Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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The Effect of Korean Red Ginseng Water-extract on Aging-related Changes in the Xenobiotic Metabolizing Enzyme System in the Liver of Rats

홍삼의 물추출물이 노화에 따른 흰쥐 간의 이물질대사 효소계에 미치는 영향

  • Published : 2006.12.30
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Abstract

The present study was designed to investigate aging-related effects on the activities of xenobiotic metabolizing enzymes of rat liver by dietary supplementation of Korean red ginseng water-extract. Rat did not show any discernible signs of the rejection symptoms, and blood GOT and GPT levels were not influenced by ginseng water extracts, Cytochrome 450 levels and NADPH cytochrome P45O reductase, p-450 dependent ethoxycoumarin O-deethylase, and benzphetamine N-demethylase activities were decreased with aging, however, these phase I system enzymes activities in the ginseng group of24 months were well maintained compared with normal group. But, Levels of cytochrome bs and NADH-cytochrome b$_5$ reductase activities were also decreased with aging and were not found a clear difference between two groups. Glutathione-s-transferase activity, phase II enzyme system, in liver cytosols was also decreased in old ages, but the degree of decrease was higher in normal group than in giuseng supplemented group. These results indicate that long-term supplementation of red ginseng water extracts from weaning to 24 months do not show any side effects to rats, and retard age-related deteriorations of xenobiotic metabolizing enzymes activities in old ages.

