Antioxidant and Antimicrobial Activities of Cambodian Mushroom, Phellinus linteus

상황버섯 (Phellinus linteus)으로부터 항산화 및 항균 효과

  • Kim In-Hae (Department of Pharmaceutical Engineering, Collage of Medical Life Science, Silla University) ;
  • Jin Eun-Jung (Department of Bioscience and Biotechnology, Collage of Engineering, Silla University) ;
  • Lee Jae-Hwa (Department of Pharmaceutical Engineering, Collage of Medical Life Science, Silla University)
  • 김인혜 (신라대학교 의생명과학대학 제약공학과) ;
  • 진은정 (신라대학교 공과대학 생명공학과) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과)
  • Published : 2006.06.01

Abstract

This study was peformed to determine the antioxidant, antimicrobial activity and antifungal of the cambodian mushroom, Phellinus linteus, in various concentrations from the mushroom extracts. It's extracts were found to cause significant free radical scavenging effects on DPPH (1,1-diphenyl-2-picrylhydrazyl). This extracts exhibited in vitro broad-spectrum antimicrobial activity of Gram-negative, Gram-positive bacteria and without antifungal activity. On the other hand, the extracts had not significant hemolytic activity against human red blood cells. These results indicate cambodian mushroom, Phellinus inteus having antioxidant and antimicrobial activity without hemolytic activity maybe useful as therapeutic agents.

Keywords

References

  1. Gibson, B.W., Tang, D.Z., Mandrell, R., Kelly, M. and Spindel, R. R. (1991). Bombinin-like peptides with antimicrobial activity from skin secretions of asian toad, Bomina orientails. J.Biol. Chem., 266, 23103-23111
  2. Han, S.-B., Lee, C.-W., Jeon, Y.-J. (1999). The inhibitory effect of polysaccharide isolated from Phellinus linteus on tumour growth and metastasis. Immunpharmacology, 41, 157-164 https://doi.org/10.1016/S0162-3109(98)00063-0
  3. Ji, J.-H., Kim, M.-N., Chung, C.-K. and Han, S.-S. (2000) Antimutagenic and cytotoxicity effects of Phellinus linteus Extracts. J. Koream Soc. Food Sci. Nutr., 29, 322-328
  4. Kim, H.M., Han, S.B., Oh, G.T., Kim, Y.H., Hong, D.H., Hong, N.D. and Yoo, I.D. (1996) Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int. J. Immunopharmacol. 18, 295-303 https://doi.org/10.1016/0192-0561(96)00028-8
  5. Kitagaki, H. and Tsugawa, M. (1999) 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavanging ability of sake during storage. J. Biosci. Bioeng., 87, 328-332 https://doi.org/10.1016/S1389-1723(99)80040-1
  6. Koleva, I.I., Van Bevek, T.A. and linssen, J.P.H. (2002) Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem. Anal., 13, 8-17 https://doi.org/10.1002/pca.611
  7. Lee, H.J., Lee, H.J., Park, J.M., Song, G.Y., Kang, K.S. and Kim, S.H. (2002) Study on antitumor and limmuno-regulatory effects on cambodian Phenolions linteus. Korean J. Oreiental Physiology & Pathology, 16, 332-337
  8. Lee, J.H., Cho, S.M., Song, K.S., Hong, N.D. and Yoo, I.D. (1996) Characterization of carbohydrate-peptide linkage of acidic heteroglycopeptide with immunostimulating activity from mycelium of Phellinus linteus. Chem. Pharm. Bull (Tokyo), 43, 2105-2108
  9. Lehrer, R.I., Rosenman, M., Harwig, S.S., Jackson, R. and Eisenhauer, P. (1991) Ultrasensitive assays for endogenous antimicrobial polypeptides. J. Immunol. Methods, 137, 167-173 https://doi.org/10.1016/0022-1759(91)90021-7
  10. Park, N.G., Yamato, Y., Lee, S.M., Sugihara, G., Park, J.-S. and Kang, S.-W., The interaction of mastoparan B from venom of hornet Vespa basalis with phospholipid matrices. (1996) Bull. Korean Chem. Soc., 17, 50-56
  11. Sung, J.M., Yoo, Y.B. and Cha, D.Y. (1998) Mushroom kyohaksa, Seoul, 3
  12. Yamada, S. (1994) Mycelial culture method of Phellinus linteus. Korean patient 92-1194