Abstract

The aim of this work was to perform the screening and identification of the bacterium, MK-15 having the activity of fibrinolytic enzyme for the commercial use. Initially, strain MK-15 was enriched and isolated from naturally fermented soybean. Morphological and various physiological characteristics of the strain MK-15 was examined. The activity of fibrinolytic enzyme derived from supernatants of test culture MK-15 was performed by fibrin plate method for solid fibrinolytic activity. As the result, the fibrinolytic activity of MK-15 grown on the soybean media was about 2.7 times greater than that of plasmin used as stardard. 16S rRNA analyses revealed that strain MK-15 was 99.9% similar to Bacillus subtilis species cluster, and the bacterium was designated as Bacillus sp. MK-15. Strain MK-15 was registered in GenBank as [DQ163021].

본 연구의 목적은 상업적으로 사용하기 위하여 혈전용해효소를 생성하는 세균인 MK-15를 분리하고 동정하는 것이다. 먼저 세균 MK-15는 자연 발효된 콩으로부터 농화분리하였으며, 이 세균에 대한 형태학적 및 다양한 생리학적 특성 조사를 실시하였다. MK-15의 배양 상등액으로부터 혈전용해효소 활성을 fibrin plate 방법으로 solid fibrinolytic activity를 측정하였다. 그 결과, 콩배지에서 자란 MK-15의 혈전용해활성은 대조군으로 사용된 plasmin의 혈전용해활성에 비하여 약 2.5배 높았다. 16S rRNA를 분석한 결과 균주 MK-15는 Bacillus subtilis 군과 99.9% 유사성을 나타내었다. 따라서, 이 세균은 Bacillus sp. MK-15로 명명하였으며. 균주 MK-15는 GenBank에 [DQ163021]로 등록하였다.

Keywords

• Bacillus sp. MK-15

• 혈전용해효소;
• 청국장;
• 콩발효;