Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

A Study on the Localization of Neurofascin in the Myelinated Rat Sciatic Nerve Fibers

랫드 수초좌골신경섬유에서 Neurofascin분포에 대한 면역세포화학적 연구

  • Chang, Byung-Hwa (Electron Microscope Facility, College of Medicine, Konkuk University) ;
  • You, Kwan-Hee (School of Bioscience & Biotechnology, Chungnam National University) ;
  • Lee, Jong-Hwan (College of Veterinary Medicine, Konkuk University) ;
  • Cho, Ik-Hyun (College of Veterinary Medicine, Konkuk University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine, and Biotechnology Research Institutes, Chonnam National University) ;
  • Park, Chang-Hyun (Electron Microscope Facility, College of Medicine, Korea University) ;
  • Han, Jeong-Mi (Electron Microscope Facility, College of Medicine, Ewha Woman's University) ;
  • Choe, Nong-Hoon (College of Veterinary Medicine, Konkuk University) ;
  • Chang, Byung-Joon (College of Veterinary Medicine, Konkuk University)
  • 장병화 (건국대학교 의과대학 전자현미경실) ;
  • 유관희 (충남대학교 생명과학부) ;
  • 이종환 (건국대학교 수의과대학) ;
  • 조익현 (건국대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 박창현 (고려대학교 의과대학 전자현미경실) ;
  • 한정미 (이화여자대학교 의과대학 전자현미경실) ;
  • 최농훈 (건국대학교 수의과대학) ;
  • 장병준 (건국대학교 수의과대학)
  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the myelination of peripheral nerves, the localization of neurofascin in myelinated rat sciatic nerve fibers was studied with the immuno-fluorescence and immune-electron microscopy and the results are as follows; 1. According to the myelination is going on, neurofascin localization was dramatically changed in the sciatic nerve fibers. 2. In the myelinated fibers, neurofascin was weakly localized along the axolemma at the node of Ranvier. 3. Neurofascin was also apparantly localized at the non-compact area of Schwann cell membrane such as paranodal loop, Schmidt-Lantermann incisure, inner & outer mesaxons in the myelinated fibers. From the above results, neurofascin is likely to have a role to sustain the ideal gap of apposing membranes of Schwann cell, so it may enable to materials transport in the myelin sheath.

Neurofascin은 L1CAM의 하나로 신경섬유의 발달과정에서 중요한 역할을 하는 것으로 알려져 있다. 말초신경의 수초형성과 관련된 neurofascin의 역할을 알아볼 목적으로 면역형광염색과 면역전자현미경기법을 이용하여 랫드의 수초좌골신경섬유에서 neurofascin의 분포를 추구하여 다음과 같은 결과를 얻었다. 1.수초형성이 진행됨에 따라 좌골신경섬유에서 neurofascin 분포는 매우 심하게 변화되었다. 2. 수초신경섬유에서 neurofascin은 Ranvier마디에서 약하게 국재하였다. 3. Neurofascin은 수초신경섬유의 paranodal loop, Schmidt-Lantermann incisure, 속축삭사이막, 바깥축삭사이막처럼 Schwann세포의 막이 밀착되지 않은 부위에서도 뚜렷하게 국재하였다. 이상의 연구결과로 neurofascin은 Schwann세포의 마주보는 막사이에 이상적인 간격을 유지하는데 어떤 역할을 하는 것으로 생각되며, 수초층에서 물질이동이 가능하게 하는 것으로 보인다.

