Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Electrochemical Assay of Neurotransmitter Glycine in Brain Cells

  • Ly, Suw-Young (Biosensor Research Institute, Seoul National University of Technology) ;
  • Kim, Dong-Young (Advanced Scientific Experiment Group in DaeJin High School)
  • Published : 2007.04.20
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Abstract

Neurotransmitter glycine in the nano gram range was analyzed using a paste electrode (PE) in cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV). An anodic peak caused by oxidation of the glycine ion appeared at the 0.4 V (versus Ag/AgCl/KCl) potential in a 0.1 M NH4H2PO4 electrolyte solution. At optimized conditions, the working range of the SWSV and CV concentration was found to be 5-60 ngL-1 glycine; precision of R2 = 0.9816 (SWSV) and 0.9986 (CV); and detection limit of 0.65 ngL-1 (5.82 × 10-12 molL-1) (S/N = 3). The optimized conditions were applied to an assay in a fish brain tissue and a living brain cell in real time.

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References

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