Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Dietary Selfheal (Prunella vulgaris) Water Extracts and Its Culture Fluid with Lactobacillus rhamnosus on Growth and Immune Responses of Juvenile Olive Flounder

두메꿀풀(Prunella vulgaris) 열수추출액과 미생물(Lactobacillus rhamnosus) 배양액의 사료 내 첨가가 넙치 치어의 성장과 면역반응에 미치는 영향

  • Kim, Sung-Sam (Faculty of Applied Marine Science, Cheju National University) ;
  • Galaz, German Bueno (Faculty of Applied Marine Science, Cheju National University) ;
  • Heo, Moon-Soo (Faculty of Applied Marine Science, Cheju National University) ;
  • Kim, Gi-Young (Faculty of Applied Marine Science, Cheju National University) ;
  • Choi, Kwang-Sik (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Ki-Wan (Faculty of Applied Marine Science, Cheju National University) ;
  • Yeo, In-Kyu (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Kyeong-Jun (Faculty of Applied Marine Science, Cheju National University)
  • 김성삼 (제주대학교 해양과학대학 해양과학부) ;
  • ;
  • 허문수 (제주대학교 해양과학대학 해양과학부) ;
  • 김기영 (제주대학교 해양과학대학 해양과학부) ;
  • 최광식 (제주대학교 해양과학대학 해양과학부) ;
  • 이기완 (제주대학교 해양과학대학 해양과학부) ;
  • 여인규 (제주대학교 해양과학대학 해양과학부) ;
  • 이경준 (제주대학교 해양과학대학 해양과학부)
  • Published : 2007.10.31
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Abstract

This study investigated the effects of dietary supplementation with hot water extracts (HE) of selfheal (Prunella vulgaris) and Lactobacillus rhamnosus culture fluid (LF) on the growth and non-specific immune responses of juvenile olive flounder. A total of 270 fish ($5.07{\pm}0.01g$, average $weight{\pm}SD$) were divided randomly into nine groups, and three groups were fed one of three isonitrogenous (51% crude protein) and isocaloric (17.6 MJ/kg) diets. The diets contained no supplement, 50 mL hot water extract, or 50 mL L. rhamnosus fluid (designated as diets CON, HE and LF, respectively). During the 8-week feeding trial, the growth, feed utilization and survival of the fish were not significantly affected by the experimental diets. There were no significant differences in the hematocrit and hemoglobin of fish fed each of the experimental diets. However, the serum alanine aminotransferase and aspartate aminotransferase activity were lower with the dietary supplements containing HE and LF. The alanine aminotransferase activity of fish fed the HE diet was significantly lower than that of fish fed the control diet. The hematocrit, hemoglobin, and nitroblue tetrazolium (NBT) activity were higher 3 hr after feeding than 24 hr afterwards. The NBT activity of fish fed the HE and LF diets were significantly higher than that of fish fed the control diet 3 hr after feeding. The findings suggest that dietary supplementation with HE and LF could enhance the nonspecific immune responses of juvenile olive flounder.

