Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Development of simultaneous determination of vitamin A and E in infant formula by micro-HPLC

Micro-HPLC를 이용한 조제분유 중 비타민 A.E 동시분석법 개발

  • Yun I-Ran (Gyeongnam Livestock Promotion Research Institute) ;
  • Choi You-Jeong (Gyeongnam Livestock Promotion Research Institute) ;
  • Lee Min-Kwon (Gyeongnam Livestock Promotion Research Institute) ;
  • Jeong Myeong-Ho (Gyeongnam Livestock Promotion Research Institute) ;
  • Kim Byeong-Hun (Gyeongnam Livestock Promotion Research Institute)
  • 윤이란 (경상남도 축산진흥연구소) ;
  • 최유정 (경상남도 축산진흥연구소) ;
  • 이민권 (경상남도 축산진흥연구소) ;
  • 정명호 (경상남도 축산진흥연구소) ;
  • 김병훈 (경상남도 축산진흥연구소)
  • Published : 2006.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Semi-micro-HPLC using a column-switching technique was developed for simultaneous determination of vitamin A and E contents in infant formula. Vitamin A and E were extracted by PDA - HPLC with reversed phase column using organic solvent and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with hydrolysis method and rapid extraction. Developed method has many advantages of simple and rapid sample preparation and simultaneous determination of vitamin A and E by micro-HPLC using reversed phase column.

Keywords

References

  1. Wills RB, Shaw CG, Day WR. 1977. Analysis of water soluble vitamins by high pressure liquid chromatography. J Chromatogr Sci 15: 262-262
  2. The Korean Nutrition Society. 2001. Recommended dietary allowances for Koreans. 7 eds. The Korea Nutrition Society, Seoul : 2
  3. Korea Food and Drug Administration. 2005. Food Standards Codex. Korean Foods Industry Association, Seoul : 950-965
  4. AOAC. 2000. Official methods of analysis of AOAC international. 17 eds. AOAC, Gaithersbrug, MD, USA, 45: 4-5. 50: 1-5
  5. Korea Food and Drug Administration. 2003. Food Code. Korean Foods Industry Association, Seoul: 894-897, 912- 915
  6. Wickroski AF, McLean LA. 1984. Improved reverse phase liquid chromatographic determination of vitamins A and D in fortified milk. J Assoc Off Anal Chem 67 (1) : 62-65
  7. Indyk HE. 1988. Simplified saponification procedure for the routine determination of total vitamin E in dairy products, foods and tissues by high-performance liquid chromatography. Analyst 113 (8) : 1217-1221 https://doi.org/10.1039/an9881301217
  8. Albala-Hurtado S, Novella-Rodriguez S, Veciana- Nogues MT, et al. 1977. Determination of vitamins A and E in infant milk formulae by high-perfor-mance liquid chromatography. J Chromatogr A 778(1-2): 243-246 https://doi.org/10.1016/S0021-9673(97)00353-1
  9. De Vries JW, Silvera KR. 2002. Determination of vitamins A (retinol) and E (alpha -tocopherol) in foods by liquid chromatography: collaborative study. J AOAC Int 85 (2): 424-34
  10. 국립수의과학검역원. 2005: 비타민류 시험법, 축산물의 가공기준 및 성분규격. 국립수의과학검역원, 서울 : 80-115
  11. Wheeler LA, De Meo M, Kirby BD, et al. 1980 High-performance liquid chromatographic assay for measurement of cefoxitin in serum. J Chromatogr 183(3) : 357-362 https://doi.org/10.1016/S0378-4347(00)81717-X
  12. Rouan MC, Abadie F, Leclerc A, et al. 1083. Systematic approach to the determination of cephalospor in biological fluids by reversed-phase liquid chromatography. J Chromatogr 275(1) : 133-144 https://doi.org/10.1016/S0378-4347(00)84352-2
  13. Rouan MC. 1988. Microbore liquid chromatographic determination of cadralazine and cephalexin in plasma with large-volume injection. J Chromatogr 426 (2) : 335-344 https://doi.org/10.1016/S0378-4347(00)81960-X
  14. Emm TA, Leslie J, Chai M, et al. 1988. High-performance liquid chromatographic assay of cephalexin in serum and urine. J Chromatogr 427 (1) : 162-165 https://doi.org/10.1016/0378-4347(88)80116-6