Keywords

References

  1. Cunningham BA: Cell adhesion molecules as morphoregulators. Curr Opin Cell Biol 7 : 628-633, 1995 https://doi.org/10.1016/0955-0674(95)80103-0
  2. Grumet M, Mauro V, Burgoon MP, Edelman GM, Cunningham BA: Structure of a new nervous system glycoprotein, Nr-CAM, and its relationship to subgroups of neural cell adhesion molecules. J Cell Biol 113 : 1399-1412, 1991 https://doi.org/10.1083/jcb.113.6.1399
  3. Hortsch M: The L1 family of neuronal cell adhesion molecules: Old proteins performing new tricks. Neuron 17 : 587-593, 1995 https://doi.org/10.1016/S0896-6273(00)80192-0
  4. Rathjen FG, Schachner M: Immunocytochemical and biolochemical characterization of a new neuronal cell-surface component (L1-antigen) which is involved in cell-adhesion. EMBO 3 : 1-10, 1984 https://doi.org/10.1093/embo-reports/kvf015
  5. Volkmer H, Hassel B, Wolff JM, Frank R, Rathjen FG: Structure of the axonal surface recognition molecule neurofascinand its relationship to a neural subgroup of the immunoglobulin superfamily. J Cell Biol 118 : 149-161, 1992 https://doi.org/10.1083/jcb.118.1.149
  6. Rathjen FG, Wolff JM, Chang S, Bonhoeffer F, Raper JA: Neurofascin: A novel chick cell-surface glycoprotein involved in neurite-neurite interactions. Cell 51 : 841-849, 1987 https://doi.org/10.1016/0092-8674(87)90107-3
  7. Shiga T, Oppenheim RW: Immunolocalization studies of putitive guidance molecules used by axons and growth cones of intersegmental interneurons in the chick embryo spinal cord. J Comp Neurol 310 : 234-252, 1991 https://doi.org/10.1002/cne.903100208
  8. Moscoso LM, Sanes JR: Expression of 4 immunoglobulin superfamily adhesion molecules (L1, Nr-CAM/Bravo, neurofascin/ABGP, and N-CAM) in the developing mouse spinal cord. J Comp Neurol 352 : 321-334, 1995 https://doi.org/10.1002/cne.903520302
  9. Davis JQ, Lambert S, Bennett V: Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FN III domain-) and NrCAM at nodal axon segments. J Cell Biol 135 : 1355-1367, 1996 https://doi.org/10.1083/jcb.135.5.1355
  10. Davis JQ, Bennett V: Ankyrin-binding activity shared by the neurofascin/L1/NrCAM family of nervous system cell adhesion molecules. J Biol Chem 269 : 27163-27166, 1994
  11. Davis JQ, McLaughlin T, Bennett V: Ankyrin-binding proteins related to nervous system cell adhesion molecules: candidates to provide transmembrane and intracellular connections in adult brain. J Cell Biol 121 : 121-133, 1993 https://doi.org/10.1083/jcb.121.1.121
  12. Dubreuil RR, MacVicar G, Dissanayake S, Liu C, Homer D, Hortsch M: Neuroglian-mediated cell adhesion induces assembly of the membrane skeleton at cell contact sites. J Cell Biol 133 : 647-655, 1996 https://doi.org/10.1083/jcb.133.3.647
  13. Collinson JM, Marshall D, Gillespie CS, Brophy PJ: Transient expression of neurofascin by oligodendrocytes at the onset of myelinogenesis: Implications for mechanisms of axon-glial interaction. Glia 23 : 11-23, 1998 https://doi.org/10.1002/(SICI)1098-1136(199805)23:1<11::AID-GLIA2>3.0.CO;2-7
  14. Tait S, Gunn-More F, Collinson JM, Huang J, Lubetzki C, Pedraza L, Sherman DL, Colman DR, Brophy PJ: An oligodendrocyte cell adhesion molecule at the site of assembly of the paranodal axo-glial junction. J Cell Biol 150(3) : 657-666, 2000 https://doi.org/10.1083/jcb.150.3.657
  15. Cooper NA, Kidman AD: Quantitation of the Schmidt-Lantermann incisures in juvenile, adult, remyelinated and regenerated fibers of the chicken sciatic nerve. Acta Neuropathol 64 : 251-258, 1984 https://doi.org/10.1007/BF00688116
  16. Gabriel MN, Allt G: Incisures of Schmidt-Lanterman. Prog Neubiol 17 : 25-58, 1981 https://doi.org/10.1016/0301-0082(81)90003-4
  17. Krinke G, Grieve AP, Schnider K: The roleof Schmidt-Lanterman incisures in Wallerian degeneration. Acta Neurophathol 69 : 168-170, 1986 https://doi.org/10.1007/BF00687055
  18. Reynolds RJ, Heath JW: Patterns of morphological variation within myelin internodes of normal peripheral nerve: quantitative analysis by confocal microscopy. J Anat 187 : 369-378, 1995
  19. Small JR, Ghabriel MN, Allt G: The development of Schmidt-Lantermann incisures: an electron microscope study. J Anat 150 : 277-286, 1987