The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Bioinformatic Analysis of NLS (Nuclear Localization Signals)-containing Proteins from Mollusks

생물정보학을 이용한 연체동물의 NLS (Nuclear Localization Signals) 포함 단백질의 분석

  • Lee, Yong-Seok (Department of Parasitology, Inje University College of Medicine and Department. of Malariology, PICR) ;
  • Kang, Se-Won (Department of Parasitology, Inje University College of Medicine and Department. of Malariology, PICR) ;
  • Jo, Yong-Hun (Department of Agricultural Biology, Chonnam National University) ;
  • Gwak, Heui-Chul (Department of Orthopedic Surgery, Pusan Paik-Hospital, Inje University College of Medicine) ;
  • Chae, Sung-Hwa (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Sang-Haeng (Department of Orthopedic Surgery, Pusan Paik-Hospital, Inje University College of Medicine) ;
  • Ahn, In-Young (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Hong-Seog (Department of Orthopedic Surgery, Pusan Paik-Hospital, Inje University College of Medicine) ;
  • Han, Yeon-Soo (Department of Agricultural Biology, Chonnam National University) ;
  • Kho, Weon-Gyu (Department of Parasitology, Inje University College of Medicine and Department. of Malariology, PICR)
  • 이용석 (인제의대 기생충학교실 및 백인제임상의학연구소 말라리아연구부) ;
  • 강세원 (인제의대 기생충학교실 및 백인제임상의학연구소 말라리아연구부) ;
  • 조용훈 (전남대학교 농업생명과학대학 농생물학과) ;
  • 곽희철 (인제의대 부산백병원 정형외과) ;
  • 채성화 (한국생명공학연구원 유전체연구센터) ;
  • 최상행 (한국생명공학연구원 유전체연구센터) ;
  • 안인영 (한국해양연구원 부설 극지연구소) ;
  • 박홍석 (한국생명공학연구원 유전체연구센터) ;
  • 한연수 (전남대학교 농업생명과학대학 농생물학과) ;
  • 고원규 (인제의대 기생충학교실 및 백인제임상의학연구소 말라리아연구부)
  • Published : 2006.12.30
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Abstract

Subcellular localization of a protein containing nuclear localization signals (NLS) has been well studied in many organisms ranging from invertebrates to vertebrates. However, no systematic analysis of NLS-containing proteins available from Mollusks has been reported. Here, we describe in silico screening of NLS-containing proteins using the mollusks database that contains 22,138 amino acids. To screen putative proteins with NLS-motif, we used both predict NLS and perl script. As a result, we have found 266 proteins containing NLS sequences which are about 1.2% out of the entire proteins. On the basis of KOG (The eukaryotic orthologous groups) analysis, we can't predict the precise functions of the NLS-containing proteins. However, we found out that these proteins belong to several types of proteins such as chromatin structure and dynamics, translation, ribosomal structure, biogenesis, and signal transduction mechanism. In addition, we have analysed these sequences based on the classes of mollusks. We could not find many from the species that are the main subjects of phylogenetic studies. In contrast, we noticed that cephalopods has the highest number of NLS-containing proteins. Thus, we have constructed mollusks NLS database and added these information and data to the mollusks database by constructing web interface. Taken together, these information will be very useful for those who are or will be studying NLS-containing proteins from mollusks.

연체동물 유래 아미노산 서열 22,138 개에서 NLS가 예측되는 아미노산 서열은 266 개였으며 이는 연체동물 전체 아미노산 중 1.2% 정도였다. 또한 현재 등재되어 있는 연체동물 8,314 종 중 NLS를 포함한 아미노산이 밝혀진 생물은 60여종에 불과 하였다. 현재 알려진 연체동물 서열 중에는 두족 강의 경우가 NLS를 포함한 아미노산이 많을 것으로 예측되었다.

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