Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Rhodiola Sachalinensis Administration and Endurance Exercise on Insulin Sensitivity and Expression of Proteins Related with Glucose Transport in Skeletal Muscle of Obese Bucker Rat

홍경천 섭취와 운동수행이 비만 쥐의 인슐린 민감도와 골격근내 당수송 관련 단백질 발현에 미치는 영향

  • Oh Jae-Keun (Department of Sports Medicine, Korea National Sport University) ;
  • Shin Young-Oh (Department of Sports Medicine, Korea National Sport University) ;
  • Jung Hee-Jung (Department of Sports Medicine, Korea National Sport University) ;
  • Lee Jung-Eun (Department of Sports Medicine, Korea National Sport University)
  • 오재근 (한국체육대학교 스포츠의학실) ;
  • 신영오 (한국체육대학교 스포츠의학실) ;
  • 정희정 (한국체육대학교 스포츠의학실) ;
  • 이정은 (한국체육대학교 스포츠의학실)
  • Published : 2006.06.01
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Abstract

Peripheral insulin resistance in obese/type II diabetes animals results from an impairment of insulin-stimulated glucose uptake into skeletal muscle. Insulin stimulate the translocation of GLUT4 from intracellular location to the plasma membrane. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) is implicated in mediation of fusion of GLUT4-containing vesicle with the plasma membrane. Present study investigated regulatory effects of Rhodiola sachalinensis administration and exercise training on the expression of GLUT4 protein and SNAREs protein in skeletal muscles of obese Zucker rats. Experimental animals were randomly assigned into one of five groups ; lean control(LN), obese control(OB), exercise-treated(EXE), Rhodiola sachalinensis-treated(Rho), combine of Rho & EXE (Rho-EXE). All animals of exercise training (EXE, Rho-EXE) performed treadmill running for 8 weeks, and animals of Rho groups (Rho, Rho-EXE) were dosed daily by gastric gavage during the same period. After experiment, blood were taken for analyses of glucose, insulin, and lipids levels. Mitochondrial oxidative enzyme (citrate synthase, CS ; $\beta$-hydroxyacyl-CoA dehydrogenase, $\beta$-HAD) activity were analysed. Skeletal muscles were dissected out for analyses of proteins (GLUT4, VAMP2, syntaxin4, SNAP23). Results are as follows. Exercise and/or Rhodiola sachalinensis administration significantly reduced body weight and improved blood lipids (TG, FFA), and increased insulin sensitivity. Endurance exercise significantly increased the activity of mitochondrial enzymes and the expression of GLUT4 protein, however, administration of Rhodiola sachalinensis did not affect them. The effect of exercise and/or Rhodiola sachalinensis administration on the expression of SNARE proteins was unclear. Our study suggested that improvement insulin sensitivity by exercise and/or Rhodiola sachalinensis administration in obese Zucker rats is independent of expression of SNARE proteins.

