Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Kinetic Modeling of Submerged Culture of A. blazei with Mixed Carbon Sources of Glucose and Dextrin

  • Na Jeong-Geol (Fuel Process Research Center, Korea Institute of Energy Research) ;
  • Kim Hyun-Han (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Chang Yong-Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Lee Sang-Jong (STR Biotech Co., Ltd.)
  • Published : 2006.09.01
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Abstract

A mathematical model has been proposed for the batch culture of Agaricus blazei with mixed carbon sources of glucose and dextrin. In the proposed model, the metabolism of A. blazei was divided into three parts: cell growth, exopolysaccharides (EPS) production, and another EPS production pathway activated by dextrin hydrolysis. Each pathway was described mathematically and incorporated into the mechanistic model structure. Batch cultures were carried out with six different carbon source compositions. Although parameters were estimated by using the experimental data from the two extreme cases such as glucose only and dextrin only, the model represented well the profiles of glucose, cell mass, and EPS concentrations for all the six different carbon source mixtures, showing a good interpolation capability. Of note, the lag in EPS production could be quite precisely predicted by assuming that the glucose-to-cell mass ratio was the governing factor for EPS production.

Keywords

References

  1. Bae, J. T., J. Sinha, J. P. Park, C. H. Song, and J. W. Yun. 2000. Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. J. Microbiol. Biotechnol. 10: 482-487
  2. Choi, K. H. 1999. Development of a new synthetic medium composition for the submerged culture of Phellinus linteus. Kor. J. Biotechnol. Bioeng. 14: 167-173
  3. Dong, Q., J. Yao, X.-T. Yang, and J.-N. Fang. 2002. Structural characterization of a water soluble ${\beta}$-D-glucan from fruiting bodies of A. blazei Murr. Carbohydr. Res. 337: 1417-1421 https://doi.org/10.1016/S0008-6215(02)00166-0
  4. Font, R. and J. M. Lopez. 1990. Kinetics of insoluble-substrate fermentation in mixed continuous-flow systems. Chem. Eng. Sci. 45: 3097-3109 https://doi.org/10.1016/0009-2509(90)80056-K
  5. Goldberg, D. E. 1989. Genetic Algorithms: In Search, Optimization and Machine Learning, Massachusetts, Addison-Wesley
  6. Goudar, C. T. and K. A. Strevett. 1998. Estimation of growth kinetics of Penicillum chrysogenum by nonlinear regression. Biochem. Eng. J. 1: 191-200 https://doi.org/10.1016/S1369-703X(98)00002-3
  7. Kawagishi, H., R. Katsumi, T. Sazawa, T. Mizuno, T. Hagiwara, and T. Nakamura. 1989. Cytotoxic steroids from the mushroom A. blazei. Pytochemistry 27: 2777-2779
  8. Kim, H. H., J.-G Na, G.-T. Chun, Y. K. Chang, S. J. Lee, and Y. H. Chung. 2004. Optimization of submerged culture conditions for mycelial growth and exopolysaccharides production by Agaricus blazei. J. Microbiol. Biotechnol. 14: 944-951
  9. Mizuno, M., M. Morimoto, K. Minate, and H. Tsuchida. 1998. Polysaccharides from A. blazei stimulate lymphocyte T-cell subsets in mice. Biosci. Biotech. Biochem. 62: 434-437 https://doi.org/10.1271/bbb.62.434
  10. Na, J.-G, H. H. Kim, G-T. Chun, Y. K. Chang, S. J. Lee, and Y H. Chung. 2005. Enhancement of $\beta$-D-glucans production by Agaricus blazei Murill nitrogen supplementation. J. Microbiol. Biotechnol. 15: 1388-1391
  11. Na, J.-G, Y. K. Chang, B. H. Chung, and H. C. Lim. 2002. Adaptive optimization of fed-batch culture of yeast by using genetic algorithms. Bioproc. Biosyst. Eng. 24: 299-308 https://doi.org/10.1007/s004490100251
  12. Park, J. P, S. W. Kim, H. J. Hwang, and J. W. Yun. 2001. Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production of Cordyceps militaris. Lett. Appl. Microbiol. 33: 76-81 https://doi.org/10.1046/j.1472-765X.2001.00950.x