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References

  1. Attele AS, Zhou YP, Xie JT, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS, Yana CS (2002) Antidiabetic effects of Panax ginseng berry extract and the identification of an effective components. Diabetes. 51: 1851-1858 https://doi.org/10.2337/diabetes.51.6.1851
  2. Bansal SK, Love J, Gurtoo HL (1983) High pressure liquid chromatographic separation of multiple forms of cytochrome P450. Biochem. Biophys. Res. Commun. 117:268-274 https://doi.org/10.1016/0006-291X(83)91570-X
  3. Birnbaum LS (1980) Altered hepatic drug metabolism in senescent mice. Exp. Geronto. 15:259-267 https://doi.org/10.1016/0531-5565(80)90030-3
  4. Blanco P, Machado A, Satrustegui J (1987) Variations due to hypoxia and ageing in the activities of glutathione-S-transferase and NADPH-cytochrome C reductase. Mech. Ageing Dev. 39:11-19 https://doi.org/10.1016/0047-6374(87)90082-0
  5. Choi HK, Seong DH, Rha KH (1995) Clinical efficacy of Korean red ginseng for erectile dysfunction. IntI. J. Impot. Res. 7:181-186
  6. Debersac P, Vernevaut MF, Amiot MJ, Suschetet M, Siess MH (2001) Effects of a water-soluble extract of rosemary and its purified component rosmarinic acid on xenobiotic-metabolizing enzymes in rat liver. Food Chem. Toxicol. 39:109-117 https://doi.org/10.1016/S0278-6915(00)00117-4
  7. Fujita S, Katagawa H, Ishizawa H, Suzuki T, Kitani K (1985) Age-associated alterations in hepatic glutathione-S-transferase activities. Biochem. Pharmacol. 34:3891-3894 https://doi.org/10.1016/0006-2952(85)90440-X
  8. Greenlee WF, Poland A(1978) An improved assay of 7-ethoxycoumarine O-deethylase activity. Induction of hepatic enzyme activity in C57BL/6J and DBA/2J mice by phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachloro-dibenzo-p-doxin. J. Phamacol. Exp. Ther. 251:1385-1391
  9. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-Stransferase. The first enzymatic step in mercapturic acid formation. J. BioI. Chem. 249:7130-7139
  10. Hartley DE, Elsabagh S, File SE (2004) Gincosan(a combination of Ginkco biloba and Panax ginseng) : the effects on mood and cognition of 6 and 12 weeks treatment in post-menopausal women. Nutr. Neurosci. 7:325-333 https://doi.org/10.1080/10284150400015557
  11. Hartz AT, Bertler S, Noyes R, Hoehms J, Logemann C, Schnift S, Butain Y, Wang W, Brake K, Erake M, Kautzman H (2004) Randomized controlled fatigue trial of Siberian ginseng for chronic fatigue. Psychol Med. 34:51-61 https://doi.org/10.1017/S0033291703008791
  12. Jeong CS, Hyun JE, Kim YS (2003) Ginsenoside Rb1: the antiulcer constituents from the head of Panax ginseng. Arch. Pharm. Res. 26:906-911 https://doi.org/10.1007/BF02980198
  13. Kenyon C (2005) The plasticity of aging: insights from long-lived mutants. Cell. 120:449-460 https://doi.org/10.1016/j.cell.2005.02.002
  14. Kimura Y, Okuda H, Arichi S (1988) Effects of various ginseng saponins on 5-hydroxytryptamine release and aggregation in human platelets. J. Pharm. Pharmacol. 40:838-843 https://doi.org/10.1111/j.2042-7158.1988.tb06285.x
  15. Lee SH, Jang BH, Kim SY, Lee EH, Chung BC (2006a) The antistress effect of ginseng total saponin and ginsenoside Rg3 and Rb1 evaluated by brain polyamine level under immobilization stress. Pharmacol Res. 54:46-49 https://doi.org/10.1016/j.phrs.2006.02.001
  16. Lee SE, Seung NS, Bang JK, Kang SW, Lee SW, Chung TY (2003) Inhibibitory effect against angiotensin converting enzyme and antioxidant activity of panax ginseng C.A. meyer extracts. Kor. J. Med. Crop. Sci. 11:236-245
  17. Lee SE, Sung WL, Bang JK, Yu YJ, Seong NS (2004) Antioxidant activities of leaf, stem and root of panax ginseng C. A. meyer. Kor. J. Med. Crop. Sci. 12:237-242
  18. Lee WK, Kae ST, Liu IM, Cheng JT (2006b) : Increase of insulin secretion by ginsenoside Rb2 to lower plasma glucose in wistar rats. Clin. Exp. Pharmacol. Physio. 33:27-32 https://doi.org/10.1111/j.1440-1681.2006.04319.x
  19. Li G, Wang Z, Sun Y, Liu K, Wang Z (2006) Ginsenoside 20(S)-protopanaxadiol inhibits the proliferation and invasion of human fibrosarcoma HT1080 cell. Basic Clin. Pharmacol. Toxicol. 98:588-592 https://doi.org/10.1111/j.1742-7843.2006.pto_415.x
  20. Lim H.B, Sohn HO, Lee YG, Lee DW (1997) Effect of food restriction on age-related changes of liver microsomal cytochrome P450 system. Kor. J. Gerontol. 7:29-33
  21. Lim HB, Sohn HO, Lee DW (2005) Physiological changes with age by the chronic administration of Korean red ginseng in Spraque-Dawley rats. Kor. J. Med. Crop. Sci. 13:242-248