Keywords

References

  1. Anderson, D.P. and A.K. Siwicki. 1995. Basic haematology and serology for fish health programs. In: Diseases in Asian Aquaculture II. Shariff, M., J.R. Arthur and R.P. Subasinghe, eds. Asian Fisheries Society, Manila, Philippines, 1-185
  2. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Arlington, Virginia, 1-1298
  3. Bhowmilk, P.C. and J.D. Doll. 1984. Allelopathic effect of annual weed residues on growth and nutrient uptake of corn and soybeans. Agron. J., 76, 838-388
  4. Cho, M.K., Y.J. Kim, D.Y. Shin and T.S. Choi. 2004. Cell scattering activity of natural plant extracts. Kor. J. Pharmacogn., 35, 62-79
  5. Chung, I.M. 1994. Allelopathy and autotoxicity studies and allelochemicals isolation and identification of alfalfa (Medicago sativa). Ph.D. Thesis, University of Illinois, USA
  6. Folch, J., M. Lee and G.H. Sloane-Stanley, 1959. A simple method for the isolation and purification of total lipids from animal tissues. J. BioI. Chem., 226, 497-509
  7. Fuller, R. and G.R. Gibson. 1997. Modification of the intestinal micro flora using probiotics and prebiotics. Scand. J. GastroenteroI., 222, 28-31
  8. Garling, D.L. Jr. and R.P. Wilson. 1976. Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus. J. Nutr., 106, 1368-1375 https://doi.org/10.1093/jn/106.9.1368
  9. Gopalakannan, A. and V. Arui. 2006. Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaulture, 255, 179-187 https://doi.org/10.1016/j.aquaculture.2006.01.012
  10. Heo, G.J., K.S. Shin and M.H. Lee. 1992. Diseases of aquaculture animals and prevention of drug residues. Kor. J. Food Hygiene, 7, S7-S19
  11. Iwama, G. and T. Nakanishi. 1996. Organ, Pathogen, and Environment. The Fish Immune System. Academic Press, San Diego, 1-380
  12. Jung, D.S. and N.H. Lee. 2007. Antimicrobial activity of the aerial part (leaf and stem) extracts of Cnidium officinale Makino, a Korean medicinal herb. Kor. J. Microbiol. Biotechnol., 35, 30-35
  13. Jeong, J.H., D.E. Jeong, S.J. Lee, K.J. Seul, C.M. Ryu, S.H. Park and S.Y. Ghim. 2007. The effects of wood vinegar on growth and resistance of peppers. Kor. J. Microbiol. Biotechnol., 35, 41-44
  14. Kajita, Y, M. Sakai, S. Atsuta and M. Kobayashi. 1990. The immunomodulatory effects of levamisole on rainbow trout, Oncorhynchus mykiss. Fish Pathol., 25, 93-98 https://doi.org/10.3147/jsfp.25.93
  15. Kim, K.J., I.M. Chung, K.H. Kim and J.K. Anh. 1993. Allelopathic influence of alfalfa and residues on soybean and com. Kor. J. Crop Sci., 39, 295-305
  16. Kim, K.W. and S.Y. Kim. 2005. Herbicidal and fungicidal activities of methanol extracts from native plants in Korea. Kor. J. Weed Sci., 25, 221-232
  17. Kim, S.M. 2006. Herbicidal activity of Korean native plants (IV). Kor. J. Pesticide Sci., 10, 225-229
  18. Kim, S.S., G.B. Galaz, K.J. Lee and YD. Lee. 2006. Effects of dietary supplementation of Spirulina and astaxanthin for juvenile olive flounder, Paralichthys olivaceus in low temperature season. J. Aquacult., 19, 57-63
  19. Kim, TJ. 1996. Korean Resoures Plants I. Seoul National University Press, Korea, 11-21
  20. Kim, Y.C., K.W. Kim, S.H. Lee, GJ. Park, O.E. Okorie, Y.J. Kang and S.C. Bai. 2006. Effects of dietary $\beta$-1,3 glucan on growth and immune responses in juvenile olive flounder, Paralichthys olivaceus. J. Aquacult., 19, 247-253
  21. Kimura, K., A.L. McCartney, M.A. McConell and G.W. Tannock. 1997. Analysis of fecal populations of bifidobacteria and lactobacilli and investigation of the immunological response of their human hosts to the predominant strains. Appl. Environ. Microbiol., 63, 3394-3398
  22. Kitao, T. and T. Yoshida. 1986. Effect of an immunopotentiator on Aeromonas salmonicida infection in rainbow trout (Salmo gairdneri). Vet. Immunol. Immunopathol., 12, 287-291 https://doi.org/10.1016/0165-2427(86)90132-7
  23. Kumari, J. and P.K. Sahoo. 2005. Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish, Clarias batracus. Fish Shellfish Immunol., 19, 307-316 https://doi.org/10.1016/j.fsi.2005.01.008
  24. Kwon, O.E., S.W. Lee, M.Y. Chung, Y.H. Kim, M.C. Rho, H.S. Lee and Y.K. Kim. 2002. Inhibitory effects of natural plant extracts on ICAM-1/LFA-1 mediated adhesion of HL-60 cells. Kor. J. Pharmacogn., 33, 343-351
  25. Lee, Y.N. 2002. Flora of Korea. 4th ed. Kyo-Hak Publishing Co. Ltd., Seoul, Korea
  26. McPhearson, R.M., A. DePaola, S.R. Zywno, J.M.L. Motes and A.M. Guarino. 1991. Antibiotic resistance in gram-negative bacteria from cultured catfish and aquaculture ponds. Aquaculture, 99, 203-211 https://doi.org/10.1016/0044-8486(91)90241-X
  27. Min, S.K., YK. Park, J.H. Park, S.H. Jin and K.W. Kim. 2004. Screening of antibacterial activity from hot water extracts of indigenous plants. J. Life Sci., 14, 951-962 https://doi.org/10.5352/JLS.2004.14.6.951
