Cybernetic Modeling of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Wood with Brettanomyces custersii

  • Shin Dong-Gyun (Department of Chemical and Biological Engineering, Korea University) ;
  • Yoo Ah-Rim (Department of Chemical and Biological Engineering, Korea University) ;
  • Kim Seung-Wook (Department of Chemical and Biological Engineering, Korea University) ;
  • Yang Dae-Ryook (Department of Chemical and Biological Engineering, Korea University)
  • Published : 2006.09.01

Abstract

The simultaneous saccharification and fermentation (SSF) process consists of concurrent enzymatic saccharification and fermentation. In the present cybernetic model, the saccharification process, which is based on the modified Michaelis-Menten kinetics and enzyme inhibition kinetics, was combined with the fermentation process, which is based on the Monod equation. The cybernetic modeling approach postulates that cells adapt to utilize the limited resources available to them in an optimal way. The cybernetic modeling was suitable for describing sequential growth on multiple substrates by Brettanomyces custersii, which is a glucose- and cellobiose-fermenting yeast. The proposed model was able to elucidate the SSF process in a systematic manner, and the performance was verified by previously published data.

Keywords

References

  1. Jones, K. and D. Kompala. 1999. Cybernetic model of the growth dynamic of Saccharomyces cerevisiae in batch continuous culture. J. Biotechnol. 71: 105-131 https://doi.org/10.1016/S0168-1656(99)00017-6
  2. Kompala, D., D. Ramkrishna, N. Jansen, and G. Tsao. 1986. Investigation of bacterial growth on mixed substrate: Experimental evaluation of cybernetic models. Biotechnol. Bioeng. 28: 1044-1055 https://doi.org/10.1002/bit.260280715
  3. Lin, J. Q., S. M. Lee, and Y. M. Koo. 2004. Model development for lactic acid fermentation and parameter optimization using genetic algorithm. J. Microbiol. Biotechnol. 14: 1163-1169
  4. Anonymous. 2005. Modeling and simulation of simultaneous saccharification and fermentation of paper mill sludge to lactic acid. J. Microbiol. Biotechnol. 15: 40-47
  5. Moon, H. J., J. S. Kim, K. K. Oh, S. W. Kim, and S. I. Hong. 2001. Kinetic modeling of simultaneous saccharification and fermentation for ethanol production using steam-exploded wood with glucose- and cellobiose-fermenting yeast, Brettanomyces custersii. J. Microbiol. Biotechnol. 11: 598-606
  6. Namjoshi, A. and D. Ramkrishna. 2001. Multiplicity and stability of steady states in continuous bioreactors: Dissection of cybernetic models. Chem. Eng. Sci. 56: 5593-5607 https://doi.org/10.1016/S0009-2509(01)00166-X
  7. Narang, A., A. Konopka, and D. Ramkrishna. 1997. Dynamic analysis of the cybernetic model for diauxic growth. Chem. Eng. Sci. 52: 2567-2578 https://doi.org/10.1016/S0009-2509(97)00073-0
  8. Park, S. W., Y. K. Hong, S. W. Kim, and S. I. Hong. 1999. Development of strain fermenting the glucose/cellobiose mixed sugar for simultaneous saccharification and fermentation of cellulosic material. Korean J. Appl. Microbiol. Biotechnol. 27: 145-152
  9. Philippidis, G, T. Smith, and C. Wyman. 1993. Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process. Biotechnol. Bioeng. 41: 846-853 https://doi.org/10.1002/bit.260410903
  10. Philippidis, G, D. Spindler, and C. Wyman. 1992. Mathematical modeling of cellulose conversion to ethanol by the simultaneous saccharification and fermentation process. Appl. Biochem. Biotechnol. 34/35: 543-556 https://doi.org/10.1007/BF02920577
  11. Ramkrishna, D. 1982 A cybernetic perspective of microbial growth, pp. 161-178. Foundations of Biochemical Engineering: Kinetics and Thermodynamics in Biological Systems. American Chemical Society, Washington, DC, U.S.A
  12. Schell, D. and P. Walter. 1991 Simultaneous saccharification and fermentation of corn stover hydrolysate to ethanol, pp. 72, Biotech. Symp. for Fuels and Chemicals, Colorado Springs
  13. South, C. R., D. A. L. Hogsett, and L. R. Lynd. 1995. Modeling simultaneous saccharification and fermentation of lignocellulose to ethanol in batch and continuous reactors. Enzyme Microb. Technol. 17: 797-803 https://doi.org/10.1016/0141-0229(94)00016-K
  14. Spindler, D., G. Philippidis, and C. Wyman. 1991. Key parameters in simultaneous saccharification and fermentation of biomass to ethanol, pp. 74, Biotech. Symp. for Fuels and Chemicals, Colorado Springs