  22. Lin JH, Lu AY (2001) Inhibition and induction of cytochrome P450 and the clinical implications. Clin. Pharmacokinet. 35:361-390 https://doi.org/10.2165/00003088-199835050-00003
  23. Liu LP, Liu JF, Lu YO (2001) Effect of Sherg-Mai injection on the RRPP synthetase activity in BFU-es and CFU-es from bone marrows of mice with benzen-induced aplastic anemia. Life Sci. 69:1373-1379 https://doi.org/10.1016/S0024-3205(01)01224-3
  24. Lowry OH, Rosebrough HJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J. BioI. Chem. 193:265-275
  25. McLean AJ, Le Couteur DG (2004) Aging biology and geriatric clinical pharmacology. Pharmacol. Rev. 56: 163-184 https://doi.org/10.1124/pr.56.2.4
  26. Mihara K, Sato R (1975) Partial purification of NADH-cytochrome b5 reductase from rabbit liver microsomes with detergents and its properties. J. Biochem. Tokyo 71:725-735
  27. Nachtiqual, MC, Patterson RE, Stratton KL, Adams LA, Shattuck AL, White E (2005) Dietary supplements and weight control in a middle-age population. J. Altern. Complement Med. 11:909-915 https://doi.org/10.1089/acm.2005.11.909
  28. Nakagima T, Wang RS, Katakura Y, Kishi R, Elovara E, Park SS, Gelboin HV, Vainio H (1992) Sex-, age- and pregnancyinduced changes in the metabolism of toluene and trichloroethylene in rat liver in relation to the regulation of cytochrome P450IIE1 and P450IIC11 content. J. Pharmacol. Exp. Ther. 261:849-874
  29. Omura T, Sato R (1964) The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. BioI. Chem. 239:2370-2378
  30. Plewka A, Kaminski M, Plewka D (1998) Ontogenesis of hepatocyte respiration processes in relation to rat liver cytochrome P450-dependent monooxygenase system. Mech. Ageing. 105:197-207 https://doi.org/10.1016/S0047-6374(98)00086-4
  31. Rivera E, Ekholm Pettersson F, Inganas M, Paulie S, Gronvik KO (2005) The Rb1 fraction of ginseng elicits a balanced Th1 and Th2 immune response. Vaccine 23:5411-5419 https://doi.org/10.1016/j.vaccine.2005.04.007
  32. Ryff CD, Singer BH (2005) Social environments and genetics of aging : advancing knowledge of protective health mechanisms. J. Gerontol. Psychol. Sci. Soc. Sci. 60:12-23 https://doi.org/10.1093/geronb/60.Special_Issue_1.12
  33. Schenkman JB, Jansson I (1999) Interactions between cytochrome P450 and cytochrome b5. Drug Metab. Rev. 31:351-364 https://doi.org/10.1081/DMR-100101923
  34. Sharma SD, Iqbal M (2005) Lithium induced toxicity in rats : a hematological, biochemical and histopathological study. BioI. Pharm. Bull. 28:834-837 https://doi.org/10.1248/bpb.28.834
  35. Shin HJ, Kim YS, Kwak YS, Song YB, Kim YS, Park JD (2004) Enhancement of antitumor effects of paclitaxel(taxol) in combination with red ginseng acidic polysaccharide. Planta Med. 70:1033-1038 https://doi.org/10.1055/s-2004-832643
  36. Shin JY, Song JY, Yun YS, Yang HO, Rhee DK, Pyo S (2002) Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol. Immunotoxicol. 24:469-482 https://doi.org/10.1081/IPH-120014730
  37. Stohs SJ, AI-Turk WA Angel CR (1982) Glutathione-Stransferase and glutathione reductase activities in hepatic and extra-hepatic tissues of female mice as a function of age. Biochem. Pharmacol. 31:2113-2116 https://doi.org/10.1016/0006-2952(82)90429-4
  38. Thomas PE, Lu AYH, Ryant D, West SB, Kawarek J, Levin W (1976) Multiple forms of rat liver cytochrome P-450. Immunochemical evidence with antibody against cytochrome P-448. J. BioI. Chem. 251:1385-1395
  39. William CHJr, Kamin M (1962) Microsomal triphosphopyridine nucleotide- cytochrome C reductase of liver. J. BioI. Chem. 237:587-595
  40. Xu C, Li CY, Kong AN (2005) Induction of phase I, II and III drug metabolism/transport by xenobiotics. Arch. Pharm. Res. 28:249-268 https://doi.org/10.1007/BF02977789
  41. Yargicoglu P, Gumusluoriob S, Agar A, Korgun DK, Kucukatay V (2001) Effect of sulfur dioxide inhallation on erythrocyte antioxidant status, food intake, and lipid peroxidation during aging. Arch. Environ. Health. 56:53-57 https://doi.org/10.1080/00039890109604054
  42. You JS, Huang HF, Chang YL (2005) Pamax ginseng reduces adriamycin-induced heart failure in rats. phytother. Res. 19: 1018-1022 https://doi.org/10.1002/ptr.1778
  43. Zhou W, Chai H, Lin PH, Lumsden AB, Yao Q, Chen C (2005) Ginsneoside Rb1 blocks hemocystein-induced endothelial dysfunction in porcine coronary arteries. J. Vasc. Surg. 41:861-868 https://doi.org/10.1016/j.jvs.2005.01.054