  28. Moon, T.C., H.J. Jung, E.K. Lee, H.Y Park, S.J. Jeon, K.H. Son, H.P. Kim, K.H. Bae, S.S. Kang, D.Y. Kwon and H.W. Chang. 2003. Screening of arachidonic acid cascade related enzymes inhibitors from Korean indigenous plants (1). Kor. J. Pharmacogn., 34, 109-117
  29. Moon, Y.G., K.S. Choi, K.J. Lee, G.Y Kim and M.S. Heo. 2006. Screening of anti oxidative and antibacterial activity from hot water extracts of indigeous plants, Jeju-Island. Kor. J. BiotechnoI. Bioeng., 21, 164-169
  30. Pan, C.H., Y.H. Chien and B. Hunter, 2003. The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin. J. Exp. Mar. BioI. Ecol., 297, 107-118 https://doi.org/10.1016/j.jembe.2003.07.002
  31. Panigrahi, A., V. Kiron, S. Satoh, I. Hirono, T. Kobayashi, H. Sugita, J. Puangkaew and T. Aoki, 2007. Immune modulation and expression of cytokine genes in rainbow trout Oncorhynchus mykiss upon probiotic feeding. Dev. Comp. ImmunoI., 31, 372-382 https://doi.org/10.1016/j.dci.2006.07.004
  32. Park, S.Y., Y.T. Ko, HK Jeong, J.O. Yang, H.S. Chung, Y.B. Kim and G.E. Ji. 1996. Effect of various lactic acid bacteria on the serum cholesterol levels in rats and resistance to acid, bile and antibiotics. Kor. J. Appl. Microbiol. Biotechnol., 24, 304-310
  33. Pham, M.A., K.J. Lee, BJ. Lee, S.J. Lim, S.S. Kim, Y.D. Lee, M.S. Heo and K.W. Lee. 2006. Effects of dietary Hizikia fusiformis on growth and immune responses in juvenile olive flounder (Paralichthys olivaceus). Asian.-Aust. J. Anim. Sci., 19, 1769-1775 https://doi.org/10.5713/ajas.2006.1769
  34. Pickering, A.D. 1992. Rainbow trout husbandry: management of the stress response. Aquaculture, 100, 125-139 https://doi.org/10.1016/0044-8486(92)90354-N
  35. Rice, E.L. 1984. Allelopathy, 2nd ed. Academic Press, Orlando, USA, 1-267
  36. Rumsey, G.L., A.K. Siwicki, D.P. Anderson and P.R. Bowser. 1994. Effect of soybean protein on serological response, non-specific defense mechanisms, growth, and protein utilization in rainbow trout. Vet. Immunol. Immunopathol., 41, 323-339 https://doi.org/10.1016/0165-2427(94)90105-8
  37. Sakai, M., H. Kamiya, S. Ishii, S. Atsuta and M. Kobayashi. 1992. The immunostimulating effects of chitin in rainbow trout, Oncorhynchus mykiss. In: Diseases in Asian Aquaculture. Shariff, M., R.P. Subasighe and J.R. Arthur, eds. Asian Fisheries Society, Manila, Philippines, 413-41
  38. Sakai, M., T. Otubo, S. Atsuta and M. Kobayashi. 1993. Enhancement of resistance to bacterial infection in rainbow trout, Oncorhynchus mykiss (Walbaum), by oral administration of bovine lactoferrin. J. Fish Dis., 16, 239-247 https://doi.org/10.1111/j.1365-2761.1993.tb01253.x
  39. Sakai, M., S. Atsuta and M. Kobayashi. 1995. The activation of leucocytes in rainbow trout, Oncorhynchus mykiss, by oral administration of Clostridium butyricum bacterin. In: Diseases in Asian Aquaculture. Shariff, M., R.P. Subasighe and J.R. Arthur, eds. Asian Fisheries Society, Manila, Philippines, 433-437
  40. Secombes, C.J, L.J. Hardie and G. Daniels. 1996. Cytokine in fish: an update. Fish Shellfish Immunol., 6, 291-304 https://doi.org/10.1006/fsim.1996.0030
  41. Skerget, M., P. Kotnik, M. Hadolin, A.R. Hras, M. Simonic and Z. Knez. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and the antioxidant activities. Food Chem., 89, 191-198 https://doi.org/10.1016/j.foodchem.2004.02.025
  42. Son, K.H., H.J. Son, J.H. Jeong and S.T. Kwon. 2005. Screening of inhibitory activity of seed germination from wild plant extracts. J. Kor. Flower Res. Soc., 13, 207-220
  43. Tannock, G.W., C. Crichton, G.W. Welling, J.P. Koopman and T. Midtvedt. 1988. Reconstitution of the gastrointestinal micro flora of lactobacillus-free mice. Appl. Environ. Microbial., 54, 2971-2975
  44. Watanuki, H., K. Ota, A.C.M. A.R. Tassakka, T. Kato and M. Sakai. 2006. Immunostimulant effects of dietary Spirulina platensis on carp, Cyprinus carpio. Aquaculture, 258, 157-163 https://doi.org/10.1016/j.aquaculture.2006.05.003
  45. Yang, M.S., Y.L. Ha, S.H. Nam, S.U. Choi and D.S. Jang. 1995. Screening of domestic plants with antibacterial activity. J. Kor. Soc. Appl. BioI. Chem., 38, 584-589
  46. Yano, T., R.E.P. Mangindaan and H. Matsuyama. 1989. Enhancement of the resistance of carp Cyprinus carpio to experimental Edwardsiella tarda infection, by some ${\beta}$-1,3-glucans. Nippon Suisan Gakkaishi, 55, 1815-1819 https://doi.org/10.2331/suisan.55.1815
  47. You, S.J., J.K. Cho, S.G. Hwang and K.C. Heo. 2005. Probiotic characteristics of Lactobacillus rhamnosus isolated from kefir. Kor. J. Food Sci. Ani. Resour., 25, 357-364
  48. Yu, H.N., K.H. Cho, D.E. Sok and T.S. Jeong. 2003. Inhibitory effects of natural plant extracts on lipoprotein-associated phospholipase A2, plateletactivating factor acetylhydrolase. Kor. J. Pharmacogn., 34, 100-108

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