The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

The Study on the Effectiveness and Mechanism of Several Herbal Medicines for Development of Osteoarthritis Treatment

퇴행성관절염(退行性關節炎) 치료제 개발을 위한 수종의 한약재활성 검색 및 기전연구

  • Huh Jeong-Eun (Oriental Medicine Research Center for Bone & Joint Disease) ;
  • Cho Eun-Mi (Oriental Medicine Research Center for Bone & Joint Disease) ;
  • Yang Ha-Ru (Oriental Medicine Research Center for Bone & Joint Disease) ;
  • Kim Dae-Sung (Oriental Medicine Research Center for Bone & Joint Disease) ;
  • Baek Yong-Hyeon (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung-Hee University) ;
  • Lee Jae-Dong (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung-Hee University) ;
  • Choi Do-Young (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung-Hee University) ;
  • Park Dong-Suk (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung-Hee University)
  • 허정은 (경희대학교 골관절질환 한방연구센터) ;
  • 조은미 (경희대학교 골관절질환 한방연구센터) ;
  • 양하루 (경희대학교 골관절질환 한방연구센터) ;
  • 김대성 (경희대학교 골관절질환 한방연구센터) ;
  • 백용현 (경희대학교 부속한방병원 침구학교실) ;
  • 이재동 (경희대학교 부속한방병원 침구학교실) ;
  • 최도영 (경희대학교 부속한방병원 침구학교실) ;
  • 박동석 (경희대학교 부속한방병원 침구학교실)
  • Published : 2006.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracelluar matrix. The purpose of this study was to investigate the effects of KHBJs for cartilage-protective effect in human and rabbit articular cartilage explants. Methods : The cartilage-protective effects of KHBJ were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMPs activity, and histological analysis in rabbit and human cartilage explants culture. Results : KHBJs significantly inhibited GAG and collagen release of rabbit and human cartilage explant in a concentration-dependent manner. Also, KHBJs inhibited MMP-3 and MMP-13 activities from IL-$1{\alpha}$-treated cartilage explants cultures. Histological analysis indicated that KHBJ004 reduced the degradation of the cartilage matrix compared with that of IL-$1{\alpha}$-treated cartilage explants. KHBJ004 had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Conclusions : These results indicate that KHBJs inhibits the degradation of proteoglycan and collagen through the downregulation of MMP-3 and MMP-13 activities without affecting the viability or morphology of IL-$1{\alpha}$-stimulated rabbit and human articular cartilage explants.

Keywords