Keywords

References

  1. Diabetes, 2nd revision, pp.239, 233-234, 335-343, Korean Diabetes Association, Seoul, 1998
  2. Lee BY. Physiology, 6th revision, pp.93-94, 330-331, Shinkwang publisher, Seoul, 2000
  3. Douen AG, Ramlal T, Klip A, Young DA, Cartee GD, Holloszy JO. Exercise-induced increase in glucose transporters in plasma membranes of rat skeletal muscle. Endocrinology 124(1): 449- 454, 1989 https://doi.org/10.1210/endo-124-1-449
  4. Hirshman MF, Goodyear LJ, Wardzala LJ, Horton ED, Horton ES. Identification of an intracellular pool of glucose transporters from basal and insulin-stimulated rat skeletal muscle. J Biol Chem 265(2): 987-991, 1990
  5. Daugaard JR, Richter EA. Relationship between muscle fibre composition, glucose transporter protein 4 and exercise training: possible consequences in non-insulin-dependent diabetes mellitus. Acta Physiol Scand 171(3): 267-276, 2001 https://doi.org/10.1046/j.1365-201x.2001.00829.x
  6. Foster LJ, Klip A. Mechanism and regulation of GLUT-4 vesicle fusion in muscle and fat cells. Am J Physiol Cell Physiol 279(4): C877-890, 2000 https://doi.org/10.1152/ajpcell.2000.279.4.C877
  7. Malhotra V, Orcl L, Glick BS, Block MR, Rothman JE. Role of N-ethylmaleimide -sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack. Cell 54:221-227, 1988 https://doi.org/10.1016/0092-8674(88)90554-5
  8. James DJ, Cairns F, Salt IP, Murphy GJ, Dominiczak AF, Connell JM, Gould GW. Skeletal muscle of stroke-prone spontaneously hypertensive rats exhibits reduced insulin-stimulated glucose transport and elevated levels of caveolin and flotillin. Diabetes 50(9): 2148-2156, 2001 https://doi.org/10.2337/diabetes.50.9.2148
  9. Maier VH, Melvin DR, Lister CA, Chapman H, Gould GW, Murphy GJ. v- and t-SNARE protein expression in models of insulin resistance: normalization of glycemia by rosiglitazone treatment corrects overexpression of cellubrevin, vesicle-associated membrane protein-2, and syntaxin 4 in skeletal muscle of Zucker diabetic fatty rats. Diabetes 49(4): 618-625, 2000 https://doi.org/10.2337/diabetes.49.4.618
  10. 김철현, 임예현, 조인호, 김성찬, 방상식, 조준용, 이규성. 카테킨 섭취와 운동수행에 따른 obese zucker rat 골격근의 GLUT-4, VAMP-2와 Syntaxin-4 단백질 발현의 변화. 운동과학 12(4): 663-678, 2003
  11. Chem X, Di L, Wu Y, Liu X, Ren Q. Hypoglycemic effect of Rhodiola sachalinensis A. Bor. polysaccharides: comparison of administration in different ways. Zhongguo Zhong Yao Za Zhi 21(11): 685-687, 1996
  12. Cheng XJ, Di L, Wu Y, Zhao QC, Du GZ, Liu YQ. Studies on the hypoglycemic effect of Rhodiola sachalinensis A. Bor. polysaccharides. Zhongguo Zhong Yao Za Zhi 18(9): 557-559, 575, 1993
  13. Molokovskii DS, Davydov VV, Tiulenev VV. The action of adaptogenic plant preparations in experimental alloxan diabetes. Probl Endokrinol (Mosk) 35(6): 82-87, 1989
  14. Bray GA. The Zucker-fatty rat: a review. Fed Proc 36(2): 148- 153, 1977
  15. Zucker LM. Fat mobilization in vitro and in vivo in the genetically obese Zucker rat 'fatty'. J Lipid Res 13(2): 234-243, 1972
  16. Krssak M, Falk Petersen K, Dresner A, DiPietro L, Vogel SM, Rothman DL, Roden M, Shulman GI. Intramyocellular lipid concentrations are correlated with insulin sensitivity in humans: a 1H NMR spectroscopy study. Diabetologia 42(1): 113-116, 1999 https://doi.org/10.1007/s001250051123
  17. Pan DA, Lillioja S, Kriketos AD, Milner MR, Baur LA, Bogardus C, Jenkins AB, Storlien LH. Skeletal muscle triglyceride levels are inversely related to insulin action. Diabetes 46(6): 983-988, 1997 https://doi.org/10.2337/diabetes.46.6.983
  18. Perseghin G, Scifo P, De Cobelli F, Pagliato E, Battezzati A, Arcelloni C, Vanzulli A, Testolin G, Pozza G, Del Maschio A, Luzi L. Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans: a 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents. Diabetes 48(8): 1600-1606, 1999 https://doi.org/10.2337/diabetes.48.8.1600
  19. Delp MD, Duan C. Composition and size of type I, IIA, IID/X, and IIB fibers and citrate synthase activity of rat muscle. J Appl Physiol 80(1): 261-270, 1996 https://doi.org/10.1152/jappl.1996.80.1.261
  20. Leek BT, Mudaliar SR, Henry R, Mathieu-Costello O, Richardson RS. Effect of acute exercise on citrate synthase activity in untrained and trained human skeletal muscle. Am J Physiol Regul Integr Comp Physiol 280(2): R441-447, 2001 https://doi.org/10.1152/ajpregu.2001.280.2.R441
  21. Zonderland ML, Bar PR, Reijneveld JC, Spruijt BM, Keizer HA, Glatz JF. Different metabolic adaptation of heart and skeletal muscles to moderate-intensity treadmill training in the rat. Eur J Appl Physiol Occup Physiol 79(5): 391-396, 1999 https://doi.org/10.1007/s004210050527
