Journal of Asia-Pacific Entomology

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Storage Proteins of Diamondback Moth, Plutella xylostella: Purification and Expression Profile

  • Ibrahim Ahmed M.A. (Department of Agricultural Biology, Andong National University) ;
  • Lee Kee-Woo (Department of Bioelectronic Engineering, Andong National University) ;
  • Lee Hyuk-Soo (Department of Bioelectronic Engineering, Andong National University) ;
  • Kim Yong-Gyun (Department of Agricultural Biology, Andong National University)
  • Published : 2006.06.01
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Abstract

Storage proteins (SPs) were significantly detected in the hemolymph during the late instar larvae of diamondback moth, Plutella xylostella. These SPs were resolved into three proteins (SP1, SP2, and SP3) at 7% SDS-PAGE. Their apparent molecular sizes were around 80 kDa. SP1 was synthesized later than SP2 and SP3 during the development of the last instar. Total soluble proteins of last instar larvae were extracted and fractionated sequentially with ammonium sulfate, size-exclusion chromatography, and ion-exchange chromatography. The SPs were purified and their developmental expression was discussed.

Keywords

References

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