Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Coenzyme $Q_{10}$ by Recombinant E. coli Harboring the Decaprenyl Diphosphate Synthase Gene from Sinorhizobium meliloti

  • Seo Myung-Ji (Division of Bio-New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Im Eun-Mi (Division of Bio-New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Hur Jin-Haeng (Division of Bio-New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Nam Jung-Yeon (Department of Biotechnology, Yonsei University) ;
  • Hyun Chang-Gu (Division of Bio-New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Pyun Yu-Ryang (Department of Biotechnology, Yonsei University) ;
  • Kim Soon-Ok (Division of Bio-New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI))
  • Published : 2006.06.01
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Abstract

Decaprenyl diphosphate synthase (DPS) is the key enzyme for the production of coenzyme $Q_{10}$ ($CoQ_{10}$). A dps gene from Sinorhizobium meliioti KCCM 11232 (IFO 14782) was isolated by PCR and then cloned in Escherichia coli. DNA sequencing analysis revealed an open reading frame of 1,017 bp encoding a 338-amino-acid protein. The protein was identical at the 98% level to the putative octaprenyl diphosphate synthase (IspB) of S. meliloti 1021. The deduced amino acid sequence included the DDxxD domains conserved in the majority of the prenyl diphosphate synthases. Heterologous expression in E. coli BL21 (DE3) was carried out, and the $CoQ_{10}$ produced was then analyzed by HPLC. E. coli BL21 (DE3) harboring the dps gene from S. melioti produced CoQ$_{10}$ in addition to endogenous coenzyme Q$_8$ (CoQ$_8$), whereas wild-type E. coli BL21 (DE3) host did not have the ability of producing CoQ$_{10}$. The results suggest that the putative dps from S. meliloti KCTC 2353 encoded the DPS.

Keywords

References

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