  22. Bruce CR, Kriketos AD, Cooney GJ, Hawley JA. Disassociation of muscle triglyceride content and insulin sensitivity after exercise training in patients with Type 2 diabetes. Diabetologia 47(1): 23-30, 2004 https://doi.org/10.1007/s00125-003-1265-7
  23. Dela F, Ploug T, Handberg A, Petersen LN, Larsen JJ, Mikines, KJ, Galbo H. Physical training increases muscle GLUT4 protein and mRNA in patients with NIDDM. Diabetes 43(7): 862-865, 1994 https://doi.org/10.2337/diabetes.43.7.862
  24. Pedersen O, Bak JF, Andersen PH, Lund S, Moller DE, Flier JS, Kahn BB. Evidence against altered expression of GLUT1 or GLUT4 in skeletal muscle of patients with obesity or NIDDM. Diabetes 39(7): 865-870, 1990 https://doi.org/10.2337/diabetes.39.7.865
  25. Gan SK, Kriketos AD, Ellis BA, Thompson CH, Kraegen EW, Chisholm DJ. Changes in aerobic capacity and visceral fat but not myocyte lipid levels predict increased insulin action after exercise in overweight and obese men. Diabetes Care 26(6): 1706-1713, 2003 https://doi.org/10.2337/diacare.26.6.1706
  26. Goodpaster BH, Katsiarasm A, Kelley DE. Enhanced fat oxidation through physical activity is associated with improvements in insulin sensitivity in obesity. Diabetes 52(9): 2191-2197, 2003 https://doi.org/10.2337/diabetes.52.9.2191
  27. Banks EA, Brozinick JT Jr, Yaspelkis BB 3rd, Kang HY, Ivy JL. Muscle glucose transport, GLUT-4 content, and degree of exercise training in obese Zucker rats. Am J Physiol 263: E1010-1015, 1992
  28. Brozinick JT Jr, Etgen GJ Jr, Yaspelkis BB 3rd, Kang HY, Ivy JL. Effects of exercise training on muscle GLUT-4 protein content and translocation in obese Zucker rats. Am J Physiol 265: E419-427, 1993
  29. Ivy JL, Sherman WM, Cutler CL, Katz AL. Exercise and diet reduce muscle insulin resistance in obese Zucker rat. Am J Physiol 251(3): E299-305, 1986
  30. Ivy JL, Brozinick JT Jr, Torgan CE, Kastello GM. Skeletal muscle glucose transport in obese Zucker rats after exercise training. J Appl Physiol 66(6): 2635-2641, 1989 https://doi.org/10.1152/jappl.1989.66.6.2635
  31. Ivy JL. Muscle insulin resistance amended with exercise training: role of GLUT4 expression. Med Sci Sports Exerc 36(7): 1207- 1211, 2004
  32. Rothman JE. Mechanisms of intracellular protein transport. Nature 372(6501): 55-63, 1994 https://doi.org/10.1038/372055a0
  33. Sollner T, Whiteheart SW, Brunner M, Erdjument-Bromage H, Geromanos S, Tempst P, Rothman JE. SNAP receptors implicated in vesicle targeting and fusion. Nature 362(6418): 318-324, 1993 https://doi.org/10.1038/362318a0
  34. Sudhof TC, De Camilli P, Niemann H, Jahn R.. Membrane fusion machinery: insights from synaptic proteins. Cell 75(1): 1-4, 1993 https://doi.org/10.1016/S0092-8674(05)80077-7
  35. King PA, Horton ED, Hirshman MF, Horton ES. Insulin resistance in obese Zucker rat (fa/fa) skeletal muscle is associated with a failure of glucose transporter translocation. J Clin Invest 90(4): 1568-1575, 1992 https://doi.org/10.1172/JCI116025
  36. Garvey WT, Maianu L, Zhu JH, Brechtel-Hook G, Wallace P, Baron AD. Evidence for defects in the trafficking and translocation of GLUT4 glucose transporters in skeletal muscle as a cause of human insulin resistance. J Clin Invest 101(11): 2377-2386, 1998 https://doi.org/10.1172/JCI1557
  37. Perez-Martin A, Raynaud E, Mercier J. Insulin resistance and associated metabolic abnormalities in muscle: effects of exercise. Obes Rev 2(1): 47-59, 2001 https://doi.org/10.1046/j.1467-789x.2001.00024.x
  38. Omata W, Shibata H, Li L, Takata K, Kojima I. Actin filaments play a critical role in insulin-induced exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes. Biochem J 346(2): 321-328, 2000 https://doi.org/10.1042/0264-6021:3460321
  39. Valentijn JA, Valentijn K, Pastore LM, Jamieson JD. Actin coating of secretory granules during regulated exocytosis correlates with the release of rab3D. Proc Natl Acad Sci USA 97(3): 1091- 1095, 2000
  40. Srere PA. Citrate synthase. Methods Enzymol 13: 3-11, 1969 https://doi.org/10.1016/0076-6879(69)13005-0
  41. Bass A, Brdiczka DS, Pette D. Metabolic differentiation of distinct muscle types at the level of enzymatic organization. Eur J Biochem 10: 198-206, 1969 https://doi.org/10.1111/j.1432-1033.1969.tb00674.x
  42. 中藥大辭典. pp.107-111, 上海人民出版社, 1975
  43. Chung TH. Korean Flora (Herb part). pp.283, 1974
  44. Lee YN. Flora of Korea. pp.277, Kyohak, Seoul, 1997
  45. Lee YA, Cho SM, Lee MW. Flavonoids from the roots of Rhodiola sachalinensis. Kor J Pharmacogn 33(2): 116-119, 2002