Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Eucommia ulmoides Extracts on Allergic Contact Dermatitis and Oxidative Damage Induced by Repeat Elicitation of DNCB

두충 추출물이 DNCB로 유발된 알레르기성 접촉피부염과 산화적 손상에 미치는 영향

  • Shon, Mi-Yae (Dept. of Food Science and Nutrition, Gyeongsang National University) ;
  • Nam, Sang-Hae (Dept. of Food Science, Jinju National University)
  • 손미예 (경상대학교 식품영양학과) ;
  • 남상해 (진주산업대학교 식품과학과)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Inhibitory effects of allergic contact dermatitis of hot water extract of Duchung (Eucommia ulmoides Oliver) leaf, bark and stem growing at Sancheong-gun were investigated for female BALB/c mouse induced by repeat elicitation of DNCB (2,4-dinitro-chlorobenzene). Skin reactions, consisting of increased ear thickness and the presence of ear inflammation, were observed in mice treated with DNCB and Duchung. Weight of lymph node, spleen and thymus in mice treated with Duchung extracts were lower than that of mouse treated with DNCB. Ear weight of mouse treated with Duchung extracts was decreased by increasing the concentration of sample as compared to control group and dropped as low as control level at 1,000 mg/kg. Ear thickness became thinner as test time on Duchung extract progressed. MDA (malondialdehyde) contents in liver tissue were not different in sample group with DNCB group, but were different in ear tissue. NO (nitric oxide) contents was decreased in Duchung extract groups at serum and ear tissues as compared to 1% DNCB group. In the present study, the results suggested that Duchung extract inhibits inflammatory response and oxidative damage induced DNCB allergen.

두충(잎, 껍질, 줄기)의 추출물이 DNCB로 감작된 4주령 BALB/c mouse 암컷에 유도된 접촉성 피부염의 억제효과를 조사하였다. 림프절, 비장 및 흉선의 무게는 두충 투여군이 DNCB 대조군보다는 낮게 나타났다. 두충 부위별 추출물을 투여한 군에서 귀의 무게는 두충 추출물의 농도가 높을수록 감소하는 경향을 보였으며, 대조군과 비교하여 무게에 함량 변화가 있었다. 두충의 부위별 추출물을 1,000 mg/kg 농도로 투여하면 귀의 무게는 대조군과 유사한 수준까지 낮아졌으며, 귀의 두께는 두충 추출물을 투여한 군에서 시간이 경과할수록 두께가 감소하는 경향을 나타내었다. MDA 함량은 DNCB 대조군과 두충 투여군을 비교하였을 경우에, 간 조직에서 차이가 나타나지 않았으나, 염증이 발생한 귀 조직에서는 차이가 나타났으며(p<0.05), NO 함량은 SW, ST 및 BT 그룹에서 염증이 억제되어 대조군과 마찬가지로 측정되지 않았다.

Keywords

References

  1. Kim MH, Kim MC, Park JS, Kim JW, Lee JO. 2001. The antioxidative effects of the water-soluble extracts of plants used as tea materials. Korean J Food Sci Technol 33: 12-18
  2. Kang SY, Hue SH, Kim SI. 1978. Immunologic aspects of hypersensitivity disease in Korea. Seoul J Medicine 19: 45-53
  3. Chio KU, Paek DM. 1995. Asthma and air pollution in Korea. Korean J Epidemiology 17: 64-75
  4. Tasaka K. 1986. Antiallergic drugs. Drugs of Today 22: 101-133
  5. Whang WK, Choi SB, Kim IH. 1996. Physiological activities of mixed extracts of Acantopanics senticosi Radicis Cortex and Eucommiae Cortex. Kor J Pharmacogn 27: 65-74
  6. Choe YH, Seo JH, Kim JS, Heo JH, Kim SG, Choe SU, Kim YS, Kim YG, Yu SY. 2003. Inhibitory effects of the stem bark extract of Eucommia ulmoides on the proliferation of human tumor cell lines. Kor J Pharmacogn 34: 308-313
  7. Jang HJ, Ra DY, Kim OC, Park JY. 1990. Volatile components of Du-Chung barks. J Korean Agric Chem Soc 33: 116-119
  8. Hwang JB, Yang MO, Shin HK. 1997. Survey for approximate composition and mineral content of medicinal herbs. Korean J Food Sci Technol 29: 671-679
  9. Kang JH, Park CJ, Yoon SY, Jeon SH, Her CY. 2005. Growth and morphological characteristics of soybean sprouts treated with leaf extracts of Thea sinensis L. and Eucommia ulmoides Oliver. Korean J Medicinal Corp Sci 13: 11-16
  10. Son KH, Lee JM, Chang SY, Lee KS. 2001. Isolation and quantitative determination of geniposide from the cortex of Eucommia ulmoides Oliver. Kor J Pharmacogn 32: 89-92
  11. Baek NI, Hahn JT, Ahn EM, Bang MH, Nam JY, Kwon BM. 1999. Isolation of Grb2-Shc interaction inhibitory compounds from the leaves of Eucommia ulmoides O. Kor J Pharmacogn 30: 202-206
  12. Baek NI, Ahn EM, Han JT, Lee DW, Sohn HO, Kwon BM. 1999. Isolation of monoamine oxidase B inhibitory compound from the leaves of Eucommia ulmoides Oliver. J Korean Soc Agric Chem Biotechnol 42: 166-169
  13. Choe SI, Lee YM, Heo TL. 2003. Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Korean J Biotechnol Bioeng 18: 282-288
  14. Kim NJ. 1995. Pharmacological activity of Eucommia ulmoides. Kor J Pharmacogen 26: 192-198
  15. Kim JH, Wang SG. 1997. Effects of mugwort, dried orange peel and Duchung on lipid metabolism in hyperlipidemia rats. Kor J Nutr 30: 895-903
  16. Yun JS, Jeong BH, Kim NY, Seong NS, Lee HY, Lee JH, Kim JD. 2003. Screening of 94 plant species showing ACE inhibitory activity. Korean J Medicinal Corp Sci 11: 246-251
  17. Choi GP, Chung BH, Lee DI, Lee HY, Lee JH, Kim JD. 2002. Screening of inhibitory activities on angiotensin converting enzyme from medicinal plants. Korean J Medicinal Corp Sci 10: 399-402
  18. Kim HY. 2000. Hypocholesterolemic and hypotensive activities of Eucommina ulmoides Oliver. Food Industry Nutr 5: 27-28
  19. Hwang WI. 1992. A study on the growth inhibition of human colon cancer cells by Eucommial leaf extract. Korean J Food Nutr 5: 13-21
  20. Hong ND, Rho YS, Won DH, Kim NJ, Cho BS. 1986. Studies on the anti-diabetic activity of Eucommia ulmoides Oliver. The Abstracts of 17th Annual Convention of The Korean Society of Pharmacognosy. p 87
  21. Oh HS, Kim HC, Lee SI, Ahn DK. 1995. Effects of Eucommiae Cortex and Folium on the ovariectomized rat as the model of postmenopaual osteoporosis. J Herbology 10: 59-68
  22. Lee IK, Kim JG. 2000. Effects of extract of Eucommia ulmoides Oliver on the reduction of lead and cadmium in organs of rats. J Korean Public Health Assoc 26: 22-28
  23. Hong ND, Rho YS, Kim JW, Won DH, Kim NJ, Cho BS. 1988. Studies on the general pharmacological activities of Eucommia ulmoides Oliver. Kor J Pharmacogn 19: 102- 110
  24. Holliday MR, Dearman RJ, Kimber I, Coleman JW. 1992. Sensitization of mice to chemical allergens modulates the responsiveness of isolated mast cell to IgE-dependent activation. Immunology 78: 508-510
  25. Dal-Pizzol F, Klamt F, Vianna M, Schroder N, Quevedo J, Benfato MS, Moreira JC, Walz R. 2000. Lipid peroxidation in hippocampus early and late after status epilecticus induced by pilocarpine or kainic acid in Wistar rats. Neurosci Letter 291: 179-182 https://doi.org/10.1016/S0304-3940(00)01409-9
  26. Klamt F, Dal-Pizzol F, Frota MLC, Walz R, Andrades ME, Silva EG, Brentani R, Izquierdo I, Moreira JCF. 2001. Imbalance of antioxidant defence in mice lacking cellular prion protein. Free Radic Biol Med 30: 1137-1144 https://doi.org/10.1016/S0891-5849(01)00512-3
  27. Kwon OS. 1996. The effect of Yunkyopaedocksan-gami on allergic contact dermatitis on mice induced by DNCB. PhD Dissertation. Dongguk University
  28. Uchiyama M, Mihara M. 1978. Determination of malondialdehyde precursor in tissue by TBA test. Anal Biochem 86: 271-278 https://doi.org/10.1016/0003-2697(78)90342-1
  29. Chi YS, Cheon BS, Kim HP. 2001. Effect of wogonin, a plant flavone from Scutellafia radix, on the suppression of cyclooxygenase-2 and the induction of inducile nitric oxide synthase in lipopolysaccharide-treated RAW264.7 cell. Biochem Pharmacol 61: 1195-1203 https://doi.org/10.1016/S0006-2952(01)00597-4
  30. Ishizaka K. 1984. Regulation of IgE synthesis. Annu Rev Immunol 2: 159-162 https://doi.org/10.1146/annurev.iy.02.040184.001111
  31. Ohmori Y, Ito M, Mizutani H, Katada T, Konishi H. 1995. Antiallergic constituents from Oolong tea stem. Biol Pharm Bull 18: 683-686 https://doi.org/10.1248/bpb.18.683
  32. Coombs RRA, Gell PGH, Lachmam PJ. 1975. Clinical aspects of immunology. Blackwell Scientific Publications, Oxford. p 761-766
  33. Arts JHE, Droge SCM, Bloksma N, Kuper CF. 1996. Local lymph node activation in rats after dermal application of the sensitizers 2,4-dinitrochlorobenzene and trimellitic anhydride. Food Chem Toxicol 34: 55-6 https://doi.org/10.1016/0278-6915(95)00089-5
  34. Gerberick GF, Cruse LW, Ryan CA. 1999. Local lymph node assay: differentiating allergic and irritant responses using flow cytometry. Eur J Med Res 19: 48-55
  35. Sikorski EE, Gerberick GF, Ryan CA, Miller CM, Ridder GM. 1996. Phenotypic analysis of lymphocyte subpopulations in lymph nodes draining the ear following exposure to contact allergens and irritants. Fundamental Appl Toxicol 34: 25-35 https://doi.org/10.1006/faat.1996.0172
  36. Suda A, Yamashita M, Tabei M, Taguchi K, Vohr HW, Tsutsui N, Suzuki R, Kikuchi K, Sakaguchi K, Mochizuki K, Nakamura K. 2002. Local lymph node assay with non-radioisotope alternative endpoints. J Toxicol Sci 27: 204-218 https://doi.org/10.2131/jts.27.205
  37. Park MH, Choi C, Bae MJ. 2000. Effect of polyphenol compounds from persimmon leaves (Diospyros kakifolium) on allergic contact dermatitis. J Korean Soc Food Sci Nutr 29: 111-115
  38. Fleming I, Busse R. 2004. The physiology of nitric oxide: control and consequences. Curr Med Chem Anti-Allergy Agents 3: 189-205 https://doi.org/10.2174/1568014043355366
  39. Dedon PC, Tannenbaum SR. 2004. Reactive nitrogen species in the chemical biology of inflammation. Arch Biochem Biophys 423: 12-22 https://doi.org/10.1016/j.abb.2003.12.017
  40. Hogg N, Kalyanaraman B, Darley-Usmar V. 1995. Oxidant and antioxidant effects of nitric oxide and superoxide in the vasculature. In The Oxygen Paradox. Cleup University Press, Paradona, Italy. p 158-163

Cited by

  1. Effects of Portulacae Herba (PH) on Allergic Contact Dermatitis (ACD) Induced by DNCB in Mice vol.27, pp.3, 2014, https://doi.org/10.6114/jkood.2014.27.3.115
  2. The Anti-Inflammatory Effects of Picea wilsonii Mast on HaCaT Cells vol.48, pp.4, 2016, https://doi.org/10.15324/kjcls.2016.48.4.365
  3. Effect of Extracts from Acanthopanax senticosus and Eucommia ulmoides on Shelf-Life and Quality of Wet Noodle vol.39, pp.6, 2010, https://doi.org/10.3746/jkfn.2010.39.6.887
  4. Anti-Allergic Activities of Fermented Eriobotrya japonica and Saurus chinensis Extracts in 2,4-Dinitrochlorobezene-Induced BALB/c Mice vol.39, pp.11, 2010, https://doi.org/10.3746/jkfn.2010.39.11.1611
  5. Effect of Pine needle Ethanol Extracts on the Inhibitory Activity of Atopic Dermatitis vol.28, pp.2, 2013, https://doi.org/10.7841/ksbbj.2013.28.2.123
  6. Effects of Hyeongbangpaedok-san (HBPDS) on Allergic Contact Dermatitis (ACD) induced by DNFB in Mice vol.26, pp.2, 2014, https://doi.org/10.7730/JSCM.2014.26.2.180
  7. Characterization and Transdermal Delivery of Ethosomes Loaded with Eucommia ulmoides Extract vol.24, pp.6, 2013, https://doi.org/10.14478/ace.2013.1090
  8. 현삼이 DNCB로 유발된 알레르기성 접촉성 피부염에 미치는 영향 vol.24, pp.3, 2011, https://doi.org/10.6114/jkood.2011.24.3.001
  9. DNCB 유도 BALB/c 생쥐에서 어성초 혼합 한방추출물의 항아토피 효과 vol.29, pp.2, 2007, https://doi.org/10.12925/jkocs.2012.29.2.1
  10. 참모자반 물 추출물의 항아토피 효과 vol.43, pp.2, 2007, https://doi.org/10.4014/mbl.1504.04006
  11. 杜冲의 토끼 음경해면체 평활근 이완효과 vol.29, pp.6, 2007, https://doi.org/10.15188/kjopp.2015.12.29.6.485
  12. 항알레르기에 대한 Pediococcus inopinatus Y2 유산균 발효 두유 커드의 치료 효과 vol.29, pp.4, 2007, https://doi.org/10.5352/jls.2019.29.4.478
  13. Development of a Natural Matrix Hybrid Hydrogel Patch and Evaluation of Its Efficacy against Atopic Dermatitis vol.10, pp.23, 2007, https://doi.org/10.3390/app10238759