Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Isolation and characterization of Clostridium Perfringens on bovine and porcine carcass

소와 돼지의 도체에서 Clostridium perfringens의 분리와 특성

  • Chae Hee-Sun (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim Youn-Ha (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim Ju-Young (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim Jong-Hwa (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim Gyu-Hyeon (Seoul Metropolitan Health & Environment Research Institute) ;
  • Choi Tae-Seok (Seoul Metropolitan Health & Environment Research Institute) ;
  • Shin Bang-Woo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee Duck-Joo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee Jung-Hark (Seoul Metropolitan Health & Environment Research Institute)
  • 채희선 (서울특별시 보건환경 연구원) ;
  • 김연하 (서울특별시 보건환경 연구원) ;
  • 김주영 (서울특별시 보건환경 연구원) ;
  • 김종화 (서울특별시 보건환경 연구원) ;
  • 김규현 (서울특별시 보건환경 연구원) ;
  • 최태석 (서울특별시 보건환경 연구원) ;
  • 신방우 (서울특별시 보건환경 연구원) ;
  • 이덕주 (서울특별시 보건환경 연구원) ;
  • 이정학 (서울특별시 보건환경 연구원)
  • Published : 2006.06.01
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Abstract

A total of 1,848 samples was taken from bovine and porcine from January 2003 to November 2005. They were examined for the presence of Clostridiuml perfringens. The properties of the isolates were characterized for Gram staining, biochemical features and enterotoxin production. Forty-one strains (2.2%) of C perfringens were isolated from the 1,848 bovine and porcine carcass using selective media. 30 (3.2%) C perfringens were isolated from the 925 of bovine cacasses, and 11(1.2%) were isolated from the 923 of porcine cacasses. In TSC agar, all isolates showed lecithinase activity. The isolation rate was higher in spring and summer than in autumn and winter. Among 41 isolates, only 1 isolate detected the cpe gene in PCR. The PCR amplified band was observed 233 bp.

Keywords

References

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