Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Extracts of Centaurea bornmuelleri and Centaurea huber-morathii inhibit the growth of colon cancer cells in vitro

  • Sarker, Satyajit Dey (School of Biomedical Sciences, University of Ulster at Coleraine) ;
  • Shoeb, Mohammad (School of Pharmacy, The Robert Gordon University) ;
  • Celik, Sezgin (Department of Biology, Faculty of Science and Literature, Canakkale Onsekiz Mart University) ;
  • Jaspars, Marcel (Marine Natural Products Laboratory, Department of Chemistry, University of Aberdeen) ;
  • Nahar, Lutfun (School of Life Sciences, The Robert Gordon University) ;
  • Kong-Thoo-Lin, Paul (School of Life Sciences, The Robert Gordon University) ;
  • MacManus, Stephen M (School of Pharmacy, The Robert Gordon University)
  • Published : 2007.12.31
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Abstract

Plants from the genus Centaurea (C.) (Family: Asteraceae alt. Compositae), widely distributed in Asia, Europe and North America, have traditionally been used in the treatment of various ailments. As a part of our on-going studies on the plants from the genus C. for their phytochemistry and biological activities, extracts of the seeds of Turkish endemic C. species, C. bornmuelleri and C. huber-morathii, were tested for their cytotoxicity towards the CaCo2 colon cancer cell line as well as for the toxicity towards the brine shrimps, using the MTT and the brine shrimp lethality assays, respectively. Among the extracts, the MeOH extract of these plants showed significant toxicity towards the brine shrimps ($LD_{50}=55.2{\times}10^{-2}\;and\;42.4{\times}10^{-2}mg/ml$, respectively). The MeOH extract of both C. species also inhibited the growth of CaCo2 colon cancer cells in the MTT assay ($IC_{50}$=29.9 and 33.0 g/ml, respectively). As the most prominent activities in both assays were observed with the MeOH extracts, it can be assumed that the compound(s) responsible for these activities are polar in nature.

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