DOI QR코드

DOI QR Code

Response of Several Fungicides of Colletotrichum gloeosporioides Isolates Obtained from Persimmons in Sangju

상주 지역 감나무로부터 분리한 Colletotrichum gloeosporioides 균주들의 방제 약제에 대한 반응

  • Lim Tae-Heon (Technology Innovation Center, Sangju National University) ;
  • Choi Yong-Hwa (Department of Plant Resources, Sangju National University)
  • 임태헌 (상주대학교 지역기술혁신센터) ;
  • 최용화 (상주대학교 생명과학대학 식물자원학과)
  • Published : 2006.08.01

Abstract

In 2005, 90 isolates of Colletotrichum gloeosporioides causing persimmons tree anthracnose were obtained from infected twigs and fruits of persimmon trees. Their responses to nine fungicides, consisting of two benzimidazoles (thiophanate-methyl, carbendazim), three ergosterol-biosynthesis inhibitors (difenoconazole, myclobutanil, tebuconazole), and four protective fungicides (propineb, mancozeb, chlothalonil, and dithianon), were investigated with relative mycelial growth index to untreated control on PDA treated with field application rate of each fungicide. At response to carbendazim ($415{\mu}g/ml$) and thiophanate-methyl ($750{\mu}g/ml$), 82% and 78% of isolates showed relative mycelial growth index under 0.1 to untreated control, respectively. All of them did not grow on PDA incorporated with myclobutanil ($40{\mu}g/ml$) and tebuconazole ($75{\mu}g/ml$). Only one isolate (PER 36) grew on PDA amended with difenoconazole ($50{\mu}g/ml$), but its relative mycelial growth index to untreated control was very low with a values of 0.03. They were most sensitive to propineb ($1,500{\mu}g/ml$) among four protective fungicides.

상주 지역의 곶감용 감 생산 농가의 과수원으로부터 채집한 탄저병 감염 부위로부터 90 개의 감 탄저병균 (Colletotrichum gloeosporioides)을 분리하였다. 두 종의 benzimidazole계 약제 (thiophanate-methyl, carbendazim), 3 종의 egosterol 합성 저해제 (difenoconazole, myclobutanil, tebuconazole) 및 4 종의 보호 살균제 (propineb, mancozeb, chlorothalonil, dithianon) 등 총 9 종의 살균제에 대하여 분리한 균주들의 약제반응을 조사하였다. 상용농도로 약제가 첨가된 배지에서 대조구 대비 균사생육지수를 평가함으로서 조사하였다. Carbendazim($415{\mu}g/ml$)과 thiophanate-methyl($750{\mu}g/ml$) 에 대한 반응은 각각 82%와 78%의 균주가 0.1 이하의 대조구 대비 균사생육지수를 보였다. 모든 실험 대상 균주는 myc1obutanil($40{\mu}g/ml$)과 tebuconazole ($75{\mu}g/ml$)이 첨가된 배지에서 균사생육이 완전히 억제되었으며, difenoconazole($50{\mu}g/ml$)이 첨가 배지에서는 단지 l 균주만이 0.03 의 대조구 대비 균사생장지수를 보였다. 4 종의 보호 살균제 중 propineb($1,500{\mu}g/ml$) 에 대한 감수성이 가장 높게 나타났다.

Keywords

References

  1. Adaskaveg, J. E. and Forsyer, H. 2000. Occurrence and management of anthracnose epidemics caused by Colletotrichum species on tree fruits crops in California. In: Colletotrichum-Host Specificity, Pathology and Host-Pathogen Interaction, ed. by Prusky, D., Freeman, S. and Dickman, pp. 317-336. APS Press, Minnesota. USA
  2. Delp, C. J. 1988. Fungicide Resistance in North America. The American Phytopathological Society, St. Paul, Mn. 133 pp
  3. Eckert, J. W., Sievert, J. R. and Ratnayake, M. 1994. Reduction of imazalil effectiveness against citrus gray mold in California packinghouses by resistant biotypes of Penicillium digitatum. Plant Dis. 78: 971-974 https://doi.org/10.1094/PD-78-0971
  4. Fry, W. E. 1982. Principles of Plant Disease Management. Academic Press, Orlando. 378 pp
  5. Guan, J. and de Warrd, M. A. 1993. Inhibition of sterol 14-demethylase activity in Penicillium italicum dose not collect with resistance to the DMI fungicide imazalili. Pestci. Biochem. Physiol. 46: 1-6 https://doi.org/10.1006/pest.1993.1030
  6. Hildebrand, P. D., Lockart, C. L., Newbery, R. J. and Ross, R. G. 1988. Resistance of Venturia inaequalis to bitertanol and other demethylation-inhibiting fungicides. Can. J. Plant Pathol. 10: 311-316 https://doi.org/10.1080/07060668809501704
  7. Jones, A. L. and Ehret, G. R. 1976. Isolation and characterization of benomyl-tolerant strains of Monilinia fructicola. Plant Dis. Reptr. 60: 765-769
  8. Kim, B. S., Lim, T. H., Park, E. W. and Cho, K. Y. 1995. Occurrence of multiple resistant isolates of Botrytis cinerea to benzimidazole and N-phenylcarbamate fungicides. Korean. J. Plant Pathol. 11: 146-150
  9. 김태춘, 이규철, 이용문. 2002 감의 생리생태와 재배신기술. 중앙생활사. 332 pp
  10. Koller, W. and Wilcox, W. F. 2001. Evidence for the predisposition of fungicide-resistance isolates of Venturia inaequalis to a preferential selection for resistance to other fungicides. Phytopathology 91: 776-781 https://doi.org/10.1094/PHYTO.2001.91.8.776
  11. Lim, T. H., Chang, T. H. and Cha, B. J. 1998. Incidence of benzimidazole- and dicarboximide-resistant isolates of Monilinia fructicola from overwintering mummies and peduncles on peach trees. Korean. J. Plant Pathol. 14: 367-370
  12. 농림수산부. 2004. 농림통계. http://www.maf.go,kr/index.jsp
  13. 상주시. 2004. 상주시 통계연보. p. 459
  14. Yasunori, T. and Hideo, I. 1998. Reduced sensitivity to fenarimol in Japanese field strains of Venturia nashicola. Pestic. Sci. 54: 150-156 https://doi.org/10.1002/(SICI)1096-9063(1998100)54:2<150::AID-PS797>3.0.CO;2-A

Cited by

  1. Biocontrol of Anthracnose of Chili Pepper by Bacillus sp. NAAS-1 vol.40, pp.4, 2012, https://doi.org/10.4489/KJM.2012.40.4.277