sampietro, AR and Vattuone, MA. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J. Enthropharmacology 71:109-114. 2000 https://doi.org/10.1016/S0378-8741(99)00189-0
  17. Kang, YH, Park, YK and Lee, GD. The nitrite scavenging and electron donating ability of phenolic compounds. Korean Food Sci. Technol. 28:232-239. 1996
  18. Oyaizu, M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J. Nutr. 44:307-315. 1986 https://doi.org/10.5264/eiyogakuzashi.44.307
  19. Kato, H, Lee, IE, Chyen, NS, Kim, B and Hayase, F. Uninhibitory of nitrosamine formation by nondialyzable melannoins. Agric. Biol. Chem. 51:1333-1338. 1987 https://doi.org/10.1271/bbb1961.51.1333
  20. 박홍주, 백오현, 장학길, 이동태, 이서래. 국내식품 자원의 영양가 분석. 농촌 영양개선 연구조사 사업 보고서. 농영연-연조-10. 1986
  21. Okuda, K and Yokotsuka, K. Trans-resveratrol concentrations in berry skin and wines from grapes grown in Japan. Am. J. Enol. Vitic. 47:93-99. 1996
  22. Ritchey, JG and Waterhouse, AL. Standard red wine: monomeric phenolic analysis of commercial cabernet sauvignon wines. Am. J. Enol. Vitic. 50:91-100. 1999
  23. Shin, JH, Choi, DJ and Sung, NJ. Nutritional properties Yakju brewed with natural plants. Korean J. Food Nutr. 17:18-24. 2004
  24. Whang, HJ and Kim, SS. Analysis of mineral in Korean apple juice by inductively coupled plasma. Korean J. Food Nutr. 12:344-349. 1999
  25. Nam, JH and Joo, KJ. Phenolic compounds and antioxidant capacity of some selected fruit juices and fermented grape juice. J. East Asian Soc. Dietary Life 14:501-507. 2004
  26. Cha, WS, Shin, HR, Park, JH, Oh, SL, Lee, WY, Chun, SS, Choo, JW and Chun, YJ. Antioxidant activity of phenolic compounds from mulberry fruits. Korean J. Food Preservation 11:383-387. 2004
  27. Kim, HK, Na, GM, Ye, SH and Han, HS. Extraction characteristics and antioxidative activity of Schiznadra chinensis extracts. Korean J. Food Culture 19:484-490. 2004
  28. Seo, SB, Han, SM, Kim, JH, Kim, NM and Lee, JS. Manufacture and physiological functionality of wines and wines by using Plum(Prunus salicina). Korean J. Biotechnol. Bioeng. 16:153